BIOCHEMICAL AND MOLECULAR BIOLOGY CORE LABORATORY

生化与分子生物学核心实验室

• 批准号: 7600722
• 负责人: David Rae Harder
• 金额: $ 30.75万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7600722
• 关键词: Animal Model; Arts; Biochemical; Biological Assay; Cardiovascular system; Chemicals; Consultations; Core Facility; Equipment; Functional disorder; Gene Expression; Guidelines; Hand; Human Resources; Laboratories; Measures; Messenger RNA; Metabolic; Modeling; Molecular; Molecular Biology; Molecular and Cellular Biology; Myocardial Infarction; Polymerase Chain Reaction; Procedures; Program Research Project Grants; Quality Control; Records; Research Personnel; Running; Sampling; Services; Small Interfering RNA; Techniques; Technology; Time; Training; computerized; experience; programs; training project

This Biochemical and Molecular Core facility, under the direction of Dr. David Harder with the assistance of Jayashree Narayanan, will provide a centralized professional service to all three projects involved in this Program. The staff will be trained by the project leaders or specialized personnel to perform a variety of procedures required in this Program Project Grant. This facility will maintain complete, computerized and centralized records and well-defined quality control guidelines for all assays. In addition new assays will be developed by Dr. Harder in consultation with investigators to meet the changing needs and directions of the Program. New assays that have thus far been developed by the Core include siRNA technology to silence gene expression, real time PCR to quantitate mRNA levels, metabolic models to record endothelial dysfunction and animal models to measure myocardial infarction. Dr. Harder and Ms. Narayanan have first hand experience in running core laboratories for two other Program Projects. The Core facility is located in the Cardiovascular Center which is in close proximity to the laboratories of the investigators for all three projects.

这种生化和分子核心设施，在戴维·哈德尔博士的指导下 Jayashree Narayanan的协助将为所有三个项目提供集中的专业服务 参与此程序。工作人员将受项目负责人或专业人员培训，以执行 此计划项目赠款中需要的各种程序。该设施将保持完整， 所有测定法的计算机和集中记录以及定义明确的质量控制指南。此外 Harder博士将与调查人员协商以满足不断变化的需求，将开发新的测定 和程序的指示。 迄今为止，核心开发的新测定包括siRNA技术以保持沉默 基因表达，实时PCR来定量mRNA水平，代谢模型以记录内皮 功能障碍和动物模型测量心肌梗塞。 Harder博士和Narayanan女士首先 在其他两个计划项目中运行核心实验室的手工经验。核心设施位于 这三个的心血管中心与调查人员的实验室非常接近 项目。