CORE--TISSUE CULTURE

核心——组织培养

• 批准号: 6353095
• 负责人: JAMES R YANKASKAS
• 金额: $ 22.26万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6353095
• 关键词: biomedical facility; cystic fibrosis; respiratory epithelium; tissue /cell culture

Epithelial cell cultures are instrumental in defining the pathophysiology of CF. A Tissue Procurement and Cell Culture Core was established at the University of North Carolina (UNC) in 1984, under the auspices of the Cystic Fibrosis Foundation, to provide standardized cell cultures to CF researchers. The Core has supported projects of the UNC Cystic Fibrosis Research Center since 1988, and has increased its output and capabilities to meet growing research demands. The Tissue Culture Core will support the SCOR projects through three major functions. 1. Tissue procurement. . Obtain nasal and lung tissue from normal, disease control, and cystic fibrosis humans as sources of airway epithelial cells. . Characterize donors with respect to diagnosis, demographic, and clinical features essential to research uses. 2. Airway epithelial cell isolation and culture. . Isolate epithelial cells from large and small airways of human, primate and rodent lungs. . Prepare and maintain primary epithelial cell cultures from define airway regions. . Prepare differentiated cultures from passaged, or cryopreserved and thawed cells on substrates as dictated by investigator needs. . Prepared and provide airway epithelial cell lines from gene knockout mice. 3. Cell culture technical development. . Optimize epithelial culture conditions to replicate the gene expression, morphological differentiation, and ion and mucus secretory functions of normal and CF large and small airways. . Produce genetically manipulated, well-differentiated primary airway epithelial cell cultures. . Provide very high humidity culture environments needed to assess airway surface liquid topography in the absence of evaporation artifacts.

上皮细胞培养有助于定义病理生理学 的CF。组织采购和细胞培养核心在 1984 年，在北卡罗来纳大学 (UNC) 的支持下 囊性纤维化基金会，为囊性纤维化提供标准化细胞培养物 研究人员。核心支持北卡罗来纳大学囊性纤维化项目 研究中心自1988年成立以来，产量和能力不断提高 以满足不断增长的研究需求。组织培养核心将支持 SCOR 项目通过三个主要功能。 1.组织采购。 。从正常组织、疾病控制组织和囊性组织中获取鼻和肺组织 纤维化人类作为气道上皮细胞的来源。 。描述捐献者的诊断、人口统计和临床特征 研究用途所必需的功能。 2.气道上皮细胞分离与培养。 。从人类、灵长类动物的大小气道中分离上皮细胞 和啮齿动物的肺。 。从定义中制备和维持原代上皮细胞培养物 气道区域。 。制备传代或冷冻保存的分化培养物 根据研究者的需要解冻基质上的细胞。 。制备并提供基因敲除的气道上皮细胞系 老鼠。 3.细胞培养技术开发。 。优化上皮培养条件以复制基因表达， 形态分化以及离子和粘液分泌功能 正常和 CF 大、小气道。 。产生基因操纵的、分化良好的初级气道 上皮细胞培养物。 。提供评估气道所需的极高湿度培养环境 在没有蒸发伪影的情况下的表面液体形貌。