Core B STEM

核心B STEM

基本信息

批准号：
10455748
负责人：
Kathleen Janee Green
金额：
$ 21.25万
依托单位：
NORTHWESTERN UNIVERSITY AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-20 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10455748
关键词：
3-Dimensional Address Adult Age Architecture Archives Automobile Driving Basic Science Biological Assay Biology Biomedical Engineering Cell Culture Techniques Cell Separation Cell model Cells Cellular Morphology Chemistry Client Collaborations Consultations Culture Media Custom Cutaneous Data Analyses Defect Derivation procedure Dermatology Development Discipline Disease Engineering Epidermis Epithelial Equipment Evaluation Experimental Designs Female Fibroblasts Fresh Tissue Gender Gene Expression Genetic Goals Health Service Area Homeostasis Human Hypersensitivity Imaging technology Immune Immunology Individual Investigation Investigational Drugs Knockout Mice Knowledge Laboratory Research Libraries Longevity Maintenance Methodology Methods Microscope Microscopy Modeling Molecular Morphology Mus Neonatal Neurons Patients Pharmacology Phenotype Physiological Procedures Proteomics Protocols documentation Race Reader Reagent Reporter Research Research Personnel Resources Sampling Services Site Skin Skin Tissue Source Stains Standardization System Technology Therapeutic Tissue Engineering Tissue Sample Tissues Training Transgenic Mice Transglutaminases Translating Translational Research Ultraviolet B Radiation Universities Viral analysis pipeline antibody libraries base biobank cell bank cell immortalization cell type cost demographics drug discovery histological stains human disease human model induced pluripotent stem cell innovation instrument involucrin keratinocyte live cell imaging male mechanical properties melanocyte member mouse model multidisciplinary novel penis foreskin programs promoter protein expression reconstitution repository research and development skin disorder skin morphogenesis three dimensional cell culture three-dimensional modeling tissue culture tool translational study virology

项目摘要

Skin culture models serve as a key tool for understanding normal and diseased skin in well-controlled culture systems that empower investigators to probe skin biology with a rigorous model for mechanistic analysis. 3D skin culture models using primary cells are especially powerful as they closely mimic skin morphogenesis, differentiation, and mechanical properties, leading to a physiologically-relevant tissue architecture. This model is particularly applicable to study mechanisms that govern tissue development, homeostasis and disease. The Skin Tissue Engineering and Morphology (STEM) Core enables SBDRC researchers to apply primary skin culture models in their research program. Towards this aim, the STEM Core provides training, services, specialized equipment, and materials for the initiation, maintenance, processing, and analysis of primary human skin cell cultures, i.e., keratinocytes, melanocytes, fibroblasts, neurons, and immune cells (with the TEST IT Core). The STEM Core also generates short- and long-term mouse keratinocyte cultures for studies aimed at defining the cellular and molecular basis of skin defects evident in engineered mouse models. Users have access to a large supply of primary human keratinocytes isolated from neonatal foreskin, female and male adult skin, and a library of patient keratinocytes. We endeavor to maintain a diverse cell bank with donors of various demographics (age, gender, race, body site, and disease). In collaboration with GET iN Core, the STEM Core optimized methods for viral, pharmacological, and genetic reprogramming of primary skin cells, including induced pluripotent stem cells (iPSCs). Our efforts will also focus on investigator-driven research and development to customize 3D cultures and extend their applicability to address multiple aspects of skin biology from a variety of disciplines including allergy, drug discovery, materials engineering, and chemistry. Training and provision of 3D organotypic models of human epidermis established from control and patient skin cells allows investigators to address skin biology in an architecturally appropriate manner where multiple cell types can interact with one another.

皮肤培养模型是理解良好控制文化中正常皮肤和患病皮肤的关键工具通过严格的模型进行机械分析的系统，可以使研究人员能够探测皮肤生物学。 3D 使用原代细胞的皮肤培养模型特别强大，因为它们紧密模仿皮肤形态发生，分化和机械性能，导致与生理相关的组织结构。这个模型特别适用于研究组织发育，稳态和疾病的机制。这皮肤组织工程和形态（STEM）核心使SBDRC研究人员能够应用原发性皮肤他们的研究计划中的文化模型。为了实现这一目标，STEM核心提供培训，服务，专门的设备和材料用于初级人类的启动，维护，处理和分析皮肤细胞培养物，即角质形成细胞，黑素细胞，成纤维细胞，神经元和免疫细胞（在测试中核）。茎芯还产生了短期和长期的小鼠角质形成细胞培养物，用于针对的研究在工程小鼠模型中定义皮肤缺陷的细胞和分子基础。用户可以访问从新生儿包皮，雌性和雄性皮肤中分离出的大量原代人角质形成细胞，以及患者角质形成细胞的库。我们努力与各种捐助者保持多样化的细胞库人口统计学（年龄，性别，种族，身体部位和疾病）。与Get In Core合作，STEM核心对原代皮细胞的病毒，药理和遗传重编程的优化方法，包括诱导多能干细胞（IPSC）。我们的努力还将集中于研究人员驱动的研究和发展自定义3D文化并扩展其适用性，以解决各种皮肤生物学的多个方面包括过敏，药物发现，材料工程和化学的学科。培训和提供3D 由对照和患者皮肤细胞建立的人类表皮的器官模型使研究人员能够以一种适当的方式解决皮肤生物学，其中多种细胞类型可以与一种相互作用其他。