Research Supplements to Promote Diversity in Health-Related Research

促进健康相关研究多样性的研究补充

• 批准号: 10356271
• 负责人: Nan Gao
• 金额: $ 4万
• 依托单位: RUTGERS THE STATE UNIV OF NJ NEWARK
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10356271
• 关键词: Administrative Supplement; Award; Biochemical; Biology; Cell Wall; Cells; Cleaved cell; Clinical; Colitis; Communication; Crohn' s disease; Defect; Doctor of Philosophy; Funding; Genetic; Glycoside Hydrolases; Gnotobiotic; Goals; Gut Mucosa; Health; Homeostasis; Immunity; Immunology; Infection; Inflammatory Response; Intestines; Investigation; Knowledge; Laboratories; Laboratory Research; Literature; Malignant Neoplasms; Mentors; Minority; Motivation; Mucosal Immune Responses; Mucous Membrane; Muramidase; Mus; Nature; Paneth Cells; Paper; Parents; Pathogenicity; Pathway interactions; Patients; Physiological; Physiology; Production; Productivity; Publications; Publishing; Regulation; Research; Role; Science; Time; Training; Universities; antimicrobial peptide; bactericide; career; career development; cohesion; commensal microbes; experimental study; frontier; gut microbiota; inflammatory disease of the intestine; insight; intestinal epithelium; microbiota; mouse model; multidisciplinary; novel; parent grant; parent project; pre-doctoral; prevent; response to injury; skills; trafficking

PROJECT SUMMARY This is an administrative supplement application that aims to promote the research productivity and career training of a promising minority pre-doctoral fellow. The hypothesis was built on a new observation made by the candidate in terms of how Paneth cell lysozyme secretion may be regulated. The proposed experiments will biochemically quantify lysozyme secretion from mouse intestinal lumen of mice lacking Rab11b trafficking. Additional proposed experiments will uncover Paneth cell specific Rab11b interactome, which may shed lights on novel regulatory mechanism for antimicrobial peptide secretion. The project will enhance and expand the overarching goal of the parent award that investigates contribution of intestinal lysozyme to mucosal immune response to commensal microbiota. The candidate is currently a full-time predoctoral fellow in the PI’s laboratory at Rutgers University. The candidate’s academic path in the last ten years convincingly demonstrated a strong motivation and persistent endeavor in pursuing laboratory research and biomedical science as a career. The project is important as elevated levels of intestinal lysozyme and its ectopic production by colonic mucosa are observed in clinically active intestinal inflammation conditions. A better understanding of how lysozyme secretion is regulated will bring insights into the frequently observed defective lysozyme packaging and secretion in Paneth cells of Crohn’s disease (CD) patients. The project is multidisciplinary in nature and relies on different expertise in experimental colitis, mucosal immunology, gnotobiotics study, genetics, and intestinal epithelial biology. Thus, the project is ideal for training of a Ph.D. candidate as it requires strong collaborative activities and scientific communication skills. Career development and mentoring plans are cohesively put in place to facilitate a successful completion of the Ph.D. dissertation and a transition into postdoctoral research by the candidate.

项目摘要 这是一个行政补充应用，旨在促进研究生产率和 承诺少数派前博士学位的职业培训。该假设建立在新的 候选人对paneth细胞溶菌酶分泌的观察结果可能受到调节。 提出的实验将在生化中量化小鼠肠腔内的溶菌酶分泌 缺乏Rab11b贩运的小鼠。其他提出的实验将发现Paneth细胞特异性 Rab11b Interactome，它可能会在抗菌胡椒的新型调节机制上阐明灯光 分泌。该项目将增强和扩大父母奖的总体目标 研究了肠道溶菌酶对粘膜免疫反应对共生微生物群的贡献。 该候选人目前是罗格斯大学（Rutgers University）PI实验室的全职专家。这 在过去十年中，候选人的学术道路令人信服地表现出强烈的动力和 持续努力追求实验室研究和生物医学科学作为职业。项目 很重要，因为肠道溶菌酶的水平升高及其由结肠粘膜产生的生产 在临床活跃的肠道感染条件下观察到。更好地了解溶菌酶 分泌受到调节，将使经常观察到的有缺陷的溶菌酶包装和 克罗恩氏病（CD）患者的潘特细胞中的分泌。该项目本质上是多学科的 依靠实验性结肠炎，粘膜免疫学，gnotobiotics研究，遗传学， 和肠上皮生物学。这是该项目是培训博士学位的理想选择。候选人需要 强大的协作活动和科学沟通技巧。职业发展和心理化 制定了计划，以促进成功完成博士学位。论文和a 候选人过渡到博士后研究。