LIPID METABOLISM IN SYMBIOTIC COELENTRATES

共生腔肠动物中的脂质代谢

• 批准号: 6271705
• 负责人: James F. Battey
• 金额: $ 3.28万
• 依托单位: UNIVERSITY OF THE VIRGIN ISLANDS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6271705
• 关键词: Cnidaria; algae; alternatives to animals in research; aquatic biology; autoradiography; centrifugation; coral; gas chromatography; gel electrophoresis; lipid biosynthesis; photosynthesis; protein isoforms; protein structure function; reproductive development; scintillation counter; symbiosis; thin layer chromatography; tissue /cell culture; vitellin; western blottings

The broad, long-term objectives of the proposed research are to characterize, quantify and determine the significance of acyl-carrier proteins in the production of lipids by the symbiotic dinoflagellate algae (zooxanthellae) of tropical marine coelenterates. The proposed research will examine one of the most important biochemical interactions in these symbioses: the production of lipid from carbon photosynthetically fixed by zooxanthellae and the utilization of this lipid by the coelenterate host. Symbiotic coelenterates are simple organisms that have been used as model systems to study basic biomedical processes, such as calcification and histocompatibility. Symbiotic coelenterates are also and ideal model system for the study of lipid metabolism. The proposed research will focus on the role of acyl-carrier proteins in the production and export of lipid from zooxanthellae. Specific aims of the research include the following: A) Determination of the levels of free and acylated acyl-carrier proteins and testing the hypothesis that rates of lipid synthesis will correlate with level of ACP in the zooxanthellae. B) Determine if zooxanthellae have single isoforms of ACP like all other known unicellular organisms and test the hypothesis that when in symbiosis zooxanthellae have multiple ACP isoforms. C) Determine if the coelenterate host can control the lipid metabolism of its symbiotic algae and teat the hypotheses that environmental stress and reproductive status alter levels of ACP in zooxanthellae and the metabolic fate of zooxanthellae-derived lipids. This research will increase our general understanding of both lipid metabolism and metabolic interchange in unicellular as well as multicellular organisms.

拟议研究的广泛，长期目标是 表征，量化和确定酰基载体的重要性 共生鞭毛藻藻类生产脂质的蛋白质 （Zooxanthellae）的热带海洋腔。 拟议的研究 将检查其中最重要的生化相互作用之一 共生：由碳光合固定的脂质产生 Zooxanthellae和腔室宿主对这种脂质的利用。 共生卵巢是已被用作模型的简单生物 研究基本生物医学过程的系统，例如钙化和 组织相容性。 共生腔也是理想的模型 研究脂质代谢的系统。 拟议的研究将重点 关于酰基载体蛋白在脂质产生和出口中的作用 来自Zooxanthellae。 该研究的具体目的包括以下内容： a）测定游离和酰基化的酰基载体蛋白的水平 并测试脂质合成速率将相关的假设 在Zooxanthellae中的ACP水平。 b）确定Zooxanthellae是否具有 与所有其他已知的单细胞生物和测试一样，ACP的单一同工型 当在共生的动物Xanthelae中时有多个ACP的假设 同工型。 c）确定腔室宿主是否可以控制脂质 其共生藻类的代谢和乳头的假设 环境压力和生殖状态改变了ACP的水平 Zooxanthellae和Zooxanthellae衍生的脂质的代谢命运。 这 研究将增加我们对这两种脂质代谢的一般理解 以及单细胞和多细胞的代谢互换 有机体。