Selective Control of Synaptically-Connected Circuit Elements by Interluminescence - Diversity Supplement SILVAGNOLI

通过间发光选择性控制突触连接的电路元件 - Diversity Supplement SILVAGNOLI

• 批准号: 10731169
• 负责人: UTE H HOCHGESCHWENDER
• 金额: $ 7.98万
• 依托单位: CENTRAL MICHIGAN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10731169
• 关键词: Acute; Address; Award; BRAIN initiative; Behavior; Brain; Complex; Data; Development; Disease; Education; Electrophysiology (science); Elements; Ensure; Epilepsy; Experimental Models; Fluorescence Microscopy; Fostering; Foundations; Goals; Injections; Memory Loss; Mental Depression; Mental disorders; Mentors; Methods; Mission; Neurosciences; Parents; Public Health; Research; Role; Schizophrenia; Solid; Synaptic plasticity; Therapeutic; Time; Training; United States National Institutes of Health; Validation; Work; addiction; autism spectrum disorder; career; career development; design; experience; experimental study; extracellular; in vivo; innovation; interest; meetings; nervous system disorder; neuroregulation; new technology; novel; parent grant; tool

PROJECT SUMMARY/ABSTRACT The Diversity Supplement candidate will be trained in all aspects of validating Interluminescence in vivo, from stereotaxic injections of AAV, verifying construct expression through fluorescence microscopy, to acute extracellular recordings and computational sophisticated electrophysiological analysis. The experimental approach will allow the candidate to assess Interluminescence within a well-defined experimental model while building a solid conceptual and experimental foundation for his graduate work. The training experiences the candidate will gain are critical for his understanding, designing, and interpretation of state-of-the-art neuroscience experiments. The training plan takes into consideration that the candidate is at the beginning of his graduate training, gives him a ‘hot start’ in his education, and ensures that the candidate will have an exceedingly strong background for meeting his career goals. His training will have ranged from hands- on experience in designing and executing in vivo experiments for controlling neural activity and behavior to evaluating and interpreting the collected data. Therefore, this training in in vivo electrophysiology will not only significantly contribute to the parent grant but will specifically provide him the experience required to pursue his long-term interest in synaptic plasticity and behavior. The proposed training outlined in the application is ideal given the candidate’s early career stage and is tailored to having the greatest impact at the earliest possible time point. An established team training strategy between the two mentors will foster the candidate’s ability to swiftly reach a level where he can independently investigate the role of synaptically-connected circuits. The proposed supplement activities will increase the parent award’s overall impact within the original scope of award while at the same time advancing the training and career development of the candidate.

项目摘要/摘要 多样性补充候选人将在验证体内验光的各个方面进行培训， 立体定位注射AAV，通过荧光显微镜验证构建体表达，急性 细胞外记录和计算复杂的电生理分析。实验 方法将使候选人能够在明确定义的实验模型中评估发光 为他的研究生工作建立坚实的概念和实验基础。 候选人将获得的培训经验对于他的理解，设计和解释至关重要 最先进的神经科学实验。培训计划考虑到候选人在 他的毕业生培训的开始，使他在教育中取得了“热门开始”，并确保候选人将 具有实现他的职业目标的极强背景。他的培训将从手中进行 - 关于设计和执行体内实验的经验，以控制神经活动和行为 评估和解释收集的数据。因此，对体内电生理学的这种培训不仅将 为父母的赠款做出重大贡献，但会特别为他提供追求他的经验 长期对突触可塑性和行为的兴趣。 鉴于候选人的早期职业阶段，该应用程序中概述的拟议培训是理想的选择 在最早可能的时间点上产生最大的影响。建立的团队培训策略 两位导师将培养候选人迅速达到他可以独立调查的水平的能力 突触连接电路的作用。 拟议的补充活动将增加父母奖在原始范围内的总体影响 同时奖励候选人的培训和职业发展。