A novel mammalian transcription factor involved in HSV

一种参与 HSV 的新型哺乳动物转录因子

• 批准号: 6503201
• 负责人: THOMAS M KRISTIE
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6503201
• 关键词: Alphaherpesvirinae; gene expression; genetic enhancer element; genetic regulation; nucleic acid sequence; polymerase chain reaction; protein protein interaction; protein structure function; regulatory gene; transcription factor; virus genetics; virus protein; yeast two hybrid system

The regulation of herpes simplex virus immediate early gene (IE) expression is governed by viral and cellular proteins that assemble a multiprotein transcription enhancer complex. The analysis of the protein components and the biochemical interactions provides a model for RNAPII directed transcription and identifies critical elements of the HSV IE gene regulatory mechanism. One such component, the cellular C1 factor, is a large and complex family of polypeptides which functions to direct the assembly of the enhancer complex as well as to mediate the transcriptional activation potential of some of the associated polypeptides. An unusual characteristic of the C1 factor is the autocatalytic processing that generates the C1 family of polypeptides. Isolation of a number of cellular proteins that specifically bind the reiterated processing sites indicates that processing may serve to regulate protein-protein interactions and thus the functions of the C1 factor.

立即的早期基因（IE）表达的疱疹病毒的调节受病毒和细胞蛋白的控制，这些病毒和细胞蛋白组装了多蛋白转录增强剂复合物。蛋白质成分和生化相互作用的分析为RNAPII定向转录提供了模型，并确定了HSV IE基因调节机制的关键元素。一种这样的成分，即细胞C1因子，是一个大而复杂的多肽家族，其功能可以指导增强剂复合物的组装以及介导某些相关多肽的转录激活潜力。 C1因子的不寻常特征是产生C1多肽家族的自催化处理。分离许多特异性结合重复处理位点的细胞蛋白的分离表明，加工可以调节蛋白质 - 蛋白质相互作用，从而调节C1因子的功能。