Core C TEST IT

核心 C 测试

基本信息

批准号：
10259798
负责人：
I. Caroline Le Poole
金额：
$ 18.54万
依托单位：
NORTHWESTERN UNIVERSITY AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-20 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10259798
关键词：
3-Dimensional Affect Antibodies Antigens Area Biological Assay Biology Blood Cell model Cells Clinical Clinical Research Code Collaborations Communities Cutaneous Data Dermatology Development Disease Drug or chemical Tissue Distribution Engineered Gene Engineered skin Engineering Environment Equipment Exposure to Faculty Flow Cytometry Goals Grant Health Homeostasis Human Hypersensitivity skin testing Image Immune Immune response Immune system Immunologic Monitoring Immunological Models Immunologics Immunology Immunotherapeutic agent In Situ In Vitro Individual Inflammation Innate Immune System Institutional Review Boards Instruction Investigation Laboratories Lead Manuals Monitor Morphology Mus Nanotechnology Nucleotides Pharmaceutical Preparations Preparation Protocols documentation Records Research Research Personnel Research Training Resources Safety Sampling Scientist Secure Services Skin Skin Tissue Technology Testing Tissue Engineering Tissues Translational Research Ultraviolet Rays Universities base biobank design environmental agent enzyme linked immunospot assay equipment training experimental study gene product genetic manipulation graduate student healthy volunteer in vivo in vivo monitoring interest keratinocyte learning materials liquid crystal polymer novel programs reconstitution reconstruction repository sample archive skin disorder tissue archive transcriptome sequencing translational study ultraviolet

3-Dimensional Affect Antibodies Antigens Area Biological Assay Biology Blood Cell model Cells Clinical Clinical Research Code Collaborations Communities Cutaneous Data Dermatology Development Disease Drug or chemical Tissue Distribution Engineered Gene Engineered skin Engineering Environment Equipment Exposure to Faculty Flow Cytometry Goals Grant Health Homeostasis Human Hypersensitivity skin testing Image Immune Immune response Immune system Immunologic Monitoring Immunological Models Immunologics Immunology Immunotherapeutic agent In Situ In Vitro Individual Inflammation Innate Immune System Institutional Review Boards Instruction Investigation Laboratories Lead Manuals Monitor Morphology Mus Nanotechnology Nucleotides Pharmaceutical Preparations Preparation Protocols documentation Records Research Research Personnel Research Training Resources Safety Sampling Scientist Secure Services Skin Skin Tissue Technology Testing Tissue Engineering Tissues Translational Research Ultraviolet Rays Universities base biobank design environmental agent enzyme linked immunospot assay equipment training experimental study gene product genetic manipulation graduate student healthy volunteer in vivo in vivo monitoring interest keratinocyte learning materials liquid crystal polymer novel programs reconstitution reconstruction repository sample archive skin disorder tissue archive transcriptome sequencing translational study ultraviolet

