Flow Cytometry Core

流式细胞术核心

• 批准号: 7701550
• 负责人: ANUJA MATHEW
• 金额: $ 22.05万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7701550
• 关键词: Antibodies; Arts; Categories; Cell Separation; Cell physiology; Cells; Cellular Immunity; Center for Translational Science Activities; Clinical; Data Collection; Elements; Flow Cytometry; Human; Immunology; Laboratory Scientists; Measurement; Phenotype; Research; Research Personnel; Research Project Grants; Sample Size; Sampling; Services; Sorting - Cell Movement; Staining method; Stains; T-Lymphocyte; Techniques; Training; Translational Research; Virus; Virus Diseases; biodefense; design; high throughput analysis; research study; response; technology development; tool

Flow cytometry has become an essential tool for investigators requiring high throughput analysis of single cells. Assessment of the phenotype and function of human T cells during and after a viral infection is a critical element to all CTRHIB projects (Research Projects 1-4 and Technology Development Project 1). The CTRHIB Flow Cytometry Core will provide flow cytometry services to support human immunology research by CTRHIB investigators. The Flow Cytometry Core will provide CTRHIB investigators with access to a state-of-the-art FACS ARIA flow cytometer to permit analysis and sorting of cells. Currently we are able to use 11 different flurochromes on a single sample thus allowing assessment of multiple parameters on a single cell. The core will provide training for scientists and laboratory staff to operate the FACS ARIA for data collection and analysis. It will also provide guidance in design and analysis of flow cytometry experiments, including techniques for cell staining, cell sorting and selection of antibody panels.

流式细胞仪已成为需要对单个单一进行高通量分析的研究人员的重要工具 细胞。评估病毒感染期间和之后人类T细胞的表型和功能是一种 所有CTRHIB项目的关键要素（研究项目1-4和技术开发项目1）。这 Ctrhib流式细胞仪核心将提供流式细胞仪服务以支持人类免疫学研究 由CTRHIB研究人员。流式细胞仪核心将为CTRHIB研究人员提供进入 最先进的FACS ARIA流式细胞仪可允许细胞分析和分类。目前我们能够 在单个样本上使用11种不同的flurochromes，从而评估A上的多个参数 单细胞。核心将为科学家和实验室工作人员提供培训，以操作FACS ARIA以获取数据 收集和分析。它还将在流式细胞仪实验的设计和分析中提供指导， 包括用于细胞染色的技术，细胞分选和抗体面板的选择。