Cell Biology

细胞生物学

• 批准号: 10669728
• 负责人: Jun Yang
• 金额: $ 15.09万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10669728
• 关键词: Area; Award; Basic Science; Cell Culture Techniques; Cells; Cellular biology; Collaborations; Consultations; Cryopreservation; Cryoultramicrotomy; Disease; Evolution; Faculty; Future; Generations; Genotype; Glaucoma; Individual; Laboratories; Mus; Organoids; Paraffin Embedding; Research; Research Personnel; Research Project Grants; Resources; Retina; Retinal Degeneration; Services; System; Technical Expertise; Training; Translational Research; Universities; Utah; Vision research; developmental disease; eye center; immunocytochemistry; induced pluripotent stem cell; mouse genetics; preservation; tissue culture; working group

Project Summary-Cell Biology Core The OVERALL aims of this Vision Research Core (VRC) are to provide: • access to resources outside the scope of individual R01 awards • access to technical expertise outside the scope a single laboratory • staff training to remove barriers to efficient translational research and collaboration • collaboration initiatives among VRC labs The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 17 investigators holding 22 NEI R01 awards. The Cell Biology Module derives from the strengths in mouse genetics at the Moran Eye Center and the high demand for tissue culture and profiling. Almost every JMEC laboratory has need of high quality resources for cryosectioning, paratoming, genotyping, immunocytochemistry, quantitative PCR (qPCR), cell culturing, and mouse colony preservation. Some JMEC laboratories will have future need for induced pluripotent stem cell (iPSC) generation and retinal organoid differentiation. Specifically the Cell Biology module provides: • High-quality cryotomy resources through two professionally maintained Leica CM3050S systems • Paraffin embedding and section services via a Leica Paratome • PCR and genotyping services • Training of cryosectioning, immunocytochemistry, genotyping, and qPCR • Cell culture resources through access to a well-equipped and maintained biosafety level 2 (BSL2) cell culture facility • Technical consultation for mouse line cryopreservation, iPSC generation and retinal organoid differentiation

项目摘要细胞生物学核心 该愿景研究核心（VRC）的总体目标是提供： •访问个人R01奖项范围之外的资源 •范围外的技术专长访问一个实验室 •员工培训以消除有效的转化研究和协作的障碍 •VRC实验室的合作计划 VRC支持的研究领域跨越了残余变性的分析和处理， 发育障碍，青光眼和其他疾病以及一系列尖端的基础科学 倡议。我们已经实施了四个资源模块，这些模块继续了这种方式的自然演变 研究小组共同合作，为17名持有22个NEI R01奖项的调查人员提供服务。 细胞生物学模块来自Moran眼中的小鼠遗传学的强度和高 对组织培养和分析的需求。几乎每个JMEC实验室都需要高质量的资源来实现 冷冻，寄生虫，基因分型，免疫细胞化学，定量PCR（QPCR），细胞培养和 小鼠菌落保存。一些JMEC实验室将有未来的诱导多能干细胞的需求 （IPSC）产生和视网膜器官分化。 特别是细胞生物学模块提供： •通过两个专业维护的Leica CM3050S系统，高质量的冷冻切开术资源 •通过Leica Paratome的石蜡嵌入和部分服务 •PCR和基因分型服务 •培训冷冻培训，免疫细胞化学，基因分型和QPCR •通过访问设备齐全和维护的生物安全2（BSL2）细胞，细胞培养资源 文化设施 •鼠标线冷冻保存，IPSC生成和视网膜器官的技术咨询 分化