Benchtop Device for Detection of BoNT in Clinical Samples

用于检测临床样品中 BoNT 的台式设备

• 批准号: 7675190
• 负责人: DOBRIN NEDELKOV
• 金额: $ 24.76万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7675190
• 关键词: Adopted; Biological; Biological Assay; Bontoxilysin; Botulinum Toxins; Calibration; Cities; Clinical; Complex; Detection; Devices; Emerging Communicable Diseases; Food; Hour; Infectious Diseases Research; Liquid substance; Optics; Organ; Peptide Hydrolases; Process; Readiness; Reading; Robot; Sampling; Serum; Solid; System; Systems Integration; Technology; Testing; base; biodefense; biothreat; cost; improved; instrumentation; prototype

We are proposing to develop devices for the quantitative detection of botulinum neurotoxins (BoNT) at attomolar sensitivity with fully automated bench-top instrumentation. We will adopt the existing bead-based BoNT ALISSA technology, developed by the Kalkum lab at City of Hope, into a monolithic microcolumn format to be utilized by common liquid handling robots. We will also develop an automated, highly sensitive, and inexpensive wave-guide-based optical read-out system for integration in the robots. The resulting functional prototype system is expected to automatically process and analyze multiple samples simultaneously and repeatedly (up to 96 per hour). Automated calibration routines and controls for botulinum toxin and non-botulinum toxin proteases will be included. Our assay system will be tested and validated with complex biological matrices such as serum, solid organ and food extracts as well as clinical samples.

我们建议开发用于定量检测肉毒杆菌毒素（BONT）的设备 具有全自动台面仪器的adlol敏感性。我们将采用现有的基于珠的现有 BONT ALISSA Technology由Hope的Kalkum Lab开发，成为一个单片的Microcolumn 普通液体处理机器人使用的格式。我们还将开发自动化，高度敏感的 和廉价的基于波为机器人集成的光学读出系统。结果 功能原型系统有望自动处理和分析多个样品 同时又反复（每小时96个）。肉毒杆菌的自动校准例程和控件 毒素和非肉毒杆菌毒素蛋白酶将包括在内。我们的测定系统将经过测试和验证 复杂的生物学基质，例如血清，固体器官和食物提取物以及临床样品。