Microsurgery and Histopathology Core

显微外科和组织病理学核心

• 批准号: 8934953
• 负责人: Zheng J Zhang
• 金额: $ 48.27万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8934953
• 关键词: Address; Advisory Committees; Allografting; Animal Model; Animals; Archives; Area; Biological Preservation; Blood specimen; Caring; Cells; Charge; Clinical; Collaborations; Communities; Complex; Core Facility; Cytomegalovirus; Data; Development; Ensure; Epigenetic Process; Evaluation; Goals; Graft Rejection; Heart Transplantation; Histologic; Histological Techniques; Histology; Histopathology; Immunofluorescence Immunologic; Immunofluorescence Microscopy; Immunohistochemistry; Individual; Infection; Inflammation Mediators; Injury; Investigation; Kidney; Kidney Transplantation; Lead; Methodology; Microsurgery; Modeling; Molecular; Monitor; Mus; Operative Surgical Procedures; Organ Harvestings; Organ Transplantation; Pathologist; Pathology; Pathway interactions; Pharmaceutical Preparations; Positioning Attribute; Procedures; Process; Renal function; Research; Research Design; Research Personnel; Resources; Rodent; Sampling; Services; Site; Species Specificity; Staining method; Stains; Supervision; Surgical Models; Survival Rate; Techniques; Testing; Therapeutic Intervention; Tissue Banks; Tissue Sample; Tissues; Transplant Recipients; Transplantation; Universities; Work; allograft rejection; animal care; experience; flexibility; in vivo; insight; interest; light microscopy; meetings; mouse development; mouse model; novel strategies; prevent; programs; research study; sample collection; sample fixation; skills; success

PROJECT SUMMARY - Microsurgery and Histopathology Core Mouse transplant models are essential for the in vivo studies exploring molecular mechanisms of CMV reactivation following organ transplantation as proposed in this program project. The Microsurgery and Histopathology core (Core A) will serve as a central resource with state-of-the-art microsurgical techniques and histopathological methodologies for the studies proposed in the program projects to help the project leaders achieve their research goals. Core A will providing the necessary skill sets and expertise to allow the use of mouse transplant models with guaranteed technical success and efficiencies. Specifically, Core A will be responsible for performing all mouse transplant surgical procedures, post-transplant care, functional monitoring, and tissue sample collection and processing under Dr. Zhang's direction. Core A will perform histopathological analysis to characterize of allograft injuries (e.g. rejection) on the tissues generated from the Program Project by using state-of-the-art techniques involving histology, immunohistochemistry and immunofluorescence staining with Dr. Kanwar's supervision. Dr. Zhang is the director of the Microsurgery Core at the Northwestern University Comprehensive Transplant Center (NUCTC), a core that has served all interested investigators in the Northwestern University community for more than 15 years with more than 4000 mouse transplant procedures. Dr. Kanwar is an established pathologist with a longstanding track record as an independent investigator in areas of kidney injury has provided important input into our mouse transplant experiments over the past two decades. Both Dr. Zhang and Dr. Kanwar have had a long term and extensive collaboration with the leaders of each project. Core A will be staffed by experienced microsurgeons and lab technologists who have significant experience either in performing rodent vascularized organ transplants or in state-of-the-art histological techniques. It is therefore equipped to perform all the mouse transplant procedures and histopathologic techniques requested by this program project. Furthermore, Core A will be in charge of sample submission to Core B or relevant off-site collaborators for further analyses as needed. It will ensure maximum utilization of the tissue samples, and will facilitate interactions between various projects and between project PIs. Both Dr. Zhang and Dr. Kanwar will participate in study design and data interpretation. They will provide intellectual input specific to how various models can be used to derive mechanistic insights and/or potential therapeutic interventions and will use their expertise to guide the development of novel approaches as desired by the project leaders.

项目摘要 - 显微外科和组织病理学核心 小鼠移植模型对于探索 CMV 分子机制的体内研究至关重要 按照本计划项目的建议，器官移植后重新激活。显微外科和 组织病理学核心（核心 A）将作为中心资源，拥有最先进的显微外科技术和 计划项目中提出的研究的组织病理学方法，以帮助项目负责人 实现他们的研究目标。核心 A 将提供必要的技能和专业知识，以允许使用 具有技术成功和效率保证的小鼠移植模型。具体来说，核心A将是 负责执行所有小鼠移植手术程序、移植后护理、功能性 在张博士的指导下进行监测、组织样本采集和处理。核心A将执行 组织病理学分析，以表征从移植物产生的组织上的同种异体移植损伤（例如排斥） 项目项目采用最先进的技术，包括组织学、免疫组织化学和 在 Kanwar 博士的监督下进行免疫荧光染色。张医生担任显微外科核心主任 西北大学综合移植中心 (NUCTC)，该中心为所有患者提供服务 西北大学社区感兴趣的研究人员超过 15 年，超过 4000 名 小鼠移植手术。 Kanwar 博士是一位知名病理学家，拥有长期从事病理学工作的记录。 肾损伤领域的独立研究者为我们的小鼠移植提供了重要的投入 过去二十年的实验。张博士和 Kanwar 博士都拥有长期、广泛的 与每个项目的领导者的合作。核心 A 将由经验丰富的显微外科医生和实验室组成 在进行啮齿动物血管化器官移植或在 最先进的组织学技术。因此它可以执行所有的小鼠移植手术 和该计划项目要求的组织病理学技术。此外，核心A将负责 根据需要将样品提交给核心 B 或相关场外合作者进行进一步分析。它将确保 最大限度地利用组织样本，并将促进各个项目之间以及各个项目之间的互动 项目 PI。张博士和 Kanwar 博士都将参与研究设计和数据解释。他们会 提供特定于如何使用各种模型来得出机械见解的智力输入和/或 潜在的治疗干预措施，并将利用他们的专业知识来指导新方法的开发 正如项目负责人所希望的那样。