Expanding Access to Planar Illumination Microscopy in a Neuroimaging Core

扩大神经影像核心中平面照明显微镜的使用范围

基本信息

批准号：
9247855
负责人：
Paul H Taghert
金额：
$ 9.47万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-03-01 至 2018-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9247855
关键词：
Address Bioinformatics Brain Communities Core Facility Coupled Custom Data Data Analyses Elements Ensure Growth and Development function Image Image Analysis Imaging technology Laboratories Light Lighting Maintenance Maps Methods Microscope Microscopy Mission Modernization Monitor National Institute of Neurological Disorders and Stroke Neurons Neurosciences Neurosciences Research Optics Pharmacology Phototoxicity Physiology Research Research Activity Scanning Schedule Series Specialist Speed Techniques Technology Time Training Universities Washington base design experience flexibility fluorescence imaging high throughput technology imaging modality imaging platform imaging system innovation instrument instrumentation nervous system disorder neuroimaging neurophysiology new technology novel public health relevance rapid growth two-photon

项目摘要

DESCRIPTION (provided by applicant): To support pursuit of the NINDS mission, here we propose to introduce a high-impact novel technology for Imaging to the Washington University Neuroscience community. We intend to provide Core Instrumentation to perform Planar Illumination Microscopy (also known as light sheet microscopy) to catalyze new imaging efforts that aim to monitor large-scale neuronal ensembles. The recent Federal announcement of the Brain Activity Map targets neurophysiology for rapid growth and development in the coming decade. One of the most promising techniques for high-throughput neurophysiology is fluorescence imaging using light sheets. Our implementation, Objective-Coupled Planar Illumination (OCPI) microscopy, achieves imaging speeds that are hundreds or thousands of times faster than two-photon microscopy by simultaneously illuminating all pixels in the objective's focal plane, thereby eliminating the need to collect images by scanning one pixel at a time. The method achieves fast imaging simultaneously with high sensitivity and low phototoxicity and is therefore particularly well-suited to long-term recording periods. The custom-built OCPI instrument will be a Core offering within an existing Imaging Center, the Bakewell Neurolmaging Facility. Therefore, this proposal describes a plan and a schedule by which the new OCPI microscope will be introduced, and the oversight we will establish to ensure that its use is made available fairly and broadly. We also emphasize that we have recent experience in precisely these community-based efforts: we successfully introduced earlier version of this instrumentation as a Core facility and now can foresee the need for more access by a larger community of Neuroscientists to a faster and more flexible version. We plan Facility support for OCPI users that includes a facility manager to provide maintenance and training, a data center for image analysis, and a data manager to help with customized data analysis. We provide a series of quantifiable milestones by which to evaluate progress in offering this new high-impact technology.' By the end of the 4-yr period, we anticipate a steady base of at least 20 different users from across the diverse WU Neuroscience community, and a usage rate at or near full-time.

描述（由申请人提供）：为了支持追求Ninds任务，我们在这里建议向华盛顿大学神经科学社区介绍一项高影响力的新技术。我们打算提供核心仪器，以执行平面照明显微镜（也称为轻度显微镜）来催化新的成像工作，以监测大规模的神经元集合。联邦宣布大脑活动图的目标是神经生理学，以实现未来十年的快速增长和发展。高通量神经生理学的最有希望的技术之一是使用轻板进行荧光成像。我们的实施，客观耦合的平面照明（OCPI）显微镜可以通过同时照明目标焦平面中的所有像素，从而比两光子显微镜的成像速度比两光子显微镜快数百或数千倍，从而消除了通过在一次时扫描图像来收集图像的所有像素，从而消除了一个像素。该方法以高灵敏度和低光毒性同时实现快速成像，因此特别适合长期记录期。定制的OCPI仪器将是现有成像中心（Bakewell Neurolmaging设施）中的核心产品。因此，该提案描述了将引入新的OCPI显微镜的计划和时间表，我们将确定确保其使用的监督可以公平，广泛地提供。我们还强调，我们在这些基于社区的努力方面具有最新的经验：我们成功地介绍了该仪器的早期版本作为核心设施，现在可以预见到，更大的神经科学家社区需要更多地访问更快，更灵活的版本。我们计划为OCPI用户提供设施支持，其中包括设施管理器，以提供维护和培训，图像分析数据中心以及数据经理，以帮助进行自定义数据分析。我们提供了一系列可量化的里程碑，以评估提供这项新的高影响力技术的进展。到4年期结束时，我们预计来自不同WU神经科学社区的至少20个不同用户的稳定基础，并且在全日制或接近全日制的使用率。