Translational Study of Vocal Exercise Dose-response

发声运动剂量反应的转化研究

基本信息

批准号：
9249556
负责人：
Aaron Matthew Johnson
金额：
$ 20.12万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-04-01 至 2020-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9249556
关键词：
Acoustics Affect Aging Animal Model Animals Area Behavioral Clinical Clinical Trials Design Complement Control Groups Dose Elderly Endoscopy Exercise Exercise Physiology Foundations Goals Grant Growth Home environment Human Hypertrophy Intervention K-Series Research Career Programs Kinesiology Laboratories Laryngeal muscle structure Larynx Lead Limb structure Loudness Magnetic Resonance Imaging Measures Mentors Mentorship Morphology Muscle Muscle Fibers Muscle function Muscular Atrophy Neurologic Neuromuscular Junction Operant Conditioning Outcome Outcome Measure Paralysed Peer Review Phonation Phosphorylation Preparation Proteins Publications Rattus Recording of previous events Reporting Research Research Methodology Research Project Grants Resolution Rotation Signal Transduction Speed Structure Testing Therapeutic Intervention Thyroarytenoid Muscle Training Translational Research Translations Ultrasonics Voice Voice Disorders Woman Work Writing aged base career development clinical care design dosage evidence base exercise intervention functional improvement improved innovation kinematics men muscle form muscle hypertrophy muscle strength muscular structure neuromuscular neuromuscular system normal aging novel post intervention primary outcome programs public health relevance resistance exercise response senescence skeletal muscle growth strength training success translational approach translational study vocal cord vocalization voice therapy young adult

项目摘要

DESCRIPTION (provided by applicant): Voice therapy is essential for improving vocal function across a range of voice disorders. For hypofunctional voice disorders associated with aging, voice therapy can improve vocal quality and stamina using vocal exercises that engage the intrinsic laryngeal musculature, but the mechanisms underlying these improvements are unknown. Putative mechanisms are laryngeal muscle growth (hypertrophy) and increased strength based on findings from limb exercise studies that report improvement of these factors along with functional improvements following progressive resistance exercise. Understanding how vocal exercise impacts laryngeal muscle structure and function is critical for determining the most appropriate dose of voice therapy. The overall objective of this research is to determine the dose-response relationship of vocal exercise to both vocal function and laryngeal muscle hypertrophic signaling and growth. Our central hypothesis is that only a high dose of vocal exercise based on progressive resistance training principles will result in beneficial changes in both vocal function and laryngeal muscle hypertrophy in the aging voice. We will test this hypothesis using a translational approach that includes: (1) an innovative behavioral animal model, in which rats will be trained to increase the number of ultrasonic vocalizations, allowing us to investigate neuromuscular mechanisms that are not possible to study in humans, including changes in muscle fiber size and type, neuromuscular junctions, and hypertrophic signaling regulating skeletal muscle growth in response to exercise; and (2) vocal training in humans incorporating traditional clinical measures of vocal function with novel magnetic resonance imaging of laryngeal morphology. This research has two specific aims: (1) To determine the laryngeal hypertrophic neuromuscular response to vocal exercise dosage in young adult and old rats; and (2) To determine the vocal exercise dose-response of laryngeal structure and function in aged men and women. The candidate for this career development award has strong training in the clinical care of voice disorders and in using translational research methods to investigate laryngeal neuromuscular mechanisms. Additional training and mentorship will allow the candidate to develop expertise in muscle exercise physiology and human clinical trial design and implementation. The assembled mentoring team has research expertise in these areas as well as a history of successful and effective mentoring to guide the candidate through the research project described above and the proposed career development activities, including formal coursework in kinesiology, rotations in the mentors' laboratories, and mentored grant writing and peer-review publication preparation. The outcome of this proposal will not only be an understanding of how vocal exercise dose impacts vocal function and structure, but will also help the candidate launch a productive and independent research program to continue investigating laryngeal neuromuscular mechanisms underlying functional changes resulting from voice therapy and vocal training.

描述（由适用提供）：语音疗法对于改善各种语音障碍的人声功能至关重要。对于与衰老相关的功能性语音障碍，语音疗法可以使用与内在的喉部肌肉的声音练习来改善人声质量和耐力，但是这些改进的机制却未知。假定的机制是喉肌的生长（肥大）和根据肢体运动研究的发现提高强度，这些研究报告了这些因素的改善以及渐进性运动后功能改善。了解声带运动如何影响喉肌的结构和功能对于确定最合适的语音疗法至关重要。这项研究的总体目的是确定声乐运动与声带功能和喉部肌肉肥厚的信号传导和生长的剂量反应关系。我们的中心假设是，只有基于渐进式抗性训练原理的高剂量锻炼才会导致声音中的声带和喉部肌肉肥大的有益变化。 We will test this hypothesis using a translational approach that includes: (1) an innovative behavioral animal model, in which rats will be trained to increase the number of ultrasonic vocalizations, allowing us to investigate neuromuscular mechanisms that are not possible to study in humans, including changes in muscle fiber size and type, neuromuscular junctions, and hypertrophic signaling regulation skeletal muscle growth in response to exercise; （2）人类的声带训练，将传统的人声功能临床指标与喉形态的新型磁共振成像结合在一起。这项研究具有两个具体的目的：（1）确定年轻大鼠的喉肥大性神经肌肉反应对人声运动剂量的反应；（2）确定年龄男性和女性的喉结构和功能的声带运动剂量反应。该职业发展奖的候选人对语音障碍的临床护理以及使用翻译研究方法进行了强有力的培训，以调查喉神经肌肉机制。额外的培训和心态将使候选人能够在肌肉运动生理和人类临床试验设计和实施方面发展专业知识。集会的指导团队在这些领域具有研究专业知识，以及成功有效的指导历史，以指导候选人通过上述研究项目以及拟议的职业发展活动，包括运动学的正式课程，导师实验室的轮换，以及指导的赠款写作和同行培训邀请概述的发布。该提案的结果不仅将了解声带剂量如何影响声音功能和结构，而且还将帮助候选人启动一项富有成效的独立研究计划，以继续调查喉神经肌肉机制，从而引起语音治疗和声音训练引起的功能变化。