Molecular Analysis of Microbial Pathogens

微生物病原体的分子分析

• 批准号: 10654059
• 负责人: Ralph R. Isberg
• 金额: $ 24.78万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10654059
• 关键词: Molecular Analysis; pathogenic microbe

This application requests support for continuation of a rigorous predoctoral Training Program that focuses on the molecular analysis of microbial pathogens. Experimental research training is the primary focus of this Program. A key and complementary component of the Training Plan is the requirement for Ph.D. students to have exposure to problems in clinical medicine through participation in an intensive summer course in infectious diseases that involves clinical rounds. The training program is designed to provide students with the tools to become independent research scientists in academia or industry while in parallel providing a deep understanding of current problems in clinical infectious diseases as well as training in the important principles of reproducibility and rigor. The Training Program is a track within the interdepartmental Graduate Program in Molecular Microbiology and draws faculty members from the Departments of Molecular Biology and Microbiology, Immunology, Chemistry and Developmental, Molecular & Chemical Biology. All investigators have a common interest in pathogenic microorganisms or their restriction. The varied research interests of the group include: a) bacterial pathogenesis, including the study of colonization, intracellular growth and development of tools to study microbial genes expressed during animal infections; b) viral pathogenesis and replication; c) viral persistence and oncogenesis; d) viral gene expression; e) structure and function of viral entry proteins; f) pathogen evolution during disease; g) antimicrobial resistance; h) development of novel anti-parasitic and anti-fungal strategies; i) protein secretion and the analysis of yeast, parasite and bacterial surfaces; and j) global regulation of gene expression and cell growth in microbial model systems; k) mouse models of innate immunity; and l) microbial interaction with effectors of acquired immunity. The members of this Program use genetic, cell biological, biophysical and biochemical strategies to analyze microbial pathogens, as well as animal infection models. This Training Program has a long history of having a strong collaborative spirit of learning and research among faculty and students. Recruitment and admission strategies have been highly successful, with an excellent minority recruitment program. The overwhelming majority of Ph.D. graduates supported by this Training Program are currently employed in research positions in academics and industry. The Program is overseen by the Training Committee, a group of internationally recognized scientists who participate in the graduate education of all trainees that is subject to external review as well as oversight by a committee that focuses on diversity, equity and inclusion. The application is for five years of support for 5 predoctoral trainee positions per year. Trainees are chosen by a rigorous selection process and are supported, on average, for 2 years, beginning in their second or third year of graduate training.

该申请请求支持继续进行严格的博士前培训计划，该计划 专注于微生物病原体的分子分析。实验研究训练是首要 本计划的重点。培训计划的一个关键和补充组成部分是要求 博士学生通过参加强化课程接触临床医学问题 涉及临床查房的传染病夏季课程。该培训计划旨在 为学生提供成为学术界或工业界独立研究科学家的工具 并行提供对临床传染病当前问题的深入理解以及培训 遵循可重复性和严谨性的重要原则。 培训计划是分子生物学跨部门研究生计划中的一个轨道 微生物学并吸引了来自分子生物学和微生物学系的教员， 免疫学、化学与发育、分子与化学生物学。所有调查员都有一个 对病原微生物或其限制有共同兴趣。不同研究兴趣 组包括：a) 细菌发病机制，包括定植、细胞内生长和 开发研究动物感染期间表达的微生物基因的工具； b) 病毒发病机制和 复制； c) 病毒持久性和肿瘤发生； d) 病毒基因表达； e) 病毒的结构和功能 进入蛋白； f) 疾病期间病原体的进化； g) 抗菌素耐药性； h) 小说的发展 抗寄生虫和抗真菌策略； i) 酵母、寄生虫和细菌的蛋白质分泌和分析 表面； j) 微生物模型系统中基因表达和细胞生长的全局调控； k) 鼠标 先天免疫模型； l) 微生物与获得性免疫效应物的相互作用。成员为 该程序使用遗传、细胞生物学、生物物理和生化策略来分析微生物 病原体以及动物感染模型。该培训计划历史悠久，具有强大的影响力 教师和学生之间的学习和研究的协作精神。招聘及录取 战略非常成功，有出色的少数族裔招募计划。压倒性的 大多数博士。受该培训计划支持的毕业生目前从事研究工作 在学术界和工业界的职位。 该计划由培训委员会监督，该委员会由一群国际公认的科学家组成 参加所有受训人员的研究生教育，并接受外部审查 由一个注重多样性、公平性和包容性的委员会进行监督。申请期限为五年 每年支持 5 个博士前实习生职位。学员是通过严格的选拔流程挑选出来的 从研究生培训的第二年或第三年开始，平均获得两年的支持。

项目成果

Host Shutoff in Influenza A Virus: Many Means to an End.

• DOI: 10.3390/v10090475
• 发表时间: 2018-09-05
• 期刊: Viruses
• 作者: Levene RE;Gaglia MM
• 通讯作者: Gaglia MM

Selective Degradation of Host RNA Polymerase II Transcripts by Influenza A Virus PA-X Host Shutoff Protein.

• DOI: 10.1371/journal.ppat.1005427
• 发表时间: 2016-02
• 期刊: PLoS pathogens
• 影响因子: 6.7
• 作者: Khaperskyy DA;Schmaling S;Larkins-Ford J;McCormick C;Gaglia MM
• 通讯作者: Gaglia MM

Spatial relationships of intra-lesion heterogeneity in Mycobacterium tuberculosis microenvironment, replication status, and drug efficacy.

• DOI: 10.1371/journal.ppat.1010459
• 发表时间: 2022-03
• 期刊: PLoS pathogens
• 影响因子: 6.7
• 作者: Lavin RC;Tan S
• 通讯作者: Tan S

Design principles to assemble drug combinations for effective tuberculosis therapy using interpretable pairwise drug response measurements.

• DOI: 10.1016/j.xcrm.2022.100737
• 发表时间: 2022-09-20
• 期刊: CELL REPORTS MEDICINE
• 影响因子: 14.3
• 作者: Larkins-Ford, Jonah;Degefu, Yonatan N.;Van, Nhi;Sokolov, Artem;Aldridge, Bree B.
• 通讯作者: Aldridge, Bree B.

Morphological profiling of tubercle bacilli identifies drug pathways of action.

结核杆菌的形态学分析可确定药物的作用途径。

• DOI: 10.1073/pnas.2002738117
• 发表时间: 2020
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Smith2nd,TreverC;Pullen,KristaM;Olson,MichaelaC;McNellis,MorganE;Richardson,Ian;Hu,Sophia;Larkins-Ford,Jonah;Wang,Xin;Freundlich,JoelS;Ando,DMichael;Aldridge,BreeB
• 通讯作者: Aldridge,BreeB