Shared Resources Core

共享资源核心

• 批准号: 10732989
• 负责人: FENG CHEN
• 金额: $ 14万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10732989
• 关键词: ATAC-seq; Address; Anatomy; Atlases; Bioinformatics; Biological Assay; Biological Markers; Cells; Characteristics; Clinical; Complex; DNA; Data; Development; Drug resistance; Effectiveness; Ensure; Future; Genitourinary system; Genomics; Goals; Heterogeneity; Human; Image; Immunofluorescence Immunologic; Individual; Infrastructure; Maps; Mission; Modeling; Molecular; Molecular Profiling; Monitor; Oncogenes; Predictive Value; Procedures; Productivity; Proteins; Proteomics; RNA; Research; Research Design; Research Project Grants; Resistance; Resolution; Resource Sharing; Resources; Sampling; Services; Speed; Stains; Standardization; Stromal Cells; Structure; Supervision; Surveys; Technology; Testing; Tissues; Treatment outcome; United States National Institutes of Health; Universities; Validation; Visualization; Washington; Work; biomarker identification; cancer genome; clinical imaging; cost; exome sequencing; genome sequencing; improved; in vivo; individual response; molecular imaging; multiple omics; neoplastic cell; new technology; patient derived xenograft model; patient engagement; phosphoproteomics; potential biomarker; pre-clinical; programs; radiological imaging; senescence; single nucleus RNA-sequencing; stress reduction; targeted imaging; therapy resistant; tool; trait; transcriptome; transcriptome sequencing; transcriptomics; treatment response; tumor; tumor growth

SHARED RESOURCES CORE: SUMMARY The Shared Resources Core (SRC) is structured as a physical shared resource that will support both Research Projects, the PDX Core, as well as pilot and trans-Network projects. The most critical goal of the SRC is to provide services and resources that are either unavailable to individual research teams within PDXNet or that are unable to meet the cost, technical, and speed requirements of the Projects and other components at WU- PDTC. Other important missions for the SRC include optimization of the procedures for project/sample-specific requirements, standardization of the assays to ensure data consistency, and incorporation of the latest technologies. This centralized arrangement takes full advantage of the expertise and infrastructure existing at our institute developed through participation in several large-scale NIH consortia, including HTAN (Human Tumor Atlas Network), SenNet (Senescence Network), GUDMAP (GenitoUrinary Development Molecular Anatomy Project), CPTAC (Clinical Proteomic Tumor Analysis Consortium), PE-CGS (Participant Engagement and Cancer Genome Sequencing), CRIP (Co-Clinical Imaging Research Program), and PDXNet, in order to increase effectiveness and efficiency, avoid duplicated effort, and reduce stress on individual projects. SRC will be tightly integrated with the Research Projects, Pilot and Trans-Network Projects, the PDX Core, and the Bioinformatics Core under the supervision of the Administrative Core. Detailed characterization of the PDX tumors at the DNA, RNA, and protein levels will be important for validation, model selection, revelation of molecular changes in response to treatment, illustration of mechanisms of treatment resistance, as well as the identification of biomarkers and targets for future treatment testing. To accomplish these goals, the SRC will work with the Research Projects and other Cores to perform two main types of assays: Omics and imaging. For omics, the SRC will cover bulk and single cell omics, including whole exome sequencing (WES), bulk RNA-seq, single nucleus RNA-seq and ATAC-seq (snRNA-seq and snATAC-seq), spatial transcriptomics (Visium), as well as global proteomics and phospho-proteomics. For imaging, the SRC covers multiplex target imaging (CODEX/Phenocycler), in vivo preclinical molecular and radiologic imaging, and standard immunofluorescence (IF) staining. The SRC is also tasked with adjusting to the changing needs of the projects as well as introducing new technologies and tools to assist the Projects and to accomplish the mission of the center.

共享资源核心：摘要 共享资源核心（SRC）被构造为物理共享资源，将支持研究 项目、PDX 核心以及试点和跨网络项目。 SRC 最重要的目标是 提供 PDXNet 内的各个研究团队无法获得的服务和资源，或者 无法满足 WU- 项目和其他组件的成本、技术和速度要求 PDTC。 SRC 的其他重要任务包括优化项目/样本特定的程序 要求、测定标准化以确保数据一致性，并纳入最新的 技术。这种集中安排充分利用了现有的专业知识和基础设施 我们的研究所是通过参与多个大型 NIH 联盟而发展起来的，其中包括 HTAN（人类肿瘤 Atlas Network）、SenNet（衰老网络）、GUDMAP（泌尿生殖发育分子解剖学） 项目）、CPTAC（临床蛋白质组学肿瘤分析联盟）、PE-CGS（参与者参与和 癌症基因组测序）、CRIP（联合临床影像研究计划）和 PDXNet，以增加 有效性和效率，避免重复工作，并减少单个项目的压力。 SRC将被紧紧地 与研究项目、试点和跨网络项目、PDX 核心和生物信息学集成 核心受行政核心监督。 PDX 肿瘤 DNA 的详细表征， RNA 和蛋白质水平对于验证、模型选择、揭示分子变化非常重要 对治疗的反应，治疗抵抗机制的说明，以及识别 未来治疗测试的生物标志物和目标。为了实现这些目标，SRC 将与 研究项目和其他核心执行两种主要类型的分析：组学和成像。对于组学来说， SRC 将涵盖批量和单细胞组学，包括全外显子组测序 (WES)、批量 RNA 测序、单细胞组学 细胞核 RNA-seq 和 ATAC-seq（snRNA-seq 和 snATAC-seq）、空间转录组学 (Visium) 以及 全球蛋白质组学和磷酸蛋白质组学。对于成像，SRC 涵盖多重目标成像 (CODEX/Phenocycler)、体内临床前分子和放射成像以及标准免疫荧光 (IF) 染色。 SRC 还负责根据项目不断变化的需求进行调整，并引入 协助项目并完成中心使命的新技术和工具。