WU-SN-TMC Biospecimen Core

WU-SN-TMC 生物样本核心

• 批准号: 10376525
• 负责人: Ryan C Fields
• 金额: $ 19.2万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10376525
• 关键词: WU; SN; TMC; Biospecimen; Core

Biospecimen Core Summary/Abstract Senescence is a complex phenotype that likely consists of many states that have been implicated in human health and disease. We aim to create the Washington University Senescence Tissue Mapping Center (WU- SN-TMC), which will leverage our expansive infrastructure to generate multi-omics and imaging data to build high resolution, multi-dimensional atlases of cellular senescence across tissue types and lifespan that can be used to enable basic and translational senescence research. Our WU-SN-TMC will develop cellular senescence atlases using 500 human samples for four essential tissue types: bone marrow, breast, colon, and liver. To tackle such an ambitious goal, the WU-SN-TMC Biospecimen Core (WU-SN-TMC BC) has been designed to facilitate the collection of human tissues across a wide range of ages. In addition, the WU-SN- TMC BC will collect multiple tissues from individuals to allow us to compare senescent numbers and states across tissues from a single individual. The WU-SN-TMC BC will accomplish this goal by using sample acquisition pipelines that are divided into four tissue prospective collection protocols (CPs) that leverage WU clinical expertise and patient volume to collect relevant biospecimens. These CPs include: 1) CP1, which will collect bone marrow from normal donors without chronic disease across ages; 2) CP2, which includes normal breast, bone marrow and skin from patients undergoing cosmetic breast surgery across ages; 3) CP3, which includes patients undergoing endoscopy who will provide normal stomach, small bowel, and colon across ages; and 4) CP4 includes patients undergoing laparoscopic upper gastrointestinal surgery that will provide normal bone marrow, liver, and skin, again across ages. While our focus is on senescence during human aging, some patients in CP2 and CP4 will have received prior chemotherapy for cancers in unrelated tissues (i.e. biospecimens will be from unaffected, cancer-free tissues). This cohort will provide the opportunity to compare age-matched controls +/- chemotherapy and allow perturbations tested in mice in the Biological Analysis Core (BAC) to be mapped back to human tissue for relevance, further enriching the bone marrow, breast, colon, and liver atlases we will produce. The existing infrastructure at WU, our patient volume and the expertise of our team will ensure the success of the WU-SN-TMC BC.

Biospecimen核心摘要/摘要 衰老是一种复杂的表型，可能由许多与人类有关的州组成 健康与疾病。我们旨在建立华盛顿大学衰老组织图中心（Wu- SN-TMC），它将利用我们的广泛基础架构来生成多词和成像数据 跨组织类型和寿命的高分辨率，多维图谱可以是 用于实现基本和转化的衰老研究。我们的WU-SN-TMC将发展出细胞 使用500种人类样品的四种基本组织类型的衰老地图酶：骨髓，乳房，结肠， 和肝脏。为了解决这样一个雄心勃勃的目标，WU-SN-TMC Biospecececececececececececececen Core（WU-SN-TMC BC）已经是 旨在促进各种年龄段的人类组织的收集。另外，Wu-sn- TMC BC将从个人那里收集多个组织，以使我们能够比较衰老数量和状态 跨个体的组织。 WU-SN-TMC BC将通过使用样品来实现此目标 采集管道分为四个利用WU的组织预期收集方案（CPS） 临床专业知识和患者量以收集相关的生物测量。这些CP包括：1）CP1，将 从年龄段的没有慢性疾病的正常供体中收集骨髓； 2）CP2，其中包括正常 乳房，骨髓和皮肤，来自年龄段的整容乳房手术的患者； 3）cp3，其中 包括正在进行内窥镜检查的患者，这些患者将提供正常的胃，小肠和结肠 年龄； 4）CP4包括接受腹腔镜上胃肠道手术的患者 正常的骨髓，肝脏和皮肤，再次跨年龄。而我们的重点是人类期间的衰老 衰老，CP2和CP4中的某些患者将接受无关组织中的癌症的化学疗法 （即，生物测量将来自未受影响的无癌组织）。这个队列将为 比较年龄匹配的对照+/-化学疗法，并允许在生物学的小鼠中测试的扰动 分析核心（BAC）将映射回人体组织以获得相关性，进一步丰富了骨髓， 我们将产生乳房，结肠和肝意图酶。 WU的现有基础设施，我们的患者数量和 我们团队的专业知识将确保WU-SN-TMC BC的成功。