展开

项目摘要

The skin forms a primary interface with the environment, with the keratinocyte a critical component of the innate immune system. Perturbation of healthy skin can lead to inflammation through cutaneous immune responses. As such, the immunology of skin is an important focus of a SBDRC with “The Keratinocyte and its Microenvironment” as its theme. he skin immune system entails an interactive set of innate and adaptive immune cells that can be studied in situ, taken apart for studies at the single cell level, or reconstructed to recreate immune conditions of the skin. The objective of the Translational and Experimental Skin Testing and Immune Tracing (TEST IT) Core is to support and promote the research of SBDRC scientists interested in human and murine cutaneous immune responses in health and disease. The Core will isolate various immune cells from healthy and diseased skin, including at the single cell level, and offer preparative services for the characterization of these cells directly or in functional assays. Using Vectra multispectral imaging equipment, the Core will assist in designing experiments allowing in situ monitoring of human and mouse skin samples, explants and engineered skin. The Core will provide guidance for the efficient expansion of immune cells from skin and blood to be recombined with 3D reconstituted human skin or explants by the Skin Tissue Engineering and Morphology (STEM Core). Prior to reconstruction, cells can be genetically manipulated with products from the Gene Engineering, Transduction and Nanotechnology (GET iN) Core. These engineered keratinocyte-immune cell models will then be available for further immune assessment. A unique and highly novel aspect of the TEST IT core will be to provide a skin challenge facility for in vivo monitoring. This will be accomplished through a dedicated clinical unit and staff to test exposure to environmental agents that affect skin immunologically, whether in a potentially deleterious way, such as ultraviolet/UV light, or in a beneficial way, such as topical medications that suppress immune responses. The Core will oversee the Tissue Acquisition Program (TAP), which obtains archival samples, as well as fresh skin, blood, and other samples (including through the in vivo skin challenge facility) for a central biorepository with secure records; coded, deidentified tissues are distributed to scientists for use in individual laboratories or the service Cores. The Core will also maintain a roster of healthy volunteers and individuals with skin disease who are willing to participate in IRB-approved, skin challenge experiments. These translational studies support mechanistic investigations and provide proof-of-concept data. The Core also focuses on training the research community about immunodermatology, offering expertise in designing mouse and human experiments involving the skin immune system and generating course material in immunodermatology, immunotherapeutics, and immune monitoring. This is a novel core that focuses on enabling investigators to perform advanced and highly translational immune skin research. The ability to study perturbed human skin in a controlled manner will greatly facilitate translational research.

皮肤与环境形成主要界面，角膜细胞是先天的关键组成部分免疫系统。健康皮肤的扰动会通过皮肤免疫反应导致炎症。因此，皮肤的免疫学是SBDRC的重要重点微环境作为主题。他的皮肤免疫系统需要一组互动的先天和适应性免疫可以原位研究的细胞，在单细胞水平上进行研究或重建以重建皮肤的免疫条件。翻译和实验性皮肤测试和免疫的目的跟踪（测试）核心是支持和促进对人类和人类感兴趣的SBDRC科学家的研究鼠健康和疾病中的鼠皮肤免疫反应。核心将使各种免疫细胞与健康且厌恶的皮肤，包括在单细胞水平上，并为表征提供准备的服务这些细胞直接或在功能测定中。使用Vectra多光谱成像设备，核心将有助于在设计实验时，可以原位监测人和老鼠皮肤样品，外植体和设计皮肤。核心将为皮肤和血液中免疫细胞有效扩张提供指导通过皮肤组织工程和形态与3D重建的人皮或外植体重新融合（茎芯）。在重建之前，可以用基因的产物对细胞进行遗传操纵工程，转导和纳米技术（进入）核心。这些设计的角质形成细胞 - 免疫细胞然后，模型将用于进一步的免疫评估。测试的独特且新颖的方面核心将为体内监测提供皮肤挑战设施。这将通过专门的临床部门和工作人员测试暴露于影响皮肤免疫学上的环境药物，无论是以潜在删除的方式，例如紫外线/紫外线，还是以有益的方式，例如局部抑制免疫反应的药物。核心将监督组织采集计划（TAP），获得档案样品以及新鲜的皮肤，血液和其他样品（包括通过体内）皮肤挑战设施）用于具有安全记录的中央生物座席；编码，去识别的组织分布向科学家用于单个实验室或服务核心。核心还将保持健康阵容愿意参加IRB批准的皮肤挑战的志愿者和皮肤病的人实验。这些翻译研究支持机械投资并提供概念证明数据。该核心还专注于培训研究界有关免疫皮肤病学的知识，并提供专业知识设计涉及皮肤免疫系统并生成课程材料的鼠标和人类实验免疫皮肤病学，免疫治疗和免疫监测。这是一个新颖的核心，着重于实现研究人员进行高级和高度翻译的免疫皮肤研究。学习扰动的能力人类皮肤以受控的方式将极大地促进转化研究。