Defining epigenetic dysregulation in aged hematopoietic stem cells

定义衰老造血干细胞的表观遗传失调

• 批准号: 10688897
• 负责人: Isabel Beerman
• 金额: $ 46.75万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10688897
• 关键词: ATAC-seq; Aberrant DNA Methylation; Address; Age; Aging; Automobile Driving; Biology; Caloric Restriction; Cell Compartmentation; Cell physiology; Cells; ChIP-seq; Chromatin Structure; Code; Collaborations; DNA Methylation; Epigenetic Process; Flow Cytometry; Frequencies; Gene Expression; Gene Expression Profile; Genetic Transcription; Genomics; Hematopoietic stem cells; Histones; Intervention; Laboratories; Lead; Malignant Neoplasms; Mus; Play; Publishing; RNA; RNA analysis; Resources; Role; Testing; Transcriptional Regulation; Untranslated RNA; adult stem cell; age related; aged; aging population; cell community; circular RNA; functional loss; hematopoietic stem cell aging; human old age (65+); insight; large datasets; mRNA sequencing; male; prevent; regeneration potential; senescence; stem cells

We have generated transcriptional analysis (mRNA-seq) of HSCs isolated from replicates of young and old age populations. We have additionally evaluated 5 histone marks, ATAC-seq, and ChIP-seq from HSCs purified from both young and aged male C57Bl/6J mice. Age-related changes showed showed significant changes in bivalent domains, contact frequency and overall chromatin structure associated with to aberrant transcriptional profiles. We are currently working to publish these large dataset generated as a resource to the aging and stem cell community.

我们已经从年轻人和老年人的重复分离的HSC产生了转录分析（mRNA-SEQ）。 我们还评估了从年轻和老年男性C57BL/6J小鼠纯化的HSC的5个组蛋白标记，ATAC-SEQ和CHIP-SEQ。 与年龄相关的变化显示，与异常转录曲线相关的二价结构域，接触频率和总体染色质结构的显着变化。 我们目前正在努力将这些大型数据集发布为衰老和干细胞社区的资源。