Human Tissue Procurement and Processing Core

人体组织采购和加工核心

基本信息

批准号：
10707550
负责人：
Stephanie Shiers
金额：
$ 51.88万
依托单位：
UNIVERSITY OF TEXAS DALLAS
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-19 至 2027-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10707550
关键词：
Autopsy Award Biological Assay Chest Chronic disabling pain Collaborations Country Data Set Data Sources Development Dissociation Funding Goals Hospitals Human Institutional Review Boards Intervention Studies Knowledge Laboratories Low Back Pain Lumbar nerve Maps Methods Molecular Molecular Profiling Nervous System Neurons Nociceptors Nodose Ganglion Operative Surgical Procedures Organ Donations Organ Donor Organ Transplantation Pain Pain Research Patients Peripheral Nerves Persistent pain Phenotype Physicians Price Procedures Proliferating Protocols documentation Publications Quality Control RNA Recording of previous events Recovery Research Research Design Resected Resources Sampling Site Slice Spinal Cord Spinal Ganglia Technology Therapeutic Tissue Procurements Tissues Transplantation United States United States National Institutes of Health University of Texas M D Anderson Cancer Center Work arthropathies chronic pain experience human tissue pain patient timeline tissue processing tissue resource transcriptome transcriptomics

项目摘要

Work with human dorsal root ganglia (DRG) and spinal cords has been transformed by collaborative efforts with organ transplant recovery agencies. Over the past years, we have developed an expansive collaboration with organ transplant agencies enabling recovery of more than 60 sets of DRGs and spinal cords from organ donors. This collaboration has led to several co-authored publications and the development of new methods to recover additional nervous system tissues from surgical patients at hospitals involved in this project. A key question in the pain field is whether human nociceptors change their molecular makeup when a patient develops persistent or chronic pain. We will use the unique resources of this core to directly answer this question, and generate datasets to answer new questions for the pain therapeutic discovery field. Our first aim will build on our existing work with organ donors to create administrative and scientific protocols for tissue recovery. A key milestone will be the recovery of DRG, spinal cord and peripheral nerves from 50 organ donors per year. In our second aim we will expand our unique access to DRGs from pain patients that are recovered during relatively rare surgeries. To facilitate more of this type of research throughout the country, and enhance access to these valuable tissues, we will create IRB templates, hospital compliant standard operating procedures (SOPs) and other key administrative and scientific methods to enable the proliferation of this type of research. Finally, in our third aim we will leverage our deep experience with RNAscope technology to compare expression of a group of markers that vary in cellular expression (low, mid, and high expression) to understand exactly how tissue transcriptomes might change depending on donor timeline and other factors. This will allow the field to understand how to make comparisons across study types. This core will create an expansive resource of tissue, technical methods and administrative proficiency that will enable important discoveries for the field, and the expansion of the use of human nervous system tissues for basic pain research far beyond the walls of the collaborating laboratories in this Center.

人类背根神经节 (DRG) 和脊髓的工作已经通过协作而改变与器官移植恢复机构的努力。在过去的几年里，我们开发了广泛的与器官移植机构合作，实现 60 多套 DRG 和脊髓的恢复来自器官捐献者。这种合作导致了一些共同撰写的出版物和新的开发从参与该项目的医院的手术患者身上回收额外神经系统组织的方法。疼痛领域的一个关键问题是，当患者疼痛时，人类伤害感受器是否会改变其分子组成出现持续性或慢性疼痛。我们将利用这个核心的独特资源来直接回答这个问题，并生成数据集来回答疼痛治疗发现领域的新问题。我们的首要目标是建立关于我们与器官捐赠者现有的合作，以制定组织恢复的行政和科学协议。一把钥匙里程碑将是每年从 50 名器官捐献者身上恢复 DRG、脊髓和周围神经。在我们的第二个目标是，我们将扩大我们从疼痛患者那里获得 DRG 的独特途径，这些患者在相对罕见的手术。为了促进全国范围内更多此类研究的开展，并增加获得这些研究的机会有价值的组织，我们将创建 IRB 模板、符合医院要求的标准操作程序 (SOP) 和其他关键的管理和科学方法，以促进此类研究的扩散。最后，在我们的第三个目标，我们将利用我们在 RNAscope 技术方面的丰富经验来比较一组细胞表达不同（低、中和高表达）的标记物可准确了解组织如何转录组可能会根据捐赠者的时间线和其他因素而改变。这将使该领域了解如何对不同研究类型进行比较。这个核心将创造出广泛的组织资源，能够为该领域带来重要发现的技术方法和管理能力，以及将人类神经系统组织用于基础疼痛研究的用途扩展到远远超出了实验室的范围该中心的合作实验室。