Harnessing the Power of p53 with Panaxynol from American Ginseng to Suppress Colitis and Prevent Colon Cancer - admin supp

利用 p53 与西洋参中的人参醇的功效来抑制结肠炎和预防结肠癌 - admin support

• 批准号: 10380268
• 负责人: ELIZABETH ANGELA MURPHY
• 金额: $ 2.65万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10380268
• 关键词: American; Anti-Inflammatory Agents; Area; Award; Cancer Model; Carrots - dietary; Celery; Chronic; Colitis; Colon Carcinoma; Complement; Development; Ensure; Etiology; Exhibits; Fennel - dietary; Financial Hardship; Genetic; Ginseng Preparation; Goals; Immune Targeting; Immunologics; Inflammation; Inflammatory Bowel Diseases; Intestines; Lead; Link; Literature; Mentors; Methods; Modeling; Neuroimmune; Neurologic; Neurons; Neuroprotective Agents; Neurosciences Research; Paper; Positioning Attribute; Postdoctoral Fellow; Property; Protocols documentation; Public Health; Publishing; Relapse; Research; Research Institute; Research Training; Resources; Sensory; TP53 gene; Techniques; Testing; Training; Work; afferent nerve; base; career; career development; colon cancer prevention; cost effective; design; gastrointestinal system; high risk; immune function; interest; macrophage; notch protein; parent grant; preservation; research and development; tumor immunology

PROJECT SUMMARY Inflammatory Bowel Disease (IBD) is a chronic condition with a high risk for development of colon cancer. This illness has a high financial burden due to its relapsing and remitting features. Emerging evidence links the intestinal neuro-immune axis to the development and progression of IBD. Both macrophages and sensory afferent nerves are in close proximity in the gastrointestinal system, and they have the machinery necessary to communicate with one another. Thus, we are interested in targeting the immunological and neurological components of IBD and associated colon cancer. Specifically, we want to study the neuro-immune interactions between macrophages and sensory afferent nerves. Panaxynol is a bioactive component of American Ginseng, carrots, celery, and fennel. Panaxynol has been shown to exhibit anti-inflammatory properties and neuroprotective effects, which make it an ideal compound to test our hypothesis that panaxynol can modulate neuro-immune interactions in colitis and colitis-associated colon cancer. Two aims are proposed: 1) To determine the impact of panaxynol on sensory afferent nerves in the gastrointestinal system, and 2) To examine whether panaxynol influences macrophage interactions with sensory nerves. Based on the current literature and our previous work using methodical and scientifically rigorous protocols, we hypothesize that panaxynol can be developed as a safe, cost-effective anti-inflammatory and neuro-protective agent to be used for IBD and associated colon cancer. The studies in this proposal were designed by Gustavo and his Mentors (Drs. Murphy and Velazquez) taking into consideration: 1) previous research and training, 2) need for additional training, 3) research potential, 4) mentors’ commitment to training, 5) available resources, 6) career goals and objectives, and 7) the public health importance of the proposed research. Drs. Murphy (MPI on parent grant and Mentor) and Dr. Velazquez (Mentor) will guide Gustavo through each aspect of the research and training plan. Specifically, Dr. Murphy will provide training in aspects that involve immune function including macrophages and Dr. Velazquez will provide training in aspects involving neuro-protective properties of panaxynol. Both mentors will provide guidance in natural compound (panaxynol) research, and in colitis/cancer models. It also is important to point out that Drs. Murphy and Velazquez are at different stages of their careers so not only will their research areas complement each other but so will their training in professional development. Under this award it is expected that Gustavo will: 1) achieve new training in cutting edge techniques in cancer, immunology, and neuroscience research, 2) publish at least 6 papers with a minimum of 2 as first author, and 3) be successful in attaining a post-doctoral fellow position at a top-notch research institute. The elaborate research and career development training plan that is proposed will ensure that these goals are reached.

项目摘要 炎症性肠病（IBD）是一种慢性疾病，其结肠癌的发展风险很高。这 由于其复发和恢复功能，疾病的财务燃烧很高。新兴证据与 肠道神经免疫轴对IBD的发展和进展。巨噬细胞和感觉 传入的神经在胃肠系统中紧邻，并且它们具有必要的机械 彼此交流。那我们有兴趣针对免疫学和神经系统 IBD和相关结肠癌的成分。具体而言，我们想研究神经免疫相互作用 巨噬细胞和感觉传入神经之间。 Panaxynol是美国的生物活性组成部分 人参，胡萝卜，芹菜和茴香。已证明Panaxynol具有抗炎特性和 神经保护作用，这使其成为测试我们的假设的理想化合物，即Panaxynol可以调节 结肠炎和结肠炎相关结肠癌的神经免疫相互作用。提出了两个目标：1） 确定Panaxynol对胃肠系统中感觉传入神经的影响，2）对 检查Panaxynol是否影响巨噬细胞与感觉神经的相互作用。基于电流 文学和以前使用有条理和科学严格的协议的工作，我们假设 Panaxynol可以作为安全，具有成本效益的抗炎和神经保护剂的开发 对于IBD和相关的结肠癌。 该提案中的研究是由Gustavo及其导师（Murphy和Velazquez博士）设计的 考虑到：1）先前的研究和培训，2）需要额外的培训，3）研究潜力，4） 导师对培训的承诺，5）可用资源，6）职业目标和目标，7）公众 拟议研究的健康重要性。博士。 Murphy（父母Grant and Mentor的MPI）和Velazquez博士 （导师）将指导古斯塔沃（Gustavo）完成研究和培训计划的各个方面。特别是，墨菲博士将 在涉及免疫功能的方面提供培训，包括巨噬细胞和Velazquez博士将提供 涉及Panaxynol神经保护特性方面的培训。两位导师将在 天然化合物（Panaxynol）研究以及结肠炎/癌症模型。指出DRS也很重要。 墨菲（Murphy）和维拉兹克斯（Velazquez）处于职业的不同阶段，因此他们的研究领域不仅会完成 彼此，但他们在专业发展方面的培训也是如此。根据该奖项，预计古斯塔沃 意志：1）在癌症，免疫学和神经科学研究方面的最先进技术方面实现新的培训，2） 至少发表6篇论文，至少有2篇作为第一作者，3）成功参加博士后 一流研究所的职位。精心制作的研究和职业发展培训计划 提出的将确保达到这些目标。