Mali International Center for Excellence in Research: Filariasis

马里国际卓越研究中心：丝虫病

• 批准号: 10692119
• 负责人: Thomas Nutman
• 金额: $ 21.63万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10692119
• 关键词: Address; Aftercare; Antigens; Area; CCL22 gene; Clinical; Communicable Diseases; Country; Data; Dentistry; Developing Countries; Dipetalonema Infections; Doxycycline; Emerging Communicable Diseases; Endemic Diseases; Enrollment; Entomology; Faculty; Filaria bancrofti; Filarial Elephantiases; Filariasis; Fostering; Future; Goals; HIV; IgG4; Immunologics; India; Infection; Leishmania major; Lymphedema; Malaria; Mali; Medicine; Methods; Onchocerciasis; Parasites; Patients; Performance; Persons; Pharmacy facility; Phlebotominae; Physicians; Prevalence; Research; Salivary Proteins; Scientist; Senegal; Serology; Site; Soaps; Testing; Training; Tuberculosis; Uganda; Universities; Up-Regulation; Water; base; burden of illness; co-infection; cohort; efficacy evaluation; follow-up; international center; laboratory facility; monocyte; programs; rapid test; recruit; response; tool; vector

Over the past year, our research in Mali has made progress as follows: 1) We assessed the performance of the SD Bioline Onchocerciasis/LF IgG4 Rapid Test as an integrated surveillance tool for lymphatic filariasis (LF) and onchocericiasis elimination. Our study indicated that this test can be used in post-treatment surveillance and potentially will supplant other approaches that are currently available. 2) Using integrated surveillance tools to evaluate the potential of elimination of both onchocerciasis and lymphatic filariasis, we performed serologic assessment of >6000 people in a formerly highly endemic region of Mali that sits at the border with Senegal. Our data suggests that this elimination is likely. 3) Having determined the prevalence of co-infection with filarial (Wuchereria bancrofti and Mansonella perstans) and Leishmania major parasites in two ecologically distinct areas of Mali we performed immunologic analyses to examine the effect of filarial infection on the innate response to sandfly-derived salivary proteins Our data suggests that filarial antigens drive upregulation of CCL22 that in turn drives monocyte recruitment, but that they fail to alter the response to salivary proteins from the sandfly vector. 5) We have completed the 24month followup in a cohort of 200 patients enrolled in a multi-national, multi-site study to examine the efficacy of a 6 week course of doxycycline on lymphedema in W. bancrofti infection. Data indicate that doxycycline adds very little to the reversal of lymphedema, though hygienic methods (soap/treatment of intertriginous infections/clean water) provides significant benefit.

在过去的一年中，我们在马里的研究取得了如下的进步： 1）我们评估了SD Bioline on Chocerciasis/LF IgG4快速测试的性能，作为用于淋巴丝虫病（LF）和消除术的综合监测工具。我们的研究表明，该测试可用于治疗后监视，并有可能取代当前可用的其他方法。 2）使用综合监视工具来评估消除尾尾c和淋巴丝虫病的潜力，我们在与塞内加尔接壤的Mali地区高度流行地区进行了> 6000人的血清学评估。 我们的数据表明，这种消除可能是可能的。 3）在确定了与丝状（Wucheria bancrofti和Mansonella perstans）和利什曼原虫（Leishmania perstans）和利什曼原虫（Leishmania）的主要寄生虫中，在两个生态上不同的马里地区，我们进行了免疫学分析，我们进行了免疫学分析，以检查丝面感染对Sandfly-derfipary prodec的天生反应的影响。单核细胞募集，但他们无法改变Sandfly载体对唾液蛋白的反应。 5）我们已经完成了一项跨国多站点研究的200名患者的队列中的24个月随访，以研究W. bancrofti感染中多克环素6周淋巴水肿的疗效。数据表明，强力霉素几乎增加了淋巴水肿的逆转，尽管卫生方法（肥皂/跨层感染/清水的肥皂/治疗）提供了很大的好处。