Transcriptional Regulation of Cervical Competence During Pregnanc

怀孕期间宫颈功能的转录调控

• 批准号: 7721063
• 负责人: RUTH A WORD
• 金额: $ 23.53万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721063
• 关键词: Cell Line; Cells; Cervical; Cervix Uteri; Clinical; Collection; Competence; Consent Forms; Core Facility; Cultured Cells; Databases; Decidua; Discipline of obstetrics; Endometrium; Equipment and Supplies; Fetal Tissues; Fixatives; Freezing; Funding; Gestational Age; Gynecology; Laboratories; Laboratory culture; Location; Maintenance; Medical; Obstetrics Departments; Pathology; Placenta; Plasma; Procedures; Process; Protocols documentation; Records; Research Ethics Committees; Research Personnel; Sampling; Services; Standardization; Support of Research; Teaching Hospitals; Tissue Sample; Tissues; Transcriptional Regulation; Wages; Week; Work; abortion; amnion; base; cell type; cost; experience; human tissue; myometrium; programs; repository; research study; tissue/cell culture; tissue/cell preparation

Core A: Human Tissue Acquisition and Cell Culture Laboratory INTRODUCTION. The availability of human tissues is a necessity for the conduct of experiments within each of the projects, and the investigators collectively have many years of experience with experimentation involving human tissue samples. The Program Project has for more than 20 years maintained an excellent working relationship with the Department of Pathology at our teaching hospital and with the staffs of the obstetric and gynecological services in the Department of Obstetrics and Gynecology. The current arrangement was built on that historical base and modified as needed to accommodate specific needs of the projects and to keep abreast of compliance issues related to use and procurement of human tissues. Working with components of the Administrative Core, this centralized process for collection, processing, and culturing of human tissues and cells has proven to be e'<peditious and cost-effective. In addition, it provides a consistency in acquisition and processing and a standardization of tissue preparation and cell culturing that is extremely beneficial to the investigative process. A repository of frozen tissue samples is also maintained within this Core laboratory. The stored samples include myometrium, endometrium, cervix, placenta, amnion, decidua, and cord blood plasma collected over the last several years; also stored are samples of selected fetal tissues (12-20 weeks gestational age) collected during a previous program under an approved protocol for obtaining tissues from elective abortions. A database is maintained of the stored tissues, their locations, and relevant collection and medical information. In addition to obtaining tissues for use by Program Project investigators, the Department of Obstetrics and Gynecology is now able to fund partial salary support for research personnel so that this tissue acquisition program is available to other investigators in the Department of Obstetrics and Gynecology as well as other departments on campus, provided they have IRB-approved protocols.

核心A：人体组织获取和细胞培养实验室 介绍。人体组织的可用性是进行每个组织进行实验的必要性 在项目中，调查人员在涉及的实验方面有多年的经验 人体组织样品。该计划项目已有20多年了 与我们教学医院的病理部以及产科工作人员的关系 妇产科和妇科系的妇科服务。当前的安排建立在 那个历史基础和根据需要修改，以适应项目的特定需求并保留 与人类组织的使用和采购有关的合规性问题并肩。与组件一起工作 行政核心，这种集中的人类组织的收集，加工和培养过程 细胞已被证明是<良好且具有成本效益的。此外，它在获取和 对组织制备和细胞培养的加工和标准化对 调查过程。 在该核心实验室内还保留了冷冻组织样品的存储库。存储 样品包括子宫内膜，子宫颈，胎盘，羊膜，Decidua和脐带血等离子体 在过去的几年中收集；还存储的是选定的胎儿组织的样品（12-20周 胎龄）在以前的程序中收集的审核方案中收集的胎龄，用于从 选修课。维护存储组织，其位置和相关收集的数据库以及 医疗信息。 除了获得计划调查人员使用的组织外，妇产科 妇科现在能够为研究人员提供部分工资支持，因此该组织获取 妇产科和其他研究人员以及其他调查员都可以使用计划 只要他们拥有IRB批准的协议，校园的部门。