Microsurgery and Histopathology Core

显微外科和组织病理学核心

• 批准号: 9303260
• 负责人: Zheng J Zhang
• 金额: $ 41.87万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9303260
• 关键词: Address; Advisory Committees; Allografting; Animal Model; Animals; Archives; Area; Biological Preservation; Blood Vessels; Blood specimen; Caring; Cells; Charge; Clinical; Collaborations; Communities; Complex; Cytomegalovirus; Cytomegalovirus Infections; Data Analyses; Development; Drug or chemical Tissue Distribution; Ensure; Epigenetic Process; Evaluation; Goals; Graft Rejection; Heart Transplantation; Histologic; Histological Techniques; Histology; Histopathology; Immunofluorescence Immunologic; Immunofluorescence Microscopy; Immunohistochemistry; Individual; Infection; Inflammation Mediators; Injury; Investigation; Kidney; Kidney Transplantation; Lead; Logistics; Methodological Studies; Methodology; Microsurgery; Modeling; Molecular; Monitor; Mus; Operative Surgical Procedures; Organ Harvestings; Organ Transplantation; Pathologic; Pathologist; Pathology; Pathway interactions; Pharmaceutical Preparations; Positioning Attribute; Procedures; Renal function; Research; Research Design; Research Personnel; Resources; Rodent; Sampling; Services; Site; Species Specificity; Staining method; Stains; Supervision; Surgical Models; Survival Rate; Techniques; Testing; Therapeutic Intervention; Tissue Sample; Tissues; Transplant Recipients; Transplantation; Universities; Work; allograft rejection; animal care; experience; experimental study; flexibility; histological stains; in vivo; insight; interest; light microscopy; meetings; mouse development; mouse model; nephrogenesis; novel strategies; prevent; programs; reactivation from latency; sample collection; sample fixation; skills; success

PROJECT SUMMARY - Microsurgery and Histopathology Core Mouse transplant models are essential for the in vivo studies exploring molecular mechanisms of CMV reactivation following organ transplantation as proposed in this program project. The Microsurgery and Histopathology core (Core A) will serve as a central resource with state-of-the-art microsurgical techniques and histopathological methodologies for the studies proposed in the program projects to help the project leaders achieve their research goals. Core A will providing the necessary skill sets and expertise to allow the use of mouse transplant models with guaranteed technical success and efficiencies. Specifically, Core A will be responsible for performing all mouse transplant surgical procedures, post-transplant care, functional monitoring, and tissue sample collection and processing under Dr. Zhang's direction. Core A will perform histopathological analysis to characterize of allograft injuries (e.g. rejection) on the tissues generated from the Program Project by using state-of-the-art techniques involving histology, immunohistochemistry and immunofluorescence staining with Dr. Kanwar's supervision. Dr. Zhang is the director of the Microsurgery Core at the Northwestern University Comprehensive Transplant Center (NUCTC), a core that has served all interested investigators in the Northwestern University community for more than 15 years with more than 4000 mouse transplant procedures. Dr. Kanwar is an established pathologist with a longstanding track record as an independent investigator in areas of kidney injury has provided important input into our mouse transplant experiments over the past two decades. Both Dr. Zhang and Dr. Kanwar have had a long term and extensive collaboration with the leaders of each project. Core A will be staffed by experienced microsurgeons and lab technologists who have significant experience either in performing rodent vascularized organ transplants or in state-of-the-art histological techniques. It is therefore equipped to perform all the mouse transplant procedures and histopathologic techniques requested by this program project. Furthermore, Core A will be in charge of sample submission to Core B or relevant off-site collaborators for further analyses as needed. It will ensure maximum utilization of the tissue samples, and will facilitate interactions between various projects and between project PIs. Both Dr. Zhang and Dr. Kanwar will participate in study design and data interpretation. They will provide intellectual input specific to how various models can be used to derive mechanistic insights and/or potential therapeutic interventions and will use their expertise to guide the development of novel approaches as desired by the project leaders.

项目摘要 - 显微外科和组织病理学核心 小鼠移植模型对于探索CMV分子机制的体内研究至关重要 该程序项目中提出的器官移植后的重新激活。显微外科手术和 组织病理学核心（核心A）将用最先进的显微外科技术和 计划项目中提出的研究旨在帮助项目领导者的组织病理学方法论 实现他们的研究目标。核心A将提供必要的技能和专业知识，以允许使用 具有保证技术成功和效率的小鼠移植模型。具体来说，核心A将是 负责执行所有小鼠移植手术程序，移植后护理，功能性 在张博士的方向下进行监测以及组织样品的收集和处理。核心A将执行 组织病理学分析以表征同种异体移植损伤（例如排斥）对从 通过使用涉及组织学，免疫组织化学和 通过坎瓦尔博士的监督进行免疫荧光染色。张博士是微外科核心的主任 在西北大学综合移植中心（NUCTC），这是一个为所有人服务的核心 有兴趣的西北大学社区的调查员已有15年以上，超过4000年 鼠标移植程序。 Kanwar博士是一位知名病理学家 肾脏损伤区域的独立研究者为我们的小鼠移植提供了重要的输入 在过去的二十年中进行实验。张博士和坎瓦尔博士都有长期且广泛的 与每个项目的领导者合作。核心A将由经验丰富的微型外科医生和实验室组成 在执行啮齿动物血管化器官移植方面具有丰富经验的技术人员或 最先进的组织学技术。因此，它可以执行所有鼠标移植程序 以及该程序项目要求的组织病理学技术。此外，核心A将负责 示例提交给核心B或相关的非现场合作者，以根据需要进行进一步的分析。它将确保 最大程度地利用组织样品，并将促进各个项目之间的相互作用 PIS项目。 Zhang博士和Kanwar博士都将参加研究设计和数据解释。他们会的 提供特定于如何使用各种模型来得出机械见解和/或的智力输入 潜在的治疗干预措施，并将使用其专业知识来指导新方法的发展 根据项目负责人的需求。