Structure, Function, and Regulation of GPCRs

GPCR 的结构、功能和调控

• 批准号: 7594699
• 负责人: James F. Battey
• 金额: $ 38.86万
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7594699
• 关键词: Alleles; Biological Assay; California; Chemicals; Code; Collaborations; Complementary DNA; Coupling; Databases; Expressed Sequence Tags; Family; GTP-Binding Proteins; Generations; Genes; Goals; Hand; In Situ Hybridization; In Vitro; Intramural Research; Intramural Research Program; Ion Channel; Laboratories; Libraries; Ligands; Mammals; Messenger RNA; Molecular; Mus; Mutate; Numbers; Pennsylvania; Philadelphia; Property; Protein Subunits; Receptor Cell; Regulation; Research; Role; Sampling; Signal Transduction; Structure; Taste Perception; Testing; Tissues; United States National Institutes of Health; Universities; cDNA Library; interest; member; mouse model; novel; preference; receptor; reconstitution; selective expression; sweet taste perception

This project studies the structure, function, and regulation of mammalian taste receptors and other molecules involved in taste signaling. In collaboration with Susan Sullivan in the NIDCD Division of Intramural Research, we have created a subtracted, normalized mouse cDNA library enriched in genes selectively expressed in taste receptor cells. From this library, we have isolated hundreds of cDNAs from taste receptor cell selective expression, when assayed by in situ hybridization. These cDNAs include members of the sweet and bitter taste receptor families, as well as G protein subunits, ion channels, and other molecules likely to be involved in taste signaling. We are currently using heterologous expression and mouse models to explore the function of these taste-selective molecules. In a collaboration with John Northup's laboratory in the NIDCD Division of Intramural Research, we are using an in vitro receptor reconstitution assay to study the ligand preferences and coupling properties of normal and mutated bitter receptors.

该项目研究哺乳动物味觉受体和其他参与味觉信号传导的分子的结构、功能和调节。我们与 NIDCD 校内研究部的 Susan Sullivan 合作，创建了一个消减、标准化的小鼠 cDNA 文库，其中富含在味觉受体细胞中选择性表达的基因。通过原位杂交分析，我们从这个文库中分离出了数百个来自味觉受体细胞选择性表达的 cDNA。这些 cDNA 包括甜味和苦味受体家族的成员，以及 G 蛋白亚基、离子通道和其他可能参与味觉信号传导的分子。我们目前正在使用异源表达和小鼠模型来探索这些味觉选择性分子的功能。我们与 NIDCD 校内研究部的 John Northup 实验室合作，使用体外受体重构测定来研究正常和突变苦味受体的配体偏好和偶联特性。

项目成果

The knockout mouse project.

• DOI: 10.1038/ng0904-921
• 发表时间: 2004-09
• 期刊: Nature genetics
• 影响因子: 30.8

Stem cells: current challenges and future promise.

干细胞：当前的挑战和未来的希望。

• DOI: 10.1002/dvdy.21203
• 发表时间: 2007
• 期刊: Developmental dynamics : an official publication of the American Association of Anatomists
• 作者: Battey,JamesF

A short primer on stem cells.

关于干细胞的简短引物。

• 发表时间: 2005
• 期刊: Maryland medicine : MM : a publication of MEDCHI, the Maryland State Medical Society
• 作者: Zerhouni,EliasA;BatteyJr,JamesF;Downing,GregoryJ
• 通讯作者: Downing,GregoryJ