Acquisition of a Q Exactive HF Hybrid Quadrupole-Orbitrap

获取 Q Exactive HF 混合四极杆 Orbitrap

• 批准号: 9273737
• 负责人: KARI B Basso
• 金额: $ 60万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9273737
• 关键词: Anthelmintics; Biosensor; Blood capillaries; Chemistry; Combined Modality Therapy; Communicable Diseases; Complex Mixtures; Core Facility; Data; Dependovirus; Detection; Development; Diagnostic tests; Drug Delivery Systems; Drug Targeting; Funding; Gene Transduction Agent; Goals; HIV drug resistance; HIV-1 protease; Health; Human; Hybrids; Industrialization; Institution; Lipids; Malignant Neoplasms; Mantle Cell Lymphoma; Mass Spectrum Analysis; Measures; Metabolic Diseases; Methods; Multiple Myeloma; Peptides; Pharmacotherapy; Play; Proteins; Proteome; Proteomics; Request for Proposals; Research; Research Project Grants; Resolution; Role; Services; System; Systems Biology; Traumatic Brain Injury; Ubiquitin; Update; aptamer; base; cancer cell; capillary; college; improved; improved outcome; instrument; ion source; mass analyzer; mass spectrometer; metabolomics; multicatalytic endopeptidase complex; nano; nanomaterials; novel therapeutics; personalized medicine; protein complex; tandem mass spectrometry; ultra high resolution; Anthelmintics; Biosensor; Blood capillaries; Chemistry; Combined Modality Therapy; Communicable Diseases; Complex Mixtures; Core Facility; Data; Dependovirus; Detection; Development; Diagnostic tests; Drug Delivery Systems; Drug Targeting; Funding; Gene Transduction Agent; Goals; HIV drug resistance; HIV-1 protease; Health; Human; Hybrids; Industrialization; Institution; Lipids; Malignant Neoplasms; Mantle Cell Lymphoma; Mass Spectrum Analysis; Measures; Metabolic Diseases; Methods; Multiple Myeloma; Peptides; Pharmacotherapy; Play; Proteins; Proteome; Proteomics; Request for Proposals; Research; Research Project Grants; Resolution; Role; Services; System; Systems Biology; Traumatic Brain Injury; Ubiquitin; Update; aptamer; base; cancer cell; capillary; college; improved; improved outcome; instrument; ion source; mass analyzer; mass spectrometer; metabolomics; multicatalytic endopeptidase complex; nano; nanomaterials; novel therapeutics; personalized medicine; protein complex; tandem mass spectrometry; ultra high resolution

ABSTRACT We are resubmitting an improved and updated proposal requesting funds for the purchase of a Thermo Scientific Q Exactive HF Orbitrap LC-MS/MS system to be operated in the Mass Spectrometry Services (MSS) core facility in the Department of Chemistry. The MSS serves more than 90 research groups on the UF campus from twelve departments and colleges. More than 30 non-UF academic institutions and industrial companies also utilize the MSS facility. We are requesting this instrument because its capabilities are well suited for diverse range of research projects including large- and small-biomolecules, characterization of unknowns, and quantitative data on a single platform. The Q Exactive Plus LC-MS/MS is a tandem mass spectrometer that combines a quadrupole mass analyzer for precursor selection with a high-resolution/high mass accuracy Orbitrap mass analyzer. The complete system will include the optional Protein Mode for enhanced analysis of intact proteins and protein complexes, an UltiMate 3000 RSLCnano LC system, a Nanospray Flex Ion Source and tubing required to change the system between capillary and nano flow, Biopharma Finder 1.0, and Proteome Discoverer 2.1 Base and Quantitation. The fields of metabolomics, proteomics, and lipidomics are now recognized as playing a critical role in systems biology and are becoming the focus of the development of diagnostic tests, novel drug therapies, personalized medicine, and understanding of infectious diseases, cancer, and metabolic disorders. The proposed Q Exactive HF Orbitrap system will be used for measuring complex mixtures of proteins, peptides, lipids, metabolites and other biomolecules requiring ultra-high resolution tandem mass spectrometry for detection, identification, and quantitation of those complex mixtures. This data will in turn improve the outcomes of the health related goals of the projects assembled in this proposal, examples including: understanding traumatic brain injury; understanding the ubiquitin- proteasome in the treatment of human cancers including multiple myeloma and mantle cell lymphoma; understanding drug resistance to HIV-1 protease; studying Adeno-associated virus (AAV) as a delivery vehicle or vector for gene therapy; developing biosensors, bioseparation methods, targeted drug delivery, and cancer cell treatment by combining aptamers and nanomaterials; and developing and understanding the mechanism of anthelmintics.

抽象的 我们正在重新提交一项改进和更新的建议，要求资金购买 Thermo Scientific Q Extive HF Orbitrap LC-MS/MS系统将在质量中运行 化学系的光谱服务（MSS）核心设施。 MSS服务更多 来自十二个系和大学的UF校园的90个研究小组。超过30 非UF学术机构和工业公司也利用MSS工厂。我们是 请求此工具，因为它的功能非常适合各种各样的研究 包括大生物分子和小分子的项目，未知数的表征和定量数据 在一个平台上。 Q Extrive Plus LC-MS/MS是串联质谱仪，结合了四极质量质量 使用高分辨率/高质量精度轨道质量分析仪进行前体选择的分析仪。 完整的系统将包括可选的蛋白质模式，以增强完整蛋白的分析 和蛋白质复合物，最终3000 RSLCNANO LC系统，纳米喷离子源和 更改毛细管和纳米流量之间的系统所需的管道，生物制药查找器1.0和 蛋白质组发现者2.1基础和定量。 代谢组学，蛋白质组学和脂肪组学领域现在被认为是在 系统生物学，并正在成为开发诊断测试的重点，新型药物 疗法，个性化医学以及对传染病，癌症和代谢的理解 疾病。提出的Q精确HF Orbitrap系统将用于测量复杂的混合物 蛋白质，肽，脂质，代谢产物和其他需要超高分辨率串联的生物分子 用于检测，鉴定和定量这些复杂混合物的质谱法。这个数据 反过 建议，包括：了解创伤性脑损伤；了解泛素 - 蛋白酶体在治疗包括多发性骨髓瘤和地幔细胞在内的人类癌症治疗中 淋巴瘤；了解对HIV-1蛋白酶的耐药性；研究腺相关病毒（AAV） 作为基因治疗的递送工具或载体；开发生物传感器，生物序列方法， 通过结合适体和纳米材料来靶向药物递送和癌细胞治疗；和 发展和理解驱虫药的机制。