Pilot Projects Component (Pilot Proj 2: Guccione)

试点项目组件（试点项目 2：Guccione）

• 批准号: 7657168
• 负责人: Ann Arvin
• 金额: $ 11.82万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7657168
• 关键词: Behavior; Biological Process; Cell physiology; Classification; Clinical Protocols; DNA Microarray Chip; DNA Microarray format; Data; Disease; Enrollment; Event; Gene Expression; Genes; Genome; Human; Imagery; Immune response; Immunization; Individual; Knowledge; Laboratories; Lesion; Life; Maps; Microarray Analysis; Modeling; Monkeys; Pathogenesis; Pattern; Pilot Projects; Smallpox; Smallpox Viruses; Surrogate Markers; Vaccinated; Vaccination; Vaccines; Vaccinia; Vaccinia Vaccine; Vaccinia virus; Viral; cDNA Arrays; cohort; design; emergency service responder; human data; insight; nonhuman primate; programs; research study; response

lot project 2a. Transcriptional microarray analysis of human responses to smallpox vaccination. In the component of this project to be done in the Relman laboratory, we will use cDNA microarrays to characterize global gene expression patterns in response to smallpox vaccination in a pilot cohort of vaccinees enrolled in approved clinical protocols to evaluate vaccinia vaccines or who are being vaccinated as 'first responders'. The value of systematic studies of global gene expression programs is now well established in providing new insights into normal cellular physiology and disease pathogenesis. DNA microarrays provide a rapid and efficient way to build a detailed map of the gene expression program underlying a biological process, even in the absence of well-developed prior knowledge or models of its behavior. These experiments will allow us to build a comprehensive picture, across thousands of genes, of the human response to smallpox vaccination. These patterns can later be used to assess adverse vaccination events, and develop new surrogate markers for a protective vaccine response that does not rely on visualization of a Jennerian lesion. Although beyond the scope of this proposal, these data will create a framework within which to compare protective and non-protective vaccine responses in a non-human primate model of smallpox, and derive patterns of protection from the human data using data from monkeys challenged with live smallpox virus. Our long-term objective is to use genome-wide expression patterns as a means of predicting protective vaccine responses against smallpox, as well as adverse.responses to vaccine, in humans early after immunization. Our Specific Aims follow from this background and rationale.

地段项目 2a。人类对天花反应的转录微阵列分析 疫苗接种。 在该项目的 Relman 实验室完成的部分中，我们将使用 cDNA 微阵列来表征响应天花疫苗接种的全局基因表达模式 参加经批准的临床方案以评估痘苗疫苗的试点接种者队列或正在接种疫苗的接种者 作为“第一响应者”接种疫苗。全球基因表达系统研究的价值 项目现已在提供对正常细胞生理学和疾病的新见解方面得到了很好的发展 发病。 DNA 微阵列提供了一种快速有效的方法来构建详细的基因图谱 表达程序是生物过程的基础，即使在没有充分开发的先验条件的情况下 其行为的知识或模型。这些实验将使我们能够建立一个全面的图景， 跨越数千个基因，研究人类对天花疫苗接种的反应。这些模式稍后可以 用于评估不良疫苗接种事件，并开发保护性疫苗的新替代标记 不依赖于詹纳病变可视化的反应。虽然超出了这个范围 根据提案，这些数据将创建一个框架，在其中比较保护性和非保护性 非人灵长类天花模型中的疫苗反应，并从天花中得出保护模式 人类数据使用来自受到活天花病毒攻击的猴子的数据。我们的长期目标是 使用全基因组表达模式作为预测保护性疫苗反应的手段 免疫后早期人类的天花以及对疫苗的不良反应。我们的具体 目标是从这个背景和基本原理出发的。