CORE--CELL CULTURE

核心--细胞培养

• 批准号: 3737210
• 负责人: JAMES R YANKASKAS
• 金额: --
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3737210
• 关键词: animal tissue; biomedical facility; cystic fibrosis; human tissue; patient /disease registry; respiratory epithelium; tissue /cell culture; tissue /cell preparation; tissue resource /registry

The Cell Culture Core supports Gene Therapy for Cystic Fibrosis investigators by supplying cultured epithelial cells and cell lines from humans and animals in conditions appropriate to the planned experiments. This overall goal is achieved through four Specific Aims: 1) tissue acquisition; 2) patient characterization; 3) primary cell culture; and 4) cell line development and culture. Normal and cystic fibrosis (CF) patients scheduled for elective nasal or lung surgery are identified and characterized by established clinical and laboratory criteria. Epithelial cells are isolated from excess excised tissues and cultured by established techniques that preserve the physiological properties that are essential to the proposed studies. Specialized cell purification and culture techniques are used as needed to achieve specific experimental goals. Canine and mouse [normal or cftr(-/-)] airway epithelial cells are isolated and cultured by analogous techniques. Epithelial cell lines that express the essential characteristics of the native tissue are developed or obtained, and provided to PPG investigators as needed to meet overall experimental goals. These activities reflect the centralized provision of established techniques that have successfully supported CF research for more than 9 years, and the adaptation of newer reagents to these goals.

细胞培养核心支持囊性纤维化的基因治疗 研究人员通过提供培养的上皮细胞和细胞系 人类和动物在适合计划实验的条件下进行。 这一总体目标是通过四个具体目标实现的：1) 组织 获得; 2）患者特征； 3）原代细胞培养；和 4) 细胞系开发和培养。正常和囊性纤维化 (CF) 确定计划进行选择性鼻或肺手术的患者，并 以既定的临床和实验室标准为特征。上皮细胞 从多余的切除组织中分离细胞并通过建立的方法进行培养 保留必要的生理特性的技术 到拟议的研究。专门的细胞纯化和培养 根据需要使用技术来实现特定的实验目标。 犬和​​小鼠 [正常或 cftr(-/-)] 气道上皮细胞 通过类似技术分离和培养。上皮细胞系 表达天然组织的基本特征已发展 或根据需要获得并提供给 PPG 调查人员，以满足总体要求 实验目标。这些活动反映了集中提供 已建立的技术已成功支持 CF 研究 超过 9 年的时间，以及更新的试剂适应这些目标。