ULTRA-VIOLET REFRACTOMETRY OF LIVE CELLS FOR QUANTITATIVE DNA ANALYSIS

用于 DNA 定量分析的活细胞紫外折光法

• 批准号: 8170407
• 负责人: Dax Fu
• 金额: $ 2.85万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170407
• 关键词: Cells; Computer Retrieval of Information on Scientific Projects Database; DNA; Funding; Grant; Image; Institution; Life; Light; Maps; Microscopy; Organelles; Phase; Ploidies; Proteins; Refractive Indices; Refractometry; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Variant; Viola; absorption; base; ultraviolet

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Interferometric phase microscopy has been widely applied to living cell studies due to its inherent ability to map refractive index variation without exogenous contrast. However, in general these variations are rather weak and does not provide a strong contrast in differentiation of various organelles, but instead only provide their overall biomolecular content. In order to increase refractive index based contrast, we moved into the ultraviolet region of the light spectrum, where protein and DNA show substantial difference in absorption. Based on Kramers-Kronig relationship, we expect that their refractive index dispersion will also be markedly different and therefore can be utilized separate quantification of cellular protein and DNA content in live cell quantitative phase imaging.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 干涉阶段显微镜已被广泛应用于活细胞研究，因为其固有的绘制折射率变化而没有外源对比度的能力。 但是，总的来说，这些变化相当薄弱，并且在分化各种细胞器的情况下并没有提供强烈的对比，而仅提供其整体生物分子含量。为了增加基于折射率的对比度，我们进入了光谱的紫外线区域，蛋白质和DNA在吸收中显示出很大的差异。基于Kramers-Kronig关系，我们希望它们的折射率分散剂也将明显不同，因此可以在活细胞定量相成像中单独使用细胞蛋白和DNA含量的定量。