Rapid Characterization of the Vaginal Microbiome/Virome in Pregnant Women

孕妇阴道微生物组/病毒组的快速表征

• 批准号: 10368148
• 负责人: Kristine M. Wylie
• 金额: $ 7.88万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-08 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10368148
• 关键词: 37 weeks gestation; Affect; Bacteria; Biological Assay; Biological Markers; Birth; Characteristics; Clinical; Clinical Research; Communities; Consumption; Cultured Cells; DNA; Data; Data Set; Detection; Development; Disease; Eukaryota; Fibrinogen; First Pregnancy Trimester; Follow-Up Studies; Foundations; Future; Gardnerella vaginalis; High-Throughput Nucleotide Sequencing; Individual; Microbe; Neonatal Mortality; Nucleic Acids; Papillomavirus; Pregnant Women; Premature Birth; Publishing; RNA; Resources; Risk; Sampling; Sensitivity and Specificity; Swab; Term Birth; Testing; Time; Trichomonas vaginalis; United States; Vagina; Viral; Virus; Work; assay development; bacterial community; base; cost; cost effective; design; human female; innovation; interest; metagenomic sequencing; microbial; microbial community; microbial signature; microbiome analysis; microbiome research; multiplex detection; nano-string; neonatal morbidity; prevent; reproductive tract; success; vaginal microbiome; vaginal microbiota; virome

ABSTRACT Preterm birth (PTB), delivery occurring before 37 weeks of gestation, affects approximately 10% of births in the United States each year and is the primary cause of neonatal morbidities and mortality. Because our understanding of the causes of PTB is incomplete, we are limited in our ability to predict or prevent PTB. In our previous studies, we used high-throughput sequencing to reveal characteristics of viral and bacterial communities in the vagina are associated with PTB. These data suggest that characteristics of the vaginal microbiome may serve as biomarkers for PTB, although more studies are required. While sequencing assays have been useful in beginning to identify microbial signatures associated with PTB, the incorporation of these assays into large-scale follow up studies is limited by several factors: (i) sequencing assays are in many cases qualitative, requiring subsequent assays to quantitate the microbes, thereby depleting sample material; (ii) sequencing and qPCR assays are time-consuming, requiring weeks to obtain complete data sets from each sample; and (iii) sequencing assays are relatively expensive despite many innovations that have reduced costs. Based on our increased understanding of vaginal microbial community features associated with PTB, we presently aim to develop a single, multiplexed, targeted assay that will allow us to capture the essential features we find in sequencing assays, but it will do so more quantitatively, rapidly, and cost-effectively than sequencing. The development of this assay will allow us (and others who study the microbiome of the human female reproductive tract) to maximize resource utilization in future studies by replacing multiple sequencing and PCR tests with a single, quantitative, rapid, easy to use, less expensive assay. Furthermore, this assay may serve as a foundation for future clinical studies aimed at defining risk for PTB in pregnant women

抽象的 早产（PTB）发生在妊娠37周之前的分娩，影响了大约10％的出生 美国每年是新生儿病毒和死亡率的主要原因。因为我们 了解PTB的原因是不完整的，我们的预测或预测PTB的能力受到限制。在我们的 先前的研究，我们使用高通量测序来揭示病毒和细菌的特征 阴道中的社区与PTB有关。这些数据表明阴道的特征 微生物组可以作为PTB的生物标志物，尽管需要更多的研究。同时进行测定 对于开始识别与PTB相关的微生物特征非常有用 大规模后续研究的测定受到多个因素的限制：（i）在许多情况下进行测序测定 定性，需要随后的测定来定量微生物，从而耗尽样品材料； （ii） 测序和QPCR分析是耗时的，需要数周从每个 样本; （iii）测序测定相对昂贵，尽管许多创新降低了成本。 根据我们对与PTB相关的阴道微生物社区特征的了解，我们 目前旨在开发单个，多重的，有针对性的测定法，这将使我们能够捕获基本特征 我们在测序测定中发现，但是比测序更定量，快速和成本效益。 该测定法的开发将使我们（以及研究人类女性微生物组的其他人 生殖道）通过替换多个测序和PCR来最大化资源利用率 进行单个，定量，快速，易于使用的测试，价格较便宜。此外，该测定法可能是 旨在确定孕妇PTB风险的未来临床研究的基础