Central mechanisms for integrating distinct retinal inputs during the optokinetic reflex in mice

小鼠视动反射过程中整合不同视网膜输入的中心机制

基本信息

批准号：
10471199
负责人：
Scott C Harris
金额：
$ 4.04万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10471199
关键词：
Algorithms Amblyopia Anatomy Animals Architecture Automobile Driving Axon Behavior Behavioral California Cell Nucleus Cells Classification Complement Cues Data Development Diagnosis Dorsal Elements Environment Eye Eye Movements Goals Head Image In Vitro Interneurons Knowledge Label Lateral Life Measures Medial Mentorship Modeling Motion Motor Movement Mus Nature Neural Pathways Neuraxis Neurons Ocular Motility Disorders Organism Output Pathologic Nystagmus Pathway interactions Pattern Physiology Population Positioning Attribute Process Property Reflex action Research Training Resources Retina Retinal Ganglion Cells Role San Francisco Sensory Signal Transduction Source Speed Stimulus Strabismus Stream Techniques Testing Training Transgenic Organisms Translating Universities Vision Visual Pathways Whole-Cell Recordings Work awake career development cell type extracellular in vivo innovation insight novel strategies relating to nervous system response signal processing visual information

项目摘要

PROJECT SUMMARY The optokinetic reflex (OKR) is an innate, visually-driven behavior that is critical to normal vision. During OKR, compensatory eye movements stabilize vision in response to slow, global image motion that often results from changes in head position. While OKR is robust across diverse species, its underlying mechanisms are unknown. This project aims to understand how distinct retinal output streams that collectively encode OKR-inducing stimuli are centrally integrated to drive eye movements. To achieve this goal, a combination of physiology and behavioral techniques will be used to measure functional readouts from three stages along the OKR pathway of the mouse in response to a common stimulus set: 1) The population activity of retinal ganglion cells that are responsible for detecting and encoding OKR-inducing stimuli, 2) Downstream neurons in the medial terminal nucleus – a primary central hub for OKR processing, and 3) The eye movements of awake animals as they perform OKR. This novel approach to describing signal transformation along the entirety of a visual pathway will provide a mapping of retinal activity onto behavior and offer insight into how and where information from distinct retinal output streams is centrally integrated. Broadly, such findings are important for revealing general strategies by which elements of the central nervous system integrate competing inputs from distinct sources. Further, revealing fundamental features of eye movement pathways will make progress towards understanding the mechanisms behind a variety of common eye movement disorders including nystagmus, strabismus, and amblyopia. This work will be complemented by a rigorous training plan that involves tailored mentorship, coursework, presentation opportunities, and career development. Along with the institutional, intellectual, and training resources available in the highly collaborative scientific environment at the University of California, San Francisco, these research and training plans will jointly facilitate the applicant’s development into an independent sensory neuroscientist.

项目概要视动反射 (OKR) 是一种天生的、视觉驱动的行为，在 OKR 过程中对正常视力至关重要。代偿性眼球运动稳定视力，以响应缓慢的全局图像运动，这通常是由虽然 OKR 在不同物种中都很有效，但其潜在机制尚不清楚。该项目旨在了解共同编码 OKR 诱导刺激的不同视网膜输出流集中整合以驱动眼球运动，以实现这一目标，需要结合生理学和行为技术将用于测量 OKR 路径中三个阶段的功能读数小鼠对常见刺激组的反应：1）视网膜神经节细胞的群体活动负责检测和编码 OKR 诱导刺激，2) 内侧末端的下游神经元细胞核 – OKR 处理的主要中心枢纽，以及 3) 清醒动物的眼球运动这种描述整个视觉路径信号转换的新颖方法将执行 OKR。提供视网膜活动到行为的映射，并深入了解不同信息的方式和位置从广义上讲，这些发现对于揭示一般策略很重要。中枢神经系统的各个要素通过这些要素整合来自不同来源的竞争性输入。揭示眼球运动路径的基本特征将有助于理解各种常见眼球运动障碍背后的机制，包括眼球震颤、斜视和这项工作将得到严格的培训计划的补充，其中包括量身定制的指导，课程作业、演讲机会和职业发展以及机构、智力和发展。加州大学圣保罗分校高度协作的科学环境中提供的培训资源 Francisco先生，这些研究和培训计划将共同促进申请人发展成为独立的感觉神经科学家。