Mouse Core

鼠标核心

• 批准号: 7675873
• 负责人: Christopher B. Wilson
• 金额: $ 32.55万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-20 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7675873
• 关键词: Adjuvant; Affect; Alkaline Phosphatase; Breeding; Budgets; Burkholderia; Centers of Research Excellence; Communicable Diseases; Congenic Mice; Emerging Communicable Diseases; Funding; Generations; Genes; Genetic; Genotype; Human; Idaho; Immune; Immunity; Infection; Intervention; Intestines; Lipid A; Modeling; Mus; Mutant Strains Mice; Natural Immunity; Oregon; Pathogenesis; Plague; Pneumonia; Research Personnel; Resources; Role; Speed; Therapeutic Effect; Transgenic Mice; Universities; Variant; Washington; acquired immunity; biodefense; congenic; mouse model; mutant; pathogen; prophylactic; response

The overall objective of this core is to generate, breed, genotype and provide genetically modified mice to investigators of the NWRCE. The unique resources developed and managed by this core and the increased needs and anticipated utilization of these resources motivated the creation of a separate transgenic mouse core for this competitive renewal. The Specific Aims of this core are: Aim 1) Advise investigators of the RCE on appropriate use of mouse models for their studies. Aim 2) Breeding, husbandry, and genotyping of existing mutant mouse lines and generation of new compound genetic mutants through intercrossing and genotyping of existing mutant mouse lines. Aim 3) Generation of new genetically modified mice. Aim 4) Rapid generation of congenic mice from mice on mixed genetic backgrounds using a 'speed congenics' approach.

该核心的总体目的是生成，繁殖，基因型，并提供转基因的小鼠至 NWRCE的调查员。由该核心开发和管理的独特资源以及增加 这些资源的需求和预期利用促使创建单独的转基因鼠标 这种竞争更新的核心。该核心的具体目的是： 目标1）就适当使用小鼠模型进行研究的研究人员建议研究人员。 目标2）现有突变鼠标线的繁殖，饲养和基因分型以及新的新产生 通过跨和基因分型的现有突变小鼠系的跨和基因分型的复合基因突变体。 目标3）生成新的转基因小鼠。 目标4）使用速度从混合遗传背景上的小鼠快速生成先天小鼠 兴趣的方法。