Histology and Clinical Repository Core

组织学和临床存储库核心

基本信息

批准号：
7675594
负责人：
JOHN W DAY
金额：
$ 19.17万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-01 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7675594
关键词：
Actins Aftercare Animal Model Arts Biochemical Biological Biological Models Biology Biopsy Caring Catchment Area Cell Lineage Cells Child Chloride Channels Clinic Clinical Communities Comparative Study Consent Country Coupled Cultured Cells DNA DNA Databases Databases Development Diagnostic Diagnostic Procedure Diagnostic tests Disease Enrollment Equus caballus Family Fibroblasts Freezing Funding Generations Genetic Genetic Databases Goals Hereditary Disease Histological Techniques Histology Human Image Laboratories Letters Light Medical center Medicine Methods Microscopic Minnesota Modeling Mus Muscle Muscle function Muscular Dystrophies Musculoskeletal Myoblasts Myopathy Myosin ATPase Myotonic Disorders Myotonic Dystrophy National Institute of Arthritis and Musculoskeletal and Skin Diseases Patients Population Proteins RNA Research Research Ethics Committees Research Methodology Research Personnel Research Project Grants Research Support Residual state Resources Sampling Skin Specific qualifier value Specimen Staining method Stains Tissues Universities Update experience induced pluripotent stem cell lymphoblastoid cell line medical specialties member muscular structure novel programs repository tissue/cell culture

项目摘要

Minnesota CCMBM Core B: Muscle Histology and Clinical Repository Core The University of Minnesota Muscular Dystrophy Clinic cares for one of the largest groups of muscular dystrophy patients in the country. Coupled with the comprehensive genetic and histological diagnostic methods developed and maintained by the MDClinic, this population provides a valuable resource for the MDCenter and this NIAMS Core Center for Musculoskeletal Biology and Medicine. As part of its comprehensive clinical diagnostic facility, the MDClinic has established a repository of DNA, fibroblasts, myoblasts, lymphoblastoid cell lines and residual muscle biopsy tissue from subjects with muscle diseases; if specifically supported for research purposes, this Core will provide great value to many currently funded MDCenter research programs, and will be a valuable asset to the greater MD Research Community. Specific Aims of this Core are: 1) Expand the Muscular Dystrophy Database and DNA/Cell Culture/Tissue Repository. Formally establishing this as a research core will facilitate acquisition and distribution of many more samples. 2) To make state-of-the-art histological techniques accessible to fully and complementarity characterize muscle from patients and from animals that model human muscle disease. These methods will allow detailed characterization of diseased muscle as well as comparative studies after treatment in patients and model systems. 3) To provide tissue or cultured cells for detailed analysis of muscle structure and function. MDCenter research on clinically defined specimens will be of value to many basic and translational programs. Specifically, fibroblasts can be studied directly, differentiated into myoblasts for a full range of genetic, biochemical and force generation (Core C) or used to generate IPS cells (Pilot 1) for developmental studies along multiple cell lineages in specified genetic disorder

明尼苏达州 CCMBM 核心 B：肌肉组织学和临床存储库核心明尼苏达大学肌肉萎缩症诊所照顾最大的肌肉萎缩症群体之一该国的营养不良患者。结合全面的遗传学和组织学诊断由 MDClinic 开发和维护的方法，该人群为 MDCenter 和 NIAMS 肌肉骨骼生物学和医学核心中心。作为其一部分 MDClinic 是综合性临床诊断设施，建立了 DNA、成纤维细胞、来自患有肌肉疾病的受试者的成肌细胞、类淋巴母细胞系和残留的肌肉活检组织；如果特别支持研究目的，该核心将为许多目前资助的人提供巨大的价值 MDCenter 的研究项目，将成为更大的 MD 研究社区的宝贵资产。具体的该核心的目标是： 1) 扩展肌营养不良数据库和 DNA/细胞培养/组织存储库。正式地将此作为研究核心将有助于获取和分发更多样本。 2）使最先进的组织学技术能够充分利用并具有互补性来自患者和模拟人类肌肉疾病的动物的肌肉。这些方法将允许患病肌肉的详细特征以及患者治疗后的比较研究模型系统。 3）提供组织或培养细胞用于详细分析肌肉结构和功能。医学博士中心对临床定义标本的研究对于许多基础和转化项目都具有价值。具体来说，可以直接研究成纤维细胞，将其分化为成肌细胞，以获得全方位的遗传、生化和力生成（核心 C）或用于生成 IPS 细胞（试点 1）以进行发育研究沿着特定遗传疾病的多个细胞谱系