Animal Core B

动物核心B

基本信息

批准号：
9354252
负责人：
Enrique Cadenas
金额：
$ 37.33万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-08-15 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9354252
关键词：
Age Aging Alleles Alzheimer&apos s Disease Alzheimer&apos s disease risk American Animal Model Animals Autopsy Basic Science Behavior Bioenergetics Biological Birth Blinded Brain Brain Diseases Breeding Cell Culture Techniques Clinical Clinical Research Code Cytology Electrophysiology (science)Enrollment Ensure Female Gene Expression Profiling Genes Goals High Fat Diet Human Image Immunohistochemistry Impaired cognition Inflammatory Irrigation Lead Link Menopause Metabolic Mission Modeling Molecular Profiling Mus Mutation Neurosecretory Systems Online Systems Outcome Ovarian Ovariectomy Pap smear Perimenopause Periodicity Phenotype Population Population Programs Postmenopause Process Progress Reports Randomized Rattus Records Research Risk Risk Factors Rodent Rodent Model Serum Subgroup System Tissue Banks Tissue Sample Tissues Transgenic Mice Translating Vagina Woman aged aging brain animal tissue apolipoprotein E-3 apolipoprotein E-4 brain cell clinical translation data management density endophenotype experience hormone therapy innovation insight microPET middle age phenotypic biomarker prevent programs reproductive senescence sex success

项目摘要

PROJECT SUMMARY-ANIMAL CORE B The mission of our Perimenopause in Brain Aging and Alzheimer's Disease Program Project (P3) is to discover biological transformations in brain that occur during the perimenopausal transition that lead to endophenotypes predictive of risk for Alzheimer's disease (AD). Our goals are to identify the mechanisms by which these transformations occur and to translate these discoveries into strategies to prevent conversion to an at-Alzheimer's-risk phenotype. The proposed program of research builds on our discovery of the perimenopausal bioenergetic transition in brain and its predictive association with cognitive decline in postmenopausal women to investigate the impact of the APOE4 allele on this uniquely female aging experience. Outcomes of our mechanistic to clinical to global population program of research could provide insights into the increased burden of the ApoE4 gene and risk of AD in ApoE4 positive women. The mission of Animal Core (Core B) is to ensure the success of the Perimenopause Program Project through provision of animals as needed to Projects 1–2. To achieve its mission, the Animal Core will extend previously developed rodent models of perimenopause and menopause to humanized ApoE4 and ApoE3 rodent mice and rat models; maintain and track animals from acquisition or birth, determination of cycling status and perimenopausal transition, randomized study enrollment, experimental manipulation, to tissue collection across the entire Perimenopause Program Project; and obtain blinded tissue samples for analyses by Analytic Core and/or Projects.

项目摘要 - 动物核心B 我们围绝经期在大脑衰老和阿尔茨海默氏病计划项目（P3）中的使命是发现在围绝经内过渡期间发生的大脑中的生物转化预测阿尔茨海默氏病风险（AD）的内表型。我们的目标是确定机制通过这些转化并将这些发现转化为防止这些发现的策略转换为At-Alzheimer的风险表型。拟议的研究计划以我们的发现为基础大脑中绝经中的生物能量过渡及其与认知能力下降的预测关联绝经后妇女研究ApoE4等位基因对这种独特女性衰老的影响经验。我们机械到临床到全球研究计划的结果可能会提供洞悉APOE4基因的燃烧增加以及APOE4阳性女性的AD风险。动物核心（核心B）的使命是确保围门计划项目的成功通过根据项目1-2的需要提供动物。为了实现其使命，动物核心将扩展以前开发了围绝经期和更年期的啮齿动物模型至人性化的APOE4和APOE3 啮齿动物小鼠和大鼠模型；维护和跟踪动物的收购或出生，确定骑自行车状态和上绝经跃迁，随机研究入学，实验操作，到组织在整个围栏计划项目中收集；并获得了盲组织样品通过分析核心和/或项目进行分析。