HIV Virology Core

HIV病毒学核心

基本信息

批准号：
10506951
负责人：
Christopher R Aiken
金额：
$ 83.42万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-01 至 2027-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10506951
关键词：
Amino Acids Award Binding Sites Biological Assay CD4 Positive T Lymphocytes CRISPR/Cas technology Capsid Cell Culture Techniques Cells Chimeric Proteins Chromatin Communities Complement Complex Data Defect Development Engineering Ensure Genes Goals HIV HIV-1 Histones Human Human Resources In Vitro Infection Knock-in Knock-out Lentivirus Vector Maps Morphology Mutagenesis Mutation Mutation Analysis Nuclear Nuclear Import Nuclear Pore Primary Infection Proteins Recombinants Research Research Personnel Resolution Reverse Transcription Services Sheep Site Staging Structure of choroid plexus T-Lymphocyte Testing Transmission Electron Microscopy Tubular formation Validation Viral Viral Genome Virion Virus Work base cell type crosslinking and immunoprecipitation sequencing deep sequencing design experience experimental study genetic manipulation imaging study integration site interest knock-down live cell imaging member mutant novel particle preference protein complex protein transport small molecule inhibitor tool trafficking transcriptional coactivator p75 virology

项目摘要

HIV Virology Core To support the needs of the three PCHPI projects, recipients of Collaborative Development Awards, and external collaborative projects, we will establish an HIV Virology Core (HVC). The primary missons of the HVC will be to test hypotheses resulting from new structural information generated by the center projects and to drive studies that are complementary to the proposed structural work, as detailed in the project descriptions. The HVC will achieve these goals by performing functional studies of HIV-1 using a battery of assays and tools of the trade. Three groups, led by experienced investigators, will contribute to the effort: Chris Aiken (specializing in assays of HIV-1 replication; purification and analysis of HIV-1 cores; functional studies of HIV-1 mutants; and assays of endogenous reverse transcription); Alan Engelman (specializing in functional studies of HIV-1 integration, including deep sequencing analysis of integration sites; gene knock-out and recoding knock-in experiments to anlyze effects of cell mutations on HIV-1 infection, with an emphasis on nuclear entry and integration defects; quantitative assessment of virion morphology by transmission electron microscopy; and work with primary human cell cultures); and Zandrea Ambrose (specializing in single-cycle infection assays; in vitro analysis of single-particle uncoating; staging of HIV-1 infection defects; and cell-based imaging studies of HIV-1 ingress, including intracellular trafficking, gene knockdown/knockout of trafficking proteins, and infections of primary cells). The HVC will be collaboratively managed by Aiken and Engelman, an arrangement that has served the PCHPI well and will ensure that the Projects are appropriately supported.

HIV病毒学核心支持三个 PCHPI 项目、协作开发奖获得者和外部的需求合作项目，我们将建立一个艾滋病病毒学核心（HVC）。 HVC 的主要使命是测试由中心项目生成的新结构信息产生的假设并推动研究这些是对拟议结构工作的补充，如项目说明中详述。 HVC 将通过使用一系列检测方法和行业工具对 HIV-1 进行功能研究来实现这些目标。由经验丰富的研究人员领导的三个小组将为这项工作做出贡献：Chris Aiken（专门从事分析 HIV-1 复制； HIV-1核心的纯化和分析； HIV-1突变体的功能研究；和化验内源性逆转录）； Alan Engelman（专门从事 HIV-1 整合的功能研究，包括整合位点的深度测序分析；基因敲除和重新编码敲入实验分析细胞突变对 HIV-1 感染的影响，重点是核进入和整合缺陷；通过透射电子显微镜定量评估病毒颗粒形态；并与小学一起工作人类细胞培养物）；和 Zandrea Ambrose（专门从事单周期感染测定；体外分析单颗粒脱涂层； HIV-1 感染缺陷的分期；以及 HIV-1 侵入的细胞成像研究，包括细胞内贩运、贩运蛋白的基因敲低/敲除以及原发性感染细胞）。 HVC 将由艾肯和恩格曼合作管理，这一安排已为 PCHPI 很好，并将确保项目得到适当的支持。