HIV Virology Core

HIV病毒学核心

• 批准号: 10653259
• 负责人: Christopher R Aiken
• 金额: $ 85.52万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10653259
• 关键词: Amino Acids; Award; Binding Sites; Biological Assay; CD4 Positive T Lymphocytes; CRISPR/Cas technology; Capsid; Cell Culture Techniques; Cells; Chimeric Proteins; Chromatin; Code; Collaborations; Communities; Complement; Complex; Data; Defect; Development; Engineering; Ensure; Experimental Designs; Genes; Goals; HIV; HIV-1; Histones; Human; Human Resources; In Vitro; Infection; Knock-in; Knock-out; Lentivirus Vector; Maps; Morphology; Mutagenesis; Mutation; Mutation Analysis; Nuclear; Nuclear Import; Nuclear Pore; Proteins; Recombinants; Research; Research Personnel; Resolution; Reverse Transcription; Services; Sheep; Site; Staging; Structure of choroid plexus; T-Lymphocyte; Testing; Transmission Electron Microscopy; Tubular formation; Validation; Viral; Viral Genome; Virion; Virus; Work; cell type; crosslinking and immunoprecipitation sequencing; deep sequencing; experience; experimental study; genetic manipulation; imaging study; integration site; interest; knock-down; knockout gene; live cell imaging; member; mutant; novel; particle; preference; protein complex; protein transport; small molecule inhibitor; tool; trafficking; transcriptional coactivator p75; virology

HIV Virology Core To support the needs of the three PCHPI projects, recipients of Collaborative Development Awards, and external collaborative projects, we will establish an HIV Virology Core (HVC). The primary missons of the HVC will be to test hypotheses resulting from new structural information generated by the center projects and to drive studies that are complementary to the proposed structural work, as detailed in the project descriptions. The HVC will achieve these goals by performing functional studies of HIV-1 using a battery of assays and tools of the trade. Three groups, led by experienced investigators, will contribute to the effort: Chris Aiken (specializing in assays of HIV-1 replication; purification and analysis of HIV-1 cores; functional studies of HIV-1 mutants; and assays of endogenous reverse transcription); Alan Engelman (specializing in functional studies of HIV-1 integration, including deep sequencing analysis of integration sites; gene knock-out and recoding knock-in experiments to anlyze effects of cell mutations on HIV-1 infection, with an emphasis on nuclear entry and integration defects; quantitative assessment of virion morphology by transmission electron microscopy; and work with primary human cell cultures); and Zandrea Ambrose (specializing in single-cycle infection assays; in vitro analysis of single-particle uncoating; staging of HIV-1 infection defects; and cell-based imaging studies of HIV-1 ingress, including intracellular trafficking, gene knockdown/knockout of trafficking proteins, and infections of primary cells). The HVC will be collaboratively managed by Aiken and Engelman, an arrangement that has served the PCHPI well and will ensure that the Projects are appropriately supported.

HIV病毒学核心 为了支持三个PCHPI项目的需求，协作发展奖的获得者以及外部 协作项目，我们将建立HIV病毒学核心（HVC）。 HVC的主要少念将是 由中心项目产生的新结构信息引起的测试假设并推动研究 如项目描述中所详细介绍的拟议结构工作是互补的。 HVC会 通过使用一系列贸易的测定和工具对HIV-1进行功能研究来实现这些目标。 由经验丰富的调查人员领导的三个小组将为这一努力做出贡献：克里斯·艾肯（Chris Aiken）（专门研究测定法 HIV-1复制； HIV-1核的纯化和分析； HIV-1突变体的功能研究；和测定 内源性逆转录）；艾伦·恩格曼（Alan Engelman）（专门研究HIV-1整合功能研究， 包括对整合位点的深入测序分析；基因敲除和重新编码敲门实验 细胞突变对HIV-1感染的ANLYZE影响，重点是核进入和整合缺陷； 通过透射电子显微镜对病毒体形态进行定量评估；并与初级合作 人类细胞培养）；和Zandrea Ambrose（专门研究单周期感染测定；在体外分析 单粒子不涂层； HIV-1感染缺陷的分期；和基于细胞的HIV-1入口研究， 包括细胞内贩运，基因敲低/敲除运输蛋白以及主要感染 细胞）。 HVC将由Aiken和Engelman进行协作，该安排已为 PCHPI良好，将确保项目得到适当的支持。