Acquisition of timsTOF Pro for Complex Lipidomic Analyses

购买 timsTOF Pro 用于复杂脂质组学分析

• 批准号: 10413379
• 负责人: KARI B Basso
• 金额: $ 65.84万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10413379
• 关键词: Abdominal Aortic Aneurysm; Aging; Animals; Anthelmintics; Antibiotics; Biology; Communicable Diseases; Communities; Complex; Complex Mixtures; Data; Databases; Detection; Development; Diagnostic tests; Disease; Education; Environmental Risk Factor; Florida; Funding; G-Protein-Coupled Receptors; Genetic; Goals; Gram-Negative Bacteria; Health; Industrialization; Institution; Ions; Libraries; Lipids; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Measures; Membrane Lipids; Metabolic Diseases; Multi-Drug Resistance; Pharmacotherapy; Play; Process; Proteomics; Request for Proposals; Research; Research Personnel; Research Project Grants; Role; Source; Spectrometry; Superbug; System; Systems Biology; United States National Institutes of Health; Universities; college; data quality; improved outcome; instrument; ion mobility; lipidomics; metabolomics; novel therapeutics; personalized medicine; programs; time of flight mass spectrometry; ultra high resolution

ABSTRACT The Mass Spectrometry Research and Education Center (MSREC) at the University of Florida is submitting a new proposal requesting funds for the purchase of a Bruker timsTOF Pro Trapped Ion Mobility Spectrometry Quadrupole Time-of-Flight Mass Spectrometry system to be installed and operated in the MSREC at the University of Florida. We are requesting this instrument specifically for the “4D-Lipidomicsä” analysis of complex lipids for a variety of research programs. This Bruker timsTOF Pro LC-MS will add ultra-high-resolution trapped ion mobility (TIMS) with PASEF MS/MS capabilities to UF NIH-funded investigators that currently is unavailable in the Southeast region and the North/central region of Florida. MSREC serves more than 150 research groups on the UF campus from more than 25 departments, centers, and colleges. More than 30 non-UF academic institutions and industrial companies also frequently utilize the MSREC core lab. The complete system will include the timsTOF Pro LC-IM-MS/MS system with measurements in both MS and Parallel Accumulation Serial Fragmentation (PASEF), an UltiMate 3000 RSLCnano LC system, Apollo II ion funnel ESI source, SimLipid Enterprise Edition Academic, upgrade to Compass Metaboscape 2021 from currently licensed version 5.0, Library MS-MetaboBase 3.0 and Wiley Metlin Mass Spectral Database. The fields of metabolomics, proteomics, and lipidomics are now recognized as playing a critical role in systems biology and are becoming the focus of the development of diagnostic tests, novel drug therapies, personalized medicine, and understanding of infectious diseases, cancer, and metabolic disorders. The proposed timsTOF Pro system will be used for measuring complex mixtures of lipids requiring ultra-high-resolution ion mobility mass spectrometry for detection, identification, and quantitation of those complex mixtures. This data will in turn improve the outcomes of the health related goals of the projects assembled in this proposal, examples including: development of novel therapeutics for cancer and other diseases; fundamental biology of the interactions between genetic factors and environmental factors in the process of animal aging; studying multidrug-resistant Gram- negative bacteria “superbugs” and antibiotics; the relationship between SPMs to abdominal aortic aneurysms; understanding the structural mechanisms by which membrane lipids regulate GPCR function; and developing and understanding the mechanism of anthelmintics. While the requested instruments capabilities are targeted to the needs of the users and health-related goals of the research projects listed herein, the instrument will also provide substantial improvements in data quality and throughput for the community of system biology researchers at the UF.

抽象的 佛罗里达大学的质谱研究与教育中心（孟加拉国）是 提交了一项新提案，要求资金购买Bruker Timstof Pro被困离子 移动光谱法四极杆飞机时间质谱系统要安装，并且 在佛罗里达大学的苏联经营。我们正在专门要求此工具 用于各种研究计划的复杂脂质的“ 4D-脂肪粒”分析。这个布鲁克 timstof Pro LC-MS将使用Pasef MS/MS添加超高分辨率的离子移动性（TIMS） 美国国立卫生研究院资助的调查员的能力，目前在东南地区和 佛罗里达州北/中部地区。 MSREC在UF校园内为150多个研究小组提供服务 来自25个以上的部门，中心和大学。超过30个非UF学术机构和 工业公司还经常利用MSREC核心实验室。 完整的系统将包括Pro LC-IM-MS/MS系统的TIMSTOF，两者都测量 MS和平行积累序列碎片（Pasef），最终3000 RSLCNANO LC系统， Apollo II离子漏斗ESI来源，Simlipid Enterprise Edition Academic，升级到指南针 当前有执照的5.0版，图书馆MS-Metabobase 3.0和Wiley Metlin的代谢2021 质谱数据库。 代谢组学，蛋白质组学和脂肪组学领域现在被认为是在 系统生物学，并正在成为开发诊断测试的重点，新型药物 疗法，个性化医学以及对传染病，癌症和代谢的理解 疾病。提出的timstof Pro系统将用于测量脂质的复杂混合物 需要超高分辨率的离子迁移率质谱来检测，识别和 这些复杂混合物的定量。这些数据反过来将改善与健康相关的结果 该提案中组装的项目的目标，包括：开发新颖的示例 治疗癌症和其他疾病；遗传之间相互作用的基本生物学 动物衰老过程中的因素和环境因素；研究多药耐药的革兰 阴性细菌“超级细菌”和抗生素； SPM与腹主动脉之间的关系 动脉瘤；了解膜脂质调节GPCR功能的结构机制；和 发展和理解驱虫药的机制。 虽然要求的仪器功能针对用户的需求和与健康有关 本文列出的研究项目的目标，该工具还将提供实质性的改进 UF的系统生物学研究人员社区的数据质量和吞吐量。