Administrative Supplements for Equipment Purchases

设备采购行政补充

• 批准号: 10794680
• 负责人: Mi Hye Song
• 金额: $ 9.99万
• 依托单位: OAKLAND UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10794680
• 关键词: 26S proteasome; Administrative Supplement; Affect; Award; Biochemical; Biological Models; Biology; Brain; CRISPR/Cas technology; Caenorhabditis elegans; Cell Cycle; Cell division; Centrioles; Centrosome; Compensation; Defect; Detection; Development; Disease; Embryo; Ensure; Equipment; Exhibits; Funding; Genetic; Genomic Instability; Goals; Healthcare; Homeostasis; Human; Knowledge; Malignant Neoplasms; Measurement; Mediating; Microcephaly; Microtubule-Organizing Center; Mus; Mutation; National Institute of General Medical Sciences; Parents; Predisposition; Protein Analysis; Proteins; Proteolysis; Quantitative Microscopy; Regulation of Proteolysis; Request for Proposals; Research; Resolution; Role; Signal Transduction; System; Testing; Therapeutic Intervention; Work; anaphase-promoting complex; ciliopathy; daughter cell; equipment acquisition; genome editing; genome integrity; human disease; imaging system; in vivo; in vivo Model; mutant; nano; parent project; recruit; success; transmission process; tumorigenesis; ubiquitin ligase; ubiquitin-protein ligase

Project Summary Under the current NIGMS R15 research award (1R15GM147857-01), this proposal requests supplemental equipment funds that will be used to replace an old broken Odyssey Infrared Imaging system. The parent project focuses on the Proteolytic Regulation of Centrosome Proteins. The requested equipment is essential for carrying out the experimental plans for the Quantitative and Qualitative analysis of proteins successfully. The accurate measurement of protein levels and detection of proteins in low abundance require enhanced sensitivity and resolution. This administrative supplement fund will allow us to purchase Odyssey M Imaging system. This new imaging system will replace a broken Odyssey Infrared scanner and offer technical upgrades in the sensitivity and resolution of signal detection, which will serve a critical role in the success of the parent R15 project.

项目摘要 根据当前的Nigms R15研究奖（15GM147857-01），该提案请求 补充设备资金将用于替换旧的破碎奥德赛红外线 成像系统。家长项目侧重于中心体的蛋白水解调节 蛋白质。请求的设备对于执行实验计划至关重要 蛋白质的定量和定性分析成功。准确 蛋白质水平的测量和低丰度中蛋白质的检测需要增强 灵敏度和分辨率。该行政补充基金将允许我们购买奥德赛 M成像系统。这个新的成像系统将取代损坏的奥德赛红外扫描仪 并提供有关信号检测的灵敏度和分辨率的技术升级，这将服务 在父级R15项目的成功中起着关键作用。

项目成果

Site-specific phosphorylation of ZYG-1 regulates ZYG-1 stability and centrosome number.

• DOI: 10.1016/j.isci.2023.108410
• 发表时间: 2023-12-15
• 期刊: ISCIENCE
• 影响因子: 5.8
• 作者: Medley, Jeffrey C.;Yim, Rachel N.;DiPanni, Joseph;Sebou, Brandon;Shaffou, Blake;Cramer, Evan;Wu, Colin;Kabara, Megan;Song, Mi Hye
• 通讯作者: Song, Mi Hye

kin-3 genetically suppresses sur-6 in centrosome assembly during Caenorhabditis elegans embryogenesis.

• DOI: 10.17912/micropub.biology.000791
• 发表时间: 2023
• 期刊: microPublication biology
• 作者: Medley, Jeffrey C;Song, Mi Hye
• 通讯作者: Song, Mi Hye