ULTRA-VIOLET REFRACTOMETRY OF LIVE CELLS FOR QUANTITATIVE DNA ANALYSIS

用于 DNA 定量分析的活细胞紫外折光法

• 批准号: 8364150
• 负责人: Dax Fu
• 金额: $ 2.23万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364150
• 关键词: Biomedical Research; Cells; DNA; DNA analysis; Funding; Grant; Image; Lasers; Life; Light; Maps; Microscopy; National Center for Research Resources; Organelles; Phase; Ploidies; Principal Investigator; Proteins; Refractive Indices; Refractometry; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; Variant; Viola; absorption; base; cost; ultraviolet

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Interferometric phase microscopy has been widely applied to living cell studies due to its inherent ability to map refractive index variation without exogenous contrast. However, in general these variations are rather weak and does not provide a strong contrast in differentiation of various organelles, but instead only provide their overall biomolecular content. In order to increase refractive index based contrast, we moved into the ultraviolet region of the light spectrum, where protein and DNA show substantial difference in absorption. Based on Kramers-Kronig relationship, we expect that their refractive index dispersion will also be markedly different and therefore can be utilized separate quantification of cellular protein and DNA content in live cell quantitative phase imaging.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 干涉阶段显微镜已被广泛应用于活细胞研究，因为其固有的绘制折射率变化而没有外源对比度的能力。 但是，总的来说，这些变化相当薄弱，并且在分化各种细胞器的情况下并没有提供强烈的对比，而仅提供其整体生物分子含量。为了增加基于折射率的对比度，我们进入了光谱的紫外线区域，蛋白质和DNA在吸收中显示出很大的差异。基于Kramers-Kronig关系，我们希望它们的折射率分散剂也将明显不同，因此可以在活细胞定量相成像中单独使用细胞蛋白和DNA含量的定量。