Imaging Cells during Behavior Core

行为核心期间的细胞成像

• 批准号: 10634624
• 负责人: TIMOTHY J EBNER
• 金额: $ 38.2万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10634624
• 关键词: 3-Dimensional; Address; Aging; Animals; Behavior; Brain; Brain imaging; Cerebral cortex; Chronic; Cognition; Communities; Complement; Computer software; Data; Decision Making; Development; Disease; Dorsal; Elements; Engineering; Equipment; Failure; Fiber; Free Will; Head; Human Resources; Image; Image Enhancement; Imaging Device; Imaging Techniques; Imaging technology; Individual; Laboratories; Magnetic Resonance Imaging; Magnetism; Methods; Microscope; Minnesota; Modeling; Modernization; Monitor; Movement; Mus; National Institute of Drug Abuse; Nervous System Physiology; Neurons; Neurosciences; Neurosciences Research; Optics; Pattern; Perception; Photometry; Photons; Process; Productivity; Rattus; Relapse; Research; Research Infrastructure; Research Personnel; Residual state; Resolution; Resources; Services; Speed; Structure; Surface; Techniques; Technology; Time; Training; Universities; Visualization; Work; addiction; analytical tool; cellular imaging; computerized tools; cost; design; experimental study; imaging capabilities; imaging modality; improved; in vivo; in vivo imaging; learning network; manufacture; miniaturize; mouse model; nervous system imaging; neural; neural circuit; neuroimaging; novel therapeutics; optical imaging; programs; spatiotemporal; tool

PROJECT SUMMARY: Imaging Cells during Behavior Core Addiction is a chronic, relapsing disorder due to perturbations in neural circuits. To better understand the underlying pathophysiological processes occurring in addiction and the actions of new therapies, there are pressing needs to monitor neural activity and structure during addiction and relapse. Visualization of nervous system function and structure in the intact, behaving animal is a powerful approach to meet these needs. Recent advances in genetically encoded Ca2+ indicators and imaging instruments have greatly expanded the opportunities for imaging the nervous system at work. Concurrently, there is a revolution in the use of high-speed video cameras combined with sophisticated computational tools to monitor and quantify behavior. Many labs at the University of Minnesota (UMN) lack the equipment and expertise to perform neural imaging during behavior. Nor is it possible for most investigators to keep up with the rapid changes in optical tools, either in the engineering or application domains. The Imaging Cells during Behavior Core (ICBC) is designed to allow addiction researchers and the wider neuroscience community access to modern techniques to image brain function and structure during behavior. Providing up-to-date imaging techniques, engineering and manufacturing support, as well as computational tools, will not only reduce the time and costs needed to set up experiments and process data, but also lower the entry barriers for both new and established investigators. The ICBC’s engineering, experimental, and analytical expertise will allow labs to be at the forefront of imaging technology. Aim 1 is to provide resources, expertise and training in three in vivo imaging technologies used in behaving animals: 1) head-mounted miniaturized microscopes for cellular level imaging at the surface and in deeper brain structures; 2) wide field-of-view (FOV) imaging of the cerebral cortex at both the mesoscopic and cellular levels; and 3) fiber photometry to monitor the activity of genetically defined elements in neural circuits. UMN investigators deemed these technologies critical to understanding how neural circuits change in addiction. The ICBC will provide sophisticated hardware and software to monitor and analyze behavior in head-fixed and freely moving animals and the engineering and analytical expertise to build and use these in vivo imaging technologies. Aim 2 extends and adds new imaging capabilities. Many UMN addiction researchers use rats, but most neural imaging techniques were developed for mice. Therefore, we will extend our imaging tools to the rat. In addition, Aim 2 will combine fiber photometry with wide FOV cortical imaging, enhance wide FOV imaging to access deeper cortical structures, and make wide FOV imaging compatible with magnetic resonance imaging. ICBC personnel will work closely with investigators to employ these improvements to advance their research programs. In summary, the ICBC will provide the research infrastructure, expertise and training to allow neuroscientists to image neuronal activity and neural circuitry during behavior. Providing these tools will enhance the productivity, quality, and impact of addiction-related and neuroscience research in general at the UMN.

项目摘要：行为核心期间的细胞成像 成瘾是一种由于神经回路扰动而导致的慢性、复发性疾病。 成瘾中发生的潜在病理生理过程和新疗法的作用，有 迫切需要监测成瘾和复发期间的神经活动和结构。 完整的、行为良好的动物的系统功能和结构是满足这些需求的有力方法。 基因编码 Ca2+ 指示剂和成像仪器的进步极大地扩展了 同时，高速的使用也发生了一场革命。 摄像机与复杂的计算工具相结合来监控和量化行为。 明尼苏达大学 (UMN) 的许多实验室缺乏进行神经成像的设备和专业知识 大多数研究人员也不可能跟上光学工具的快速变化。 行为核心 (ICBC) 期间的成像细胞旨在允许在工程或应用领域中使用。 成瘾研究人员和更广泛的神经科学界可以利用现代技术对大脑进行成像 提供最新的成像技术、工程和制造。 支持以及计算工具不仅会减少设置实验所需的时间和成本 和处理数据，同时也降低了新研究者和老研究者的进入门槛。 工程、实验和分析专业知识将使实验室处于成像技术的最前沿。 目标 1 是提供用于行为的三种体内成像技术的资源、专业知识和培训 动物：1) 头戴式微型显微镜，用于大脑表面和深层细胞水平成像 2）在介观和细胞水平上对大脑皮层进行宽视场（FOV）成像； 3) 光纤光度法监测 UMN 中基因定义的元素的活动。 研究人员认为这些技术对于理解成瘾过程中神经回路如何变化至关重要。 ICBC将提供先进的硬件和软件来监控和分析头部固定和自由的行为 移动动物以及构建和使用这些体内成像技术的工程和分析专业知识。 Aim 2 扩展并增加了新的成像功能，许多 UMN 成瘾研究人员使用大鼠，但大多数神经网络。 因此，我们将我们的成像工具扩展到大鼠。 Aim 2 将光纤光度测量与宽视场皮层成像相结合，增强宽视场成像以访问 更深的皮质结构，并使宽视场成像与磁共振成像兼容。 工作人员将与研究人员密切合作，利用这些改进来推进他们的研究计划。 总之，ICBC 将提供研究基础设施、专业知识和培训，使神经科学家能够 提供这些工具将提高生产力， UMN 的成瘾相关研究和神经科学研究的质量和影响。