Embryonic stem cell/induced Pluripotent stem cell growth and gene editing core

胚胎干细胞/诱导多能干细胞生长和基因编辑核心

基本信息

批准号：
10495947
负责人：
Lei Yang
金额：
$ 51.94万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-02-15 至 2028-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10495947
关键词：
Animal Experiments Animal Model Animals Applications Grants Breeding Cardiac Cell model Cell physiology Cells Cellular Structures Clone Cells Clustered Regularly Interspaced Short Palindromic Repeats Cryopreservation Development Disease ES Cell Line Embryo Etiology Experimental Animal Model Founder Generation Gene Targeting Gene Transfer Techniques Generations Genes Genetic Genetic Models Genotype Goals Growth Human Human Resources In Vitro Indiana Injections Modeling Modification Molecular Morphogenesis Mus Mutate Mycoplasma Participant Performance Program Research Project Grants Protocols documentation Quality Control Reagent Regulation Services Technology Testing Training Transgenic Mice Universities Ventricular blastocyst cardiogenesis congenital heart disorder cost design embryo culture embryonic stem cell experience experimental study genetic manipulation genome editing human embryonic stem cell in vivo in vivo Model induced pluripotent stem cell medical schools mouse genetics mouse model pluripotency programs recruit screening stem cell growth stem cells success transmission process

项目摘要

ABSTRACT The Embryonic Stem Cell Growth and Genetically Modified Models Core (Core B) will provide service to all four Projects with the goal of efficiently generating and providing cellular and animal experimental models of congenital heart disease (CHD) to Program participants. Aim 1 will provide human and mouse embryonic stem cell (hESC and mESC)-derived cellular models of CHD to the projects. This will include (1a) providing support for genome editing of hESC and mESCs, (1b) maintaining high quality stocks of existing and newly generated hESC and mESC lines used by each project, and (1c) providing assistance with hESC and mESC differentiation towards cardiac lineages. Aim 2 will provide mouse genetic models of CHD to the projects. This will include (2a) providing support for the generation of new genetically modified mouse models, (2b) establishing and maintaining breeding stocks for new and existing mouse models to be used by the Projects, and (2c) providing experimental animals for each Project. Core B personnel are well-trained in all aspects of the cellular and animal services to be provided. The goal of the Core is to provide centralized, quality-controlled reagents to the Projects, thereby maximizing efficiency and minimizing the costs associated with the usage of cellular and animal models. Given the heavy reliance on genetically modified models (both cellular and animal) and the proven performance of the Core personnel, the importance of Core B to the success of this Program Project Grant application cannot be overstated.

抽象的胚胎干细胞生长和转基因模型核心（核心B）将为所有四个提供服务项目的目标是有效地生成和提供细胞和动物实验模型为参与者提供的先天性心脏病（CHD）。 AIM 1将提供人类和小鼠胚胎茎细胞（HESC和MESC）衍生的CHD的细胞模型。这将包括（1A）提供支持对于hESC和MESC的基因组编辑，（1B）维持现有和新生成的高质量库存每个项目使用的HESC和MESC线，以及（1C）提供hESC和MESC差异的帮助朝向心脏谱系。 AIM 2将为项目提供CHD的鼠标遗传模型。这将包括（2a）为生成新的转基因鼠标模型（2b）建立和维护项目使用新的和现有的鼠标模型的育种库存，（2C）提供每个项目的实验动物。核心B人员在细胞和动物的各个方面都有良好的训练提供的服务。核心的目的是为项目提供集中的，质量控制的试剂，从而最大程度地提高效率并最大程度地减少与细胞和动物模型使用相关的成本。鉴于对转基因模型（包括细胞和动物）的严重依赖，并且具有验证的性能在核心人员中，核心B对此计划项目授予应用程序的成功的重要性不能被夸大了。