Central mechanisms for integrating distinct retinal inputs during the optokinetic reflex in mice

小鼠视动反射过程中整合不同视网膜输入的中心机制

基本信息

批准号：
10315952
负责人：
Scott C Harris
金额：
$ 3.96万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10315952
关键词：
Algorithms Amblyopia Anatomy Animals Architecture Automobile Driving Axon Behavior Behavioral California Cell Nucleus Cells Classification Complement Cues Data Development Diagnosis Dorsal Elements Environment Eye Eye Movements Goals Head Image In Vitro Interneurons Knowledge Label Lateral Life Measures Medial Mentorship Modeling Motion Motor Movement Mus Nature Neural Pathways Neuraxis Neurons Ocular Motility Disorders Organism Output Pathologic Nystagmus Pathway interactions Pattern Physiology Population Positioning Attribute Process Property Reflex action Research Training Resources Retina Retinal Ganglion Cells Role San Francisco Sensory Signal Transduction Source Speed Stimulus Strabismus Stream Techniques Testing Training Transgenic Organisms Translating Universities Vision Visual Pathways Whole-Cell Recordings Work awake career development cell type extracellular in vivo innovation insight novel strategies relating to nervous system response signal processing visual information

项目摘要

PROJECT SUMMARY The optokinetic reflex (OKR) is an innate, visually-driven behavior that is critical to normal vision. During OKR, compensatory eye movements stabilize vision in response to slow, global image motion that often results from changes in head position. While OKR is robust across diverse species, its underlying mechanisms are unknown. This project aims to understand how distinct retinal output streams that collectively encode OKR-inducing stimuli are centrally integrated to drive eye movements. To achieve this goal, a combination of physiology and behavioral techniques will be used to measure functional readouts from three stages along the OKR pathway of the mouse in response to a common stimulus set: 1) The population activity of retinal ganglion cells that are responsible for detecting and encoding OKR-inducing stimuli, 2) Downstream neurons in the medial terminal nucleus – a primary central hub for OKR processing, and 3) The eye movements of awake animals as they perform OKR. This novel approach to describing signal transformation along the entirety of a visual pathway will provide a mapping of retinal activity onto behavior and offer insight into how and where information from distinct retinal output streams is centrally integrated. Broadly, such findings are important for revealing general strategies by which elements of the central nervous system integrate competing inputs from distinct sources. Further, revealing fundamental features of eye movement pathways will make progress towards understanding the mechanisms behind a variety of common eye movement disorders including nystagmus, strabismus, and amblyopia. This work will be complemented by a rigorous training plan that involves tailored mentorship, coursework, presentation opportunities, and career development. Along with the institutional, intellectual, and training resources available in the highly collaborative scientific environment at the University of California, San Francisco, these research and training plans will jointly facilitate the applicant’s development into an independent sensory neuroscientist.

项目摘要光动力学反射（OKR）是一种先天的，视觉驱动的行为，对正常视力至关重要。在OKR期间，补偿性眼动动作稳定视力，以响应缓慢的全球图像运动，通常是由头部位置变化。虽然OKR在潜水员的种类中都有坚固的态度，但其潜在机制尚不清楚。该项目旨在了解如何集体编码OKR诱导OKR的Stimulli的不同视网膜输出流被集中整合以驱动眼睛运动。为了实现这一目标，生理学和行为技术将用于从OKR途径的三个阶段测量功能读数响应常见刺激集的小鼠：1）残留神经节细胞的种群活性负责检测和编码OKR诱导刺激，2）媒体终端中的下游神经元细胞核 - OKR加工的主要中心枢纽，3）清醒动物的眼睛运动执行OKR。这种描述沿整个视觉途径的信号转换的新颖方法将将视网膜活动映射到行为上，并深入了解如何和何处。视网膜输出流是集中集成的。从广义上讲，此类发现对于揭示一般策略很重要中枢神经系统的要素通过不同来源整合了竞争的投入。此外，揭示眼动路径的基本特征将使了解各种常见的眼动障碍背后的机制，包括眼球震颤，斜视和弱视。这项工作将由严格的培训计划完成，该计划涉及量身定制的Mentalship，课程工作，演讲机会和职业发展。以及制度，智力和培训资源可在加利福尼亚大学SAN的高度协作科学环境中获得弗朗西斯科，这些研究和培训计划将共同支持申请人的发展感觉神经科学家。