Dynamic regulation of neuroinflammation and IL-1b-trap

神经炎症和 IL-1b-trap 的动态调节

基本信息

批准号：
10726269
负责人：
Ning Quan
金额：
$ 41.94万
依托单位：
FLORIDA ATLANTIC UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10726269
关键词：
Anabolism Animals Anxiety Autoimmune Behavior Behavioral Brain Breeding Cells Cellular biology Central Nervous System Diseases Clinical Research DNA Sequence Epilepsy Erythrocytes Fever Genetic Genetic Recombination Hippocampus Homeostasis IL1B gene IL1R1 gene Immune Immunohistochemistry In Situ Hybridization Inflammatory Interleukin Activation Interleukin-1 Interleukins Internal Ribosome Entry Site Kainic Acid Knock-in Learning Maps Mediating Memory Mental disorders Messenger RNA Methods Modeling Moods Mus Nerve Degeneration Neuroimmune Neurons Oranges Pathogenesis Pathogenicity Peripheral Pharmacotherapy Physiological Population Regulation Reporter Research Role Severities Sleep Slow-Wave Sleep Source Terminator Codon Visualization behavioral outcome cytokine depressive symptoms design designer receptors exclusively activated by designer drugs excitotoxicity genetic manipulation insight invention nervous system disorder neural circuit neurochemistry neuroinflammation preclinical study prevent receptor tool

项目摘要

PROJECT SUMMARY IL-1 is a master neuroimmune regulator. IL-1 activity is important for both physiological functions of the brain and pathogenesis of all CNS diseases. The cellular sources of IL-1 are not well defined and genetic access to manipulating IL-1-expressing cells is lacking. We have created an IL-1-trap mouse to visualize IL-1 expressing cells. In addition, we can inhibit or activate IL- 1-expressing cells by chemogenetic methods. Using this new tool, we will: 1) demonstrate distinct sets of cells express IL-1 in homeostasis and in different neuroimmune activation conditions; 2) determine the role of homeostatic IL-1-expressing cells on the neurochemical and behavioral consequences following peripheral and central inflammatory challenge; and 3) determine the role of the induced IL-1-expressing cells on the neurochemical and behavioral consequences following peripheral and central inflammatory challenges.

项目概要 IL-1 是主要的神经免疫调节剂 IL-1 活性对于这两种生理功能都很重要。 IL-1 的细胞来源尚不清楚。我们已经创建了操作 IL-1 表达细胞的定义和遗传途径。 IL-1-trap 小鼠可观察 IL-1 表达细胞。此外，我们还可以抑制或激活 IL-1 表达细胞。通过化学遗传学方法表达 1 的细胞，我们将：1) 证明。不同的细胞组在稳态和不同的神经免疫激活中表达 IL-1 条件；2) 确定表达 IL-1 的稳态细胞对神经化学和外周和中枢炎症挑战后的行为后果；3) 确定诱导的 IL-1 表达细胞对神经化学和行为的作用外周和中枢炎症挑战后的后果。