Biophysics and Biomechanics of the Semicircular Canals

半规管的生物物理学和生物力学

基本信息

批准号：
8995198
负责人：
RICHARD D RABBITT
金额：
$ 41.02万
依托单位：
UNIVERSITY OF UTAH
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-12-15 至 2018-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8995198
关键词：
Acoustics Afferent Neurons Aminoglycosides Biomechanics Biophysics Brain Brain Stem Cochlea Confocal Microscopy Crista ampullaris Data Deposition Development Disease Ear Elderly Electric Stimulation Endolymph Epithelium Esthesia Exhibits Extracellular Space Extracellular Structure Fluorescence Funding Glycobiology Glycosaminoglycans Goals Hair Hair Cells Health Image Investigation Label Labyrinth Location Maps Measures Mechanics Motion Movement Natural regeneration Neurons Organ Pattern Perilymph Physiological Play Population Process Progress Reports Property Recovery Recovery of Function Relaxation Role Semicircular canal structure Sensory Severities Spatial Distribution Stereocilium Stimulus Surface System Techniques Time Trauma Up-Regulation Work afferent nerve aminoglycoside-induced ototoxicity electric impedance in vivo insight motion sensitivity otoconia patch clamp polysulfated glycosaminoglycan repaired response therapeutic development time use voltage clamp xylosides

项目摘要

DESCRIPTION (provided by applicant): The present work seeks to advance quantitative understanding of semicircular canal biophysics and biomechanics and is organized around three specific aims: 1) Examine sulfated glycosaminoglycans (GAGs) in vivo by tracking the time-course of expression in the cupula and extracellular space around stereocilia following mechanical and aminoglycoside insults. Inner ear GAGs are known to play essential roles in development, mechanics, regeneration, repair, and protection. Our experimental approach using new xyloside conjugates is revealing entirely new information about this important process. 2) Map the spatial distribution of hair bundle displacements across the sensory epithelium in response to physiological stimuli in vivo. The diverse temporal response properties of afferent neurons correlate with projections in the crista ampullaris, but we do not yet know how or if responses depend upon spatial maps of hair bundle displacements. We will measure micromechanical displacement fields while recording afferent responses innervating the same region of the crista. 3) Detail mechano-electrical transduction (MET) current adaptation and its relationship to active amplification by semicircular canal hair cells. Our recent results suggest that the most sensitive semicircular canal afferent neurons rely on hair cell amplification to increase sensitivity to low strength stimuli. We will investigate the role of MET adaptation and electrical mechanisms in this important process by tracking bundle displacements and recording from hair cells in vivo. Results are expected to have long-term impact by enhancing understanding of semicircular canal micromechanics, cupulogenesis and self-repair, amplification by hair cells, and efferent control of motion sensation by the brain.