Dynamics of Hair Bundle Proteins

发束蛋白的动力学

基本信息

批准号：
8039124
负责人：
Jung-Bum Shin
金额：
$ 24.08万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-01-11 至 2012-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8039124
关键词：
Address Affect Age Aging Americas Amino Acids Arginine Auditory Basilar Papilla Chickens Chimeric Proteins Clinical Digestion Equilibrium Generations Goals Hair Hair Cells Health Hearing Human Hydrogen Peroxide Image Imaging technology Immunoprecipitation Label Lead Life Link Mass Spectrum Analysis Measures Mechanics Mediating Methionine Methods Molecular Monitor Natural regeneration Noise Organ Organ Culture Techniques Oxidative Stress Pathway interactions Post-Translational Protein Processing Process Proteins Proteomics Protocols documentation Radioactive Radioactivity Rana Rattus Resolution Saccule structure Sampling Sensory Hair Stable Isotope Labeling Stimulus Stress Techniques Transgenic Organisms Ubiquitination Validation Xenopus age related cellular imaging disability hearing impairment in vivo innovation interest multicatalytic endopeptidase complex prevent protein complex protein degradation repaired research study response

项目摘要

Hair cell mechanotransduction is the process that underlies our senses of hearing and balance. The focus of the Shin lab is the study of proteins that mediate the exquisitely sensitive mechanotransduction process. Stress factors such as reactive radicals and mechanical overstimulation can damage hair cell proteins, raising the question how these proteins are repaired or replaced. Our hypothesis is that the main strategy for maintaining integrity of hair ceil proteins is a constant turnover of proteins. I am therefore interested in the dynamic turnover of hair ceil proteins, especially in response to harmful impacts such as oxidative stress and noise damage. The goal of my present ROO proposal is to use mass spectrometry analysis of metabolically labeled proteins and in vivo imaging of hair bundle proteins to study turnover and dynamics of hair cell proteins. Lay summary: The health of the hearing organ depends on the constant replacement of its molecular components. The proposed study uses mass spectrometry and imaging technologies to address the question whether harmful environmental stimuli such as noise damage can influence the turnover of proteins important for hearing. The results of this study wiii help to develop methods to prevent and restore hearing losses in humans.

毛细胞机械转导的过程是我们听力和平衡感的基础。重点 Shin Lab是介导精确敏感的机械转导过程的蛋白质的研究。反应性自由基和机械过度刺激等应力因素会损害毛细胞蛋白，提出了如何修复或更换这些蛋白质的问题。我们的假设是保持头发CEIL蛋白的完整性是蛋白质的持续营业额。因此，我对头发CEIL蛋白的动态营业额，尤其是为了响应有害影响，例如氧化应激和噪声损坏。我目前的ROO提案的目的是使用代谢的质谱分析标记的蛋白质和头发束蛋白的体内成像，以研究毛细细胞的营业额和动力学蛋白质。摘要摘要：听力器官的健康取决于其分子的恒定更换成分。拟议的研究使用质谱和成像技术来解决疑问有害环境刺激（例如噪声损伤）是否会影响蛋白质的周转率听力很重要。这项研究的结果WIII有助于开发预防和恢复听力的方法人类的损失。