Integrative Analysis of Wilson's Disease

威尔逊病的综合分析

• 批准号: 9699645
• 负责人: SVETLANA LUTSENKO
• 金额: $ 12万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2021-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9699645
• 关键词: Cell Compartmentation; Cell Density; Cells; Environment; Evaluation; Grant; Hepatolenticular Degeneration; Kinetics; Manufacturer Name; Measurement; Microscope; Morphology; Norepinephrine; Oxidation-Reduction; Price; Regulation; Research; Signaling Molecule; Speed; Tissues; Wilson disease protein; discount; improved; instrument; live cell imaging; trafficking

PROJECT SUMMARY The LSM800 instrument will be heavily used for all specific aims of the parental grant. The studies that will be performed with the microscope include analysis of trafficking of ATP7A and ATP7B between the cell compartments, regulation of the trafficking by norepinephrine and other signaling molecules, intracellular measurements of coper transport activity, studies of the redox environment in live cells, and evaluation of tissue morphology with a quantitative analysis of cell densities. Studies in live cells would allow more sophisticated kinetic measurements as well as evaluation of spatial aspects of DBH secretion, which has not been done previously. The ability to re-use several components of the Pascal microscope, which is no longer supported by the manufacturer, resulted in a significant discount in the price of the microscope, despite addition of the live-cell imaging option, which is highly beneficial for the proposed research.

项目概要 LSM800 工具将大量用于家长补助金的所有具体目标。研究 将使用显微镜进行的分析包括 ATP7A 和 ATP7B 的运输分析 细胞区室之间的去甲肾上腺素和其他信号传导调节运输 分子、铜转运活性的细胞内测量、氧化还原研究 活细胞环境，以及通过细胞定量分析评估组织形态 密度。对活细胞的研究将允许更复杂的动力学测量以及 评估胸径分泌的空间方面，这是以前从未做过的。能力 重新使用帕斯卡显微镜的多个组件，该组件不再受支持 制造商，导致显微镜价格大幅折扣，尽管添加了 活细胞成像选项，这对拟议的研究非常有益。