Shared Resources Core

共享资源核心

• 批准号: 10732989
• 负责人: FENG CHEN
• 金额: $ 14万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10732989
• 关键词: ATAC-seq; Address; Anatomy; Atlases; Bioinformatics; Biological Assay; Biological Markers; Cells; Characteristics; Clinical; Complex; DNA; Data; Development; Drug resistance; Effectiveness; Ensure; Future; Genitourinary system; Genomics; Goals; Heterogeneity; Human; Image; Immunofluorescence Immunologic; Individual; Infrastructure; Maps; Mission; Modeling; Molecular; Molecular Profiling; Monitor; Oncogenes; Predictive Value; Procedures; Productivity; Proteins; Proteomics; RNA; Research; Research Design; Research Project Grants; Resistance; Resolution; Resource Sharing; Resources; Sampling; Services; Speed; Stains; Standardization; Stromal Cells; Structure; Supervision; Surveys; Technology; Testing; Tissues; Treatment outcome; United States National Institutes of Health; Universities; Validation; Visualization; Washington; Work; biomarker identification; cancer genome; clinical imaging; cost; exome sequencing; genome sequencing; improved; in vivo; individual response; molecular imaging; multiple omics; neoplastic cell; new technology; patient derived xenograft model; patient engagement; phosphoproteomics; potential biomarker; pre-clinical; programs; radiological imaging; senescence; single nucleus RNA-sequencing; stress reduction; targeted imaging; therapy resistant; tool; trait; transcriptome; transcriptome sequencing; transcriptomics; treatment response; tumor; tumor growth

SHARED RESOURCES CORE: SUMMARY The Shared Resources Core (SRC) is structured as a physical shared resource that will support both Research Projects, the PDX Core, as well as pilot and trans-Network projects. The most critical goal of the SRC is to provide services and resources that are either unavailable to individual research teams within PDXNet or that are unable to meet the cost, technical, and speed requirements of the Projects and other components at WU- PDTC. Other important missions for the SRC include optimization of the procedures for project/sample-specific requirements, standardization of the assays to ensure data consistency, and incorporation of the latest technologies. This centralized arrangement takes full advantage of the expertise and infrastructure existing at our institute developed through participation in several large-scale NIH consortia, including HTAN (Human Tumor Atlas Network), SenNet (Senescence Network), GUDMAP (GenitoUrinary Development Molecular Anatomy Project), CPTAC (Clinical Proteomic Tumor Analysis Consortium), PE-CGS (Participant Engagement and Cancer Genome Sequencing), CRIP (Co-Clinical Imaging Research Program), and PDXNet, in order to increase effectiveness and efficiency, avoid duplicated effort, and reduce stress on individual projects. SRC will be tightly integrated with the Research Projects, Pilot and Trans-Network Projects, the PDX Core, and the Bioinformatics Core under the supervision of the Administrative Core. Detailed characterization of the PDX tumors at the DNA, RNA, and protein levels will be important for validation, model selection, revelation of molecular changes in response to treatment, illustration of mechanisms of treatment resistance, as well as the identification of biomarkers and targets for future treatment testing. To accomplish these goals, the SRC will work with the Research Projects and other Cores to perform two main types of assays: Omics and imaging. For omics, the SRC will cover bulk and single cell omics, including whole exome sequencing (WES), bulk RNA-seq, single nucleus RNA-seq and ATAC-seq (snRNA-seq and snATAC-seq), spatial transcriptomics (Visium), as well as global proteomics and phospho-proteomics. For imaging, the SRC covers multiplex target imaging (CODEX/Phenocycler), in vivo preclinical molecular and radiologic imaging, and standard immunofluorescence (IF) staining. The SRC is also tasked with adjusting to the changing needs of the projects as well as introducing new technologies and tools to assist the Projects and to accomplish the mission of the center.

共享资源核心：摘要 共享资源核心（SRC）的结构是一种物理共享资源，将支持这两种研究 项目，PDX核心以及试点和跨网络项目。 SRC最关键的目标是 提供PDXNET中各个研究团队无法获得的服务和资源，或者 无法满足WU-项目和其他组件的成本，技术和速度要求 PDTC。 SRC的其他重要任务包括优化项目/样本特定的程序 要求，分析的标准化以确保数据一致性以及最新的合并 技术。这种集中安排充分利用了现有的专业知识和基础设施 我们的研究所通过参加了包括HTAN（人类肿瘤 Atlas Network），Sennet（衰老网络），GUDMAP（泌尿生殖器发育分子解剖结构 项目），CPTAC（临床蛋白质组学分析联盟），PE-CGS（参与者参与和 癌症基因组测序），CRIP（共插入成像研究计划）和PDXNET，以增加 有效性和效率，避免重复的努力，并减少对单个项目的压力。 SRC会紧紧 与研究项目，飞行员和跨网络项目，PDX核心以及生物信息学集成在一起 在行政核心的监督下核心。 DNA处PDX肿瘤的详细表征， RNA和蛋白质水平对于验证，模型选择，分子变化的启示至关重要 对治疗的反应，治疗耐药性机制的例证以及鉴定 生物标志物和未来治疗测试的靶标。为了实现这些目标，SRC将与 研究项目和其他核心进行两种主要分析类型：OMICS和Imaging。对于Omics， SRC将涵盖散装和单细胞磁体，包括整个外显子组测序（WES），散装RNA-Seq，单个 核RNA-Seq和Atac-Seq（SnRNA-Seq和Snatac-Seq），空间转录组学（visium）以及 全球蛋白质组学和磷酸蛋白质组学。对于成像，SRC覆盖了多重目标成像 （codex/chocycler），体内临床前分子和放射学成像以及标准免疫荧光 （如果）染色。 SRC还负责调整项目的不断变化以及介绍 新技术和工具，以协助项目并完成中心的任务。