Keratinocyte Core

角质形成细胞核心

基本信息

批准号：
7677671
负责人：
Kathleen Janee Green
金额：
$ 18.93万
依托单位：
NORTHWESTERN UNIVERSITY AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-01 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7677671
关键词：
Adhesions Aerobic Area Biology Boxing Cataloging Catalogs Cell Culture System Cell Culture Techniques Cells Collaborations Collagen Communities Consultations Culture Techniques Defect Dermatology Development Disease Elements Engineering Epidermis Epithelial Epithelial Cells Equipment Evaluation Experimental Designs Factor Analysis Fostering Goals Human Human papillomavirus 16 Hypoxia Investigation Life Maintenance Methods Microscope Modeling Molecular Monitor Mus Pathology Patients Preparation Process Reagent Research Research Personnel Resources Retroviridae Safety Sampling Services Signal Transduction Skin Stratified Epithelium System Techniques Technology Testing Time Training Ultraviolet B Radiation Universities Wound Healing base cell motility cellular imaging cost design efficacy testing genetic manipulation irradiation keratinocyte member novel skills training skin disorder tissue culture tissue/cell culture ultraviolet irradiation

项目摘要

Progress in our understanding of skin biology and disease has been greatly advanced by the use of epidermal cell cultures. The Keratinocyte Core is designed to support and attract new investigators in skin biology by providing a reliable supply of highly purified preparations of human and mouse epidermal keratinocytes at a reduced cost. In addition, the Core will train researchers in the development and use of these various cell culture systems. In cases where more permanent cultures are required, immortalization and long-term culture techniques will be applied to human and mouse keratinocytes. The Core will serve as a resource for: 1) storing these cells and bulk tissue culture supplies; 2) transmitting expertise and techniques for studying keratinocytes; and 3) providing access to larger equipment suitable for keratinocyte biology (e.g., a UV irradiation lamp box, a humidified variable aerobic workstation for hypoxia studies, and a Nikon inverted microscope integrated for live cell imaging of linear scratch wounds). The Core will also provide training and supplies for a three-dimensional organotypic model for human epidermis that permits analysis of factors controlling the formation and organization of this stratified epithelium during development and in wound healing processes. Collectively, the Core will offer the necessary materials and expertise for NU SDRC investigators to better develop novel cell and molecular based strategies towards curing skin diseases and will help foster new collaborations among groups with the common goal of understanding the biology of epithelial cells.

通过使用表皮细胞培养。角质形成细胞核心旨在支持和吸引皮肤中的新研究人员生物学通过提供可靠的人和小鼠表皮的可靠供应角质形成细胞的成本降低。此外，核心将培训研究人员的发展和使用这些各种细胞培养系统。如果需要更多的永久性培养，则永生和长期培养技术将应用于人类和小鼠角质形成细胞。核心将作为一种资源：1）存储这些细胞和大量的组织培养物供应； 2）传输专业知识和研究角质形成细胞的技术； 3）提供适用于角质形成细胞的较大设备生物学（例如，紫外线辐射灯盒，用于缺氧研究的有氧有氧工作站和A 尼康倒置的显微镜集成了线性刮伤伤口的活细胞成像）。核心也将为人类表皮的三维器官模型提供训练和供应分析控制这种分层上皮的形成和组织的因素并在伤口愈合过程中。核心将共同提供必要的材料和专业知识 NU SDRC研究人员更好地开发新的细胞和基于分子的策略来治愈皮肤疾病，将有助于促进群体之间的新合作，其共同目标是理解上皮细胞的生物学。