Histopathology and Microscopy Core

组织病理学和显微镜核心

基本信息

批准号：
10711695
负责人：
VOLKHARD LINDNER
金额：
$ 31.09万
依托单位：
MAINEHEALTH
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-01 至 2028-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10711695
关键词：
3-Dimensional Adipocytes Adipose tissue Antibodies Antigens Automation Biochemical Biochemical Pathway Biological Biology Blood Vessels Bone Tissue Cell Culture Techniques Cells Cellular biology Centers of Research Excellence Charge Client Clinical Communities Confocal Microscopy Consultations Core Facility Custom Dedications Equipment Fluorescence Resonance Energy Transfer Freezing Funding Funding Mechanisms Goals Grant Hazardous Waste Histology Histopathology Histopathology Facility Image Immunohistochemistry Investments Laboratories Maine Manuals Medical center Mesenchymal Microscope Microscopy Monoclonal Antibodies National Institute of General Medical Sciences New England Outcome Paraffin Embedding Pathology Phase Physicians Physiology Plastic Embedding Postdoctoral Fellow Price Procedures Production Protein translocation Proteins Publications Research Research Personnel Resource Sharing Resources Retrieval Running SP1 gene Scientist Services Sister Solvents Specimen Handling Technical Expertise Techniques Tissue Embedding Tissue Microarray Tissue Stains Tissues Training United States National Institutes of Health Universities Validation Workload cost detection method experience histological stains improved instrumentation medical specialties member mineralization morphometry neuroregulation operation polyclonal antibody sample fixation skeletal stem stem cells success tissue processing

项目摘要

Histopathology and Microscopy Core (V. Lindner, Core Director) The current Histopathology and Microscopy Core was established in 2004 within the COBRE in Stem and Progenitor Cell Biology (8P20GM103465), with Dr. Volkhard Lindner as the Core Director. Services included fixation, embedding, and processing of specimens for frozen and paraffin embedded sections and a spectrum of routine and specialty histological stains and basic immunohistochemistry. Our staff has expertise to develop and optimize immunohistochemistry procedures, including antigen retrieval, optimization in archival tissues, and various detection methods. The success of this Core attracted users from our sister COBRE in Vascular Biology, other investigators at Maine Medical Center, and regional clients, including from neighboring IDeA states. Our services were in high demand, and led in 2011 to our COBRE in Vascular Biology (P30GM103392) co-funding the Histopathology Core to increase research staff for expanded capacity, and add tissue microarray services. This co-funding mechanism has been extremely effective, allowing for efficient expansion of services. The Core with its current staff (Core Director and two full-time staff members) performs more than 30,000 procedures annually and in the past 14 years has directly supported close to 200 publications. During Phase I of this COBRE in Mesenchymal and Neural Regulation of Metabolic Networks, plastic embedding and sectioning as well as histomorphometry were established to meet demands by COBRE projects. To improve efficiency, mineralized tissue histology services have now been consolidated with services provided by the COBRE supported Histology and Imaging Core at the nearby University of New England. Efficiency and capacities were also increased during Phase I by acquisition of a larger tissue processor that uses solvents much more efficiently, thereby reducing costs and hazardous waste. As part of Phase II of this COBRE application, we propose to integrate microscopy and confocal microscopy services under the directorship of Dr. Prudovsky to meet the needs of Phase II supported projects and other federally funded research. Aim 1 of this core is to continue to provide outstanding services and training in a full suite of histopathology services. Aim 2 is to seamlessly integrate microscopy and confocal microscopy services and training into our workflow, to better serve the needs of our COBRE investigators and other users of this shared resource. COBRE investigators will receive these services at no charge, and other investigators enjoy highly competitive pricing. As in the past, the Core will also continue to offer its extensive and growing spectrum of services related to histology with full utilization of existing equipment and instrumentation garnered from previous COBRE support, as encouraged by NIGMS.

组织病理学和显微镜检查核心（V. Lindner，核心主任）目前的组织病理学和显微镜检查核心于 2004 年在 COBRE 内建立，位于干细胞和祖细胞生物学 (8P20GM103465)，由 Volkhard Lindner 博士担任核心主任。包含的服务冷冻和石蜡包埋切片和光谱标本的固定、包埋和处理常规和特殊组织学染色以及基础免疫组织化学。我们的员工拥有可发展的专业知识并优化免疫组织化学程序，包括抗原修复、档案组织的优化，以及各种检测方法。该 Core 的成功吸引了我们血管领域姊妹公司 COBRE 的用户生物学、缅因州医疗中心的其他研究人员以及区域客户，包括来自邻近 IDeA 的客户州。我们的服务需求量很大，并于 2011 年获得了血管生物学 COBRE (P30GM103392) 共同资助组织病理学核心，以增加研究人员以扩大能力，并增加组织微阵列服务。这种共同资助机制非常有效，可以实现高效扩张的服务。核心及其现有员工（核心总监和两名全职员工）的表现超过每年 30,000 个程序，在过去 14 年中直接支持了近 200 种出版物。期间 COBRE 的第一阶段在代谢网络的间充质和神经调节、塑料嵌入和切片和组织形态计量学的建立是为了满足 COBRE 项目的需求。改善效率，矿化组织组织学服务现已与以下机构提供的服务合并： COBRE 支持附近新英格兰大学的组织学和成像核心。效率和通过收购使用溶剂的更大的组织加工机，第一阶段的产能也得到了提高更加有效，从而降低成本和危险废物。作为 COBRE 第二阶段的一部分应用程序，我们建议在博士的领导下整合显微镜和共焦显微镜服务。 Prudovsky 以满足第二阶段支持项目和其他联邦资助研究的需求。这个目标1 核心是继续提供出色的服务和全套组织病理学服务的培训。目标2 是将显微镜和共焦显微镜服务和培训无缝集成到我们的工作流程中，更好地满足我们的 COBRE 调查人员和该共享资源的其他用户的需求。科布雷调查员将免费获得这些服务，而其他调查员则享受极具竞争力的价格。与过去一样，核心还将继续提供与以下领域相关的广泛且不断增长的服务范围：充分利用以前 COBRE 获得的现有设备和仪器进行组织学研究 NIGMS 鼓励的支持。