Quantitative Proteomics to Develop Robust Senescence-Related Biomarkers for Aging

定量蛋白质组学开发强大的衰老相关生物标志物

• 批准号: 10403934
• 负责人: Birgit Schilling
• 金额: $ 74.16万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10403934
• 关键词: Adopted; Affect; Age; Age-Years; Aging; American; Animals; Atherosclerosis; Baltimore; Biological; Biological Aging; Biological Assay; Biological Markers; Biology of Aging; Cardiovascular Diseases; Cell Aging; Cell Culture Techniques; Cells; Cessation of life; Chronic; Clinical; Complex; Degenerative polyarthritis; Disease; Health; Health Status; Healthcare Systems; Heart; Human; Impaired cognition; Inflammation; Intervention; Kidney Failure; Laboratories; Link; Longitudinal Studies; Malignant Neoplasms; Mass Spectrum Analysis; Measures; Modernization; Molecular; Monitor; Mus; Neurodegenerative Disorders; Normal tissue morphology; Older Population; Organ; Organism; Osteoporosis; Outcome; Pathologic; Pathology; Pathway interactions; Patients; Pharmacologic Substance; Phenotype; Plasma; Proteins; Proteomics; Reproducibility; Research; Risk Factors; Sampling; Societies; Stress; Structure; Technology; Tissues; Transgenic Mice; Work; age related; aged; aging demographic; base; biobank; biomarker panel; candidate marker; candidate validation; cardiovascular health; cell injury; cognitive capacity; cohort; driving force; exhaustion; exosome; falls; frailty; functional decline; functional status; high throughput screening; in vivo; insight; mouse model; multiplex assay; novel; novel marker; pre-clinical; predictive marker; predictive tools; response; response biomarker; sarcopenia; senescence; stem cells; tool; tumorigenesis

SUMMARY Aging entails a plethora of changes at multiple levels – affecting molecules, cells, tissues, organs and integrated organisms -- which culminate in a concerted decline in overall health and ultimately death. Aging is also considered a significant risk factor for many conditions and diseases, such as cardiovascular diseases (CVD), atherosclerosis, sarcopenia, osteoporosis, renal failure and neurodegenerative diseases. Whereas significant progress has been made in understanding several of the cell intrinsic molecular pathways and cellular responses that drive aging, it is very challenging to assess the progression of aging and aging-related conditions, and the field is clearly lacking robust biomarkers for aging. We propose to use our pioneering work in cellular senescence in combination with state-of-the-art proteomics technologies to develop robust senescence-related biomarkers of aging. These novel aging biomarkers are expected to become critical research tools both for transforming the way how to look at human aging and for predicting health outcomes based on biological age and not chronological age (years of age). In parallel, we will refine these biomarkers also for animal studies to gain greater insights into mechanisms of Biology of Aging.

概括 老化需要多个级别的多种变化 - 影响分子，细胞，组织，器官和 综合有机体 - 最终在整体健康和最终死亡的一致下降中达到顶峰。衰老是 在许多疾病和疾病中也被认为是重要的危险因素，例如心血管疾病 （CVD），动脉粥样硬化，肌肉减少症，骨质疏松症，肾衰竭和神经退行性疾病。然而 在理解几个细胞固有的分子途径和 驱动衰老的细胞反应，评估与衰老相关的衰老进展非常挑战 条件，并且该领域显然缺乏衰老的强大生物标志物。我们建议使用我们的开拓性工作 在细胞感应中与最先进的蛋白质组学技术结合使用以发展强大的 衰老的感应相关生物标志物。这些新颖的衰老生物标志物有望变得至关重要 研究工具都可以改变如何看待人类衰老的方式和预测健康结果 基于生物年龄，而不是年代年龄（年龄）。同时，我们将完善这些生物标志物 还可以使动物研究更深入地了解衰老的生物学机制。