Advanced Single Cell Technology Innovation Core

先进单细胞技术创新核心

基本信息

批准号：
10729389
负责人：
Peter Alan Sims
金额：
$ 14.25万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-19 至 2028-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10729389
关键词：
ATAC-seq Address Apoptosis Bar Codes CRISPR interference Cancer Center Cell Communication Cell Death Cell Survival Cell model Cells Chromatin Clustered Regularly Interspaced Short Palindromic Repeats Computer Models Cytometry Epitopes Gene Expression Gene Expression Profile Gene Expression Profiling Gene Pool Genomic Library Genomics Goals Guide RNA Human Image Immunofluorescence Immunologic In Situ Individual Joints Libraries Link Malignant Neoplasms Messenger RNA Methods Microfluidics Molecular Molecular Analysis Molecular Target Mus Periodicity Pharmaceutical Preparations Preparation Proteins Proteomics Protocols documentation Quality Control RNA Research Personnel Resolution Sampling Slice Solid Neoplasm System Technology Therapeutic Tissues Transcript Validation analytical method cell type cellular imaging droplet sequencing experimental analysis live cell imaging microfluidic technology multiple omics posttranscriptional response single cell analysis single cell technology single-cell RNA sequencing technological innovation tool transcriptome sequencing tumor tumor microenvironment

项目摘要

PROJECT SUMMARY The proposed Cancer Systems Therapeutics (CaST) Center focuses on understanding and targeting molecularly distinct yet co-existing cell states and transitions that establish and maintain tumors and tumor microenvironments at a systems level. These goals demand analytical methods with single-cell resolution. The Advanced Single Cell Technology Innovation Core (ASCeTIC) will provide state-of-the-art technology for single- cell genomic analysis, multiplexed single-cell molecular perturbation, and in situ validation to CaST investigators. We will offer a suite of tools including multi-omic methods for joint single-cell RNA/ATAC-seq, single-cell imaging/RNA-seq (SCOPE-seq), and single-cell CRISPRi/RNA-seq (CROP-seq). Recognizing the importance of spatial profiling for both in situ validation and studies of cell-cell interactions in the tumor microenvironment, we will also offer immunohistochemical analysis, RNA-FISH, and cyclic immunofluorescence (cycIF), which can simultaneously quantify tens of protein epitopes in individual cells in a single tissue section.

项目概要拟建的癌症系统治疗 (CaST) 中心侧重于理解和靶向分子治疗建立和维持肿瘤和肿瘤的不同但共存的细胞状态和转变系统级别的微环境。这些目标需要具有单细胞分辨率的分析方法。这先进单细胞技术创新核心（ASCeTIC）将为单细胞提供最先进的技术细胞基因组分析、多重单细胞分子扰动以及 CaST 研究人员的原位验证。我们将提供一套工具，包括用于联合单细胞 RNA/ATAC-seq、单细胞成像/RNA-seq (SCOPE-seq) 和单细胞 CRISPRi/RNA-seq (CROP-seq)。认识到重要性用于肿瘤微环境中细胞间相互作用的原位验证和研究的空间分析，我们还将提供免疫组织化学分析、RNA-FISH 和循环免疫荧光 (cycIF)，这可以同时量化单个组织切片中单个细胞中的数十个蛋白质表位。