Assessing the interplay between stress, health, behavior, and inflammatory gene expression response to wildfire smoke exposures using community engaged and remote sampling approaches - Brown Div Supp

使用社区参与和远程采样方法评估压力、健康、行为和炎症基因表达对野火烟雾暴露的反应之间的相互作用 - Brown Div Supp

• 批准号: 10707654
• 负责人: Nicole Ann Errett
• 金额: $ 3.23万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-28 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10707654
• 关键词: Affect; American; Area; Behavior; Biological; Blood; Blood specimen; Collaborations; Collection; Communication; Communities; Data; Development; Enrollment; Event; Evidence based intervention; Frequencies; Gene Expression; Genes; Health; Household; Human; Immune system; Inflammatory; Inflammatory Response; Intervention; Knowledge; Occupational; Pacific Northwest; Participant; Perception; Personal Satisfaction; RNA; Recommendation; Research; Sampling; Science; Seasons; Smoke; Social Impacts; Stress; Surveys; Time; Translational Research; Whole Blood; Wildfire; air cleaner; behavior change; community engagement; evidence base; experience; exposure reduction interventions; improved; member; nano-string; novel; portability; protective effect; response; socioeconomics; transcriptome

PROJECT SUMMARY/ABSTRACT As wildfire occurrence and intensity has increased in recent years, understanding the human impact of wildfire smoke (WFS) exposure has become paramount. The impact of WFS on a community can be examined by investigating the proximal deleterious biological effects of WFS exposure, health and well-being and perceived impact of evidence-based exposure reduction interventions at community scale. At the beginning of the 2021 WFS season, we commenced preliminary research to gauge the biological and social impact of WFS in the Methow Valley, WA; a region in the Pacific Northwest with historically high WFS exposure burden, where by early July 2021, had experienced several weeks of WFS exposure that was “nearly off the chart hazardous”[2]. We distributed a novel kit, homeRNA, for self-collection and stabilization of whole blood RNA that allows for the assessment of inflammatory gene response to WFS to 62 participants across the American West, including 18 in the Methow Valley, WA, collecting over 400 stabilized blood samples to date: a subset of which will be analyzed with a NanoString panel of ~800 inflammatory genes. Concomitantly, leveraging the donation and distribution of 2,000 HEPA portable air cleaners (PACs) to community members across Methow Valley, WA, we enrolled and surveyed nearly 1,000 participants to assess their perceived health and well-being during WFS events, and the perceived protective effect of the HEPA PACs. This unprecedented opportunity allows us to simultaneously probe into the inflammatory gene response effects of WFS exposure, and perceptions regarding the health and well-being impacts of WFS exposure and mitigation strategies. Here we seek to assess the biological effect of WFS in the inflammatory gene response by identifying key inflammatory response genes in blood before, during, and after exposure using our homeRNA blood sampling kits. We also aim to assess perceived WFS impacts on health, well-being, and behavior change across socio-economic, demographic, occupational, and household groups, as well as the perceived impacts of HEPA PACs, an evidence-based exposure reduction intervention increasingly used and recommended in WFS-affected communities. Together, these results will inform the development of a subsequent R01 application that will combine homeRNA and survey research to improve mechanistic understanding of the effects of WFS exposure and evidence-based exposure reduction interventions (like HEPA PACs) on inflammatory gene expression, behavior and perceived health and well-being. Our long-standing, genuine partnership with the Methow Valley community honed through years of collaboration enables this research, and uniquely allows for real-time science communication and research translation. As wildfires are expected to increase in frequency and magnitude, enhancing our scientific knowledge about health impacts and their underlying mechanisms, as well the impact of interventions, is of immediate and paramount importance.

项目摘要/摘要 随着野火发生和强度近年来的增加，了解人类的影响 野火烟雾（WFS）暴露已成为最重要的。可以检查WFS对社区的影响 通过研究WFS暴露，健康和福祉和感知的近端有害生物学作用 在社区规模上减少基于证据的暴露干预措施的影响。在2021年初 WFS季节，我们开始了初步研究，以评估WFS在 华盛顿州方法谷；西北太平洋地区有历史悠久的WFS曝光伯恩的一个地区， 2021年7月上旬，经历了几周的WFS暴露，“几乎是危险的危险” [2]。 我们分发了一个新颖的套件Homerna，以进行全血RNA的自我收集和稳定，这允许 评估炎症基因对WFS对整个美国西部62名参与者的反应，其中包括18个 迄今 用〜800个炎症基因的纳米串面板分析。同时利用捐赠和 向华盛顿州Method Valley的社区成员分配2,000 hepa便携式空气清洁剂（PAC），我们 参加并调查了近1,000名参与者，以评估其在WFS期间的健康和福祉 事件以及HEPA PAC的受保护作用。这个前所未有的机会使我们能够 类似地探究了WFS暴露的炎症基因反应影响，以及对 WFS暴露和缓解策略的健康和福祉影响。在这里，我们试图评估 WFS在炎症基因反应中的生物学作用，通过鉴定关键炎症反应基因 使用我们的HOMERNA血液采样套件，在暴露之前和之后的血液。我们还旨在评估 感知的WF会对社会经济，人口，人口，福祉和行为改变的影响 职业和家庭团体以及HEPA PAC的感知影响，这是一个基于循证的 在受WFS影响的社区中，越来越多地使用并建议减少暴露干预措施。一起， 这些结果将告知随后的R01应用程序的开发，该应用程序将结合Homerna和 调查研究以提高对WFS暴露和基于证据的影响的机械理解 在炎症基因表达，行为和感知到的有关炎症基因表达的暴露干预措施（例如HEPA PAC） 健康与福祉。我们与Method Valley社区的长期，真正的合作伙伴关系 多年的合作能够实现这项研究，并唯一允许实时科学沟通和 研究翻译。由于预计野火的频率和幅度将增加，从而增强了我们的科学 关于健康影响及其潜在机制的知识以及干预的影响， 直接和至关重要